Cargando…

Distribution of microbiota in cervical preneoplasia of racially disparate populations

BACKGROUNDS: Microbiome dysbiosis is an important contributing factor in tumor development and thus may be a risk predictor for human malignancies. In the United States, women with Hispanic/Latina (HIS) and African American (AA) background have a higher incidence of cervical cancer and poorer outcom...

Descripción completa

Detalles Bibliográficos
Autores principales: Vikramdeo, Kunwar Somesh, Anand, Shashi, Pierce, Jennifer Young, Singh, Ajay Pratap, Singh, Seema, Dasgupta, Santanu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578267/
https://www.ncbi.nlm.nih.gov/pubmed/36258167
http://dx.doi.org/10.1186/s12885-022-10112-6
_version_ 1784811935944933376
author Vikramdeo, Kunwar Somesh
Anand, Shashi
Pierce, Jennifer Young
Singh, Ajay Pratap
Singh, Seema
Dasgupta, Santanu
author_facet Vikramdeo, Kunwar Somesh
Anand, Shashi
Pierce, Jennifer Young
Singh, Ajay Pratap
Singh, Seema
Dasgupta, Santanu
author_sort Vikramdeo, Kunwar Somesh
collection PubMed
description BACKGROUNDS: Microbiome dysbiosis is an important contributing factor in tumor development and thus may be a risk predictor for human malignancies. In the United States, women with Hispanic/Latina (HIS) and African American (AA) background have a higher incidence of cervical cancer and poorer outcomes than Caucasian American (CA) women. METHODS: Here, we assessed the distribution pattern of microbiota in cervical intraepithelial neoplasia (CIN) lesions obtained from HIS (n = 12), AA (n = 12), and CA (n = 12) women, who were screened for CC risk assessment. We employed a 16S rRNA gene sequencing approach adapted from the NIH-Human Microbiome Project to identify the microbial niche in all CIN lesions (n = 36). RESULTS: We detected an appreciably decreased abundance of beneficial Lactobacillus in the CIN lesions of the AA and HIS women compared to the CA women. Differential abundance of potentially pathogenic Prevotella, Delftia, Gardnerella, and Fastidiosipila was also evident among the various racial groups. An increased abundance of Micrococcus was also evident in AA and HIS women compared to the CA women. The detection level of Rhizobium was higher among the AA ad CA women compared to the HIS women. In addition to the top 10 microbes, a unique niche of 27 microbes was identified exclusively in women with a histopathological diagnosis of CIN. Among these microbes, a group of 8 microbiota; Rubellimicrobium, Podobacter, Brevibacterium, Paracoccus, Atopobium, Brevundimonous, Comamonous, and Novospingobium was detected only in the CIN lesions obtained from AA and CA women. CONCLUSIONS: Microbial dysbiosis in the cervical epithelium represented by an increased ratio of potentially pathogenic to beneficial microbes may be associated with increased CC risk disparities. Developing a race-specific reliable panel of microbial markers could be beneficial for CC risk assessment, disease prevention, and/or therapeutic guidance.
format Online
Article
Text
id pubmed-9578267
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher BioMed Central
record_format MEDLINE/PubMed
spelling pubmed-95782672022-10-19 Distribution of microbiota in cervical preneoplasia of racially disparate populations Vikramdeo, Kunwar Somesh Anand, Shashi Pierce, Jennifer Young Singh, Ajay Pratap Singh, Seema Dasgupta, Santanu BMC Cancer Research BACKGROUNDS: Microbiome dysbiosis is an important contributing factor in tumor development and thus may be a risk predictor for human malignancies. In the United States, women with Hispanic/Latina (HIS) and African American (AA) background have a higher incidence of cervical cancer and poorer outcomes than Caucasian American (CA) women. METHODS: Here, we assessed the distribution pattern of microbiota in cervical intraepithelial neoplasia (CIN) lesions obtained from HIS (n = 12), AA (n = 12), and CA (n = 12) women, who were screened for CC risk assessment. We employed a 16S rRNA gene sequencing approach adapted from the NIH-Human Microbiome Project to identify the microbial niche in all CIN lesions (n = 36). RESULTS: We detected an appreciably decreased abundance of beneficial Lactobacillus in the CIN lesions of the AA and HIS women compared to the CA women. Differential abundance of potentially pathogenic Prevotella, Delftia, Gardnerella, and Fastidiosipila was also evident among the various racial groups. An increased abundance of Micrococcus was also evident in AA and HIS women compared to the CA women. The detection level of Rhizobium was higher among the AA ad CA women compared to the HIS women. In addition to the top 10 microbes, a unique niche of 27 microbes was identified exclusively in women with a histopathological diagnosis of CIN. Among these microbes, a group of 8 microbiota; Rubellimicrobium, Podobacter, Brevibacterium, Paracoccus, Atopobium, Brevundimonous, Comamonous, and Novospingobium was detected only in the CIN lesions obtained from AA and CA women. CONCLUSIONS: Microbial dysbiosis in the cervical epithelium represented by an increased ratio of potentially pathogenic to beneficial microbes may be associated with increased CC risk disparities. Developing a race-specific reliable panel of microbial markers could be beneficial for CC risk assessment, disease prevention, and/or therapeutic guidance. BioMed Central 2022-10-18 /pmc/articles/PMC9578267/ /pubmed/36258167 http://dx.doi.org/10.1186/s12885-022-10112-6 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Vikramdeo, Kunwar Somesh
Anand, Shashi
Pierce, Jennifer Young
Singh, Ajay Pratap
Singh, Seema
Dasgupta, Santanu
Distribution of microbiota in cervical preneoplasia of racially disparate populations
title Distribution of microbiota in cervical preneoplasia of racially disparate populations
title_full Distribution of microbiota in cervical preneoplasia of racially disparate populations
title_fullStr Distribution of microbiota in cervical preneoplasia of racially disparate populations
title_full_unstemmed Distribution of microbiota in cervical preneoplasia of racially disparate populations
title_short Distribution of microbiota in cervical preneoplasia of racially disparate populations
title_sort distribution of microbiota in cervical preneoplasia of racially disparate populations
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578267/
https://www.ncbi.nlm.nih.gov/pubmed/36258167
http://dx.doi.org/10.1186/s12885-022-10112-6
work_keys_str_mv AT vikramdeokunwarsomesh distributionofmicrobiotaincervicalpreneoplasiaofraciallydisparatepopulations
AT anandshashi distributionofmicrobiotaincervicalpreneoplasiaofraciallydisparatepopulations
AT piercejenniferyoung distributionofmicrobiotaincervicalpreneoplasiaofraciallydisparatepopulations
AT singhajaypratap distributionofmicrobiotaincervicalpreneoplasiaofraciallydisparatepopulations
AT singhseema distributionofmicrobiotaincervicalpreneoplasiaofraciallydisparatepopulations
AT dasguptasantanu distributionofmicrobiotaincervicalpreneoplasiaofraciallydisparatepopulations