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Rational domestication of a plant-based recombinant expression system expands its biosynthetic range
Plant molecular farming aims to provide a green, flexible, and rapid alternative to conventional recombinant expression systems, capable of producing complex biologics such as enzymes, vaccines, and antibodies. Historically, the recombinant expression of therapeutic peptides in plants has proven dif...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578353/ https://www.ncbi.nlm.nih.gov/pubmed/35724659 http://dx.doi.org/10.1093/jxb/erac273 |
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author | Jackson, Mark A Chan, Lai Yue Harding, Maxim D Craik, David J Gilding, Edward K |
author_facet | Jackson, Mark A Chan, Lai Yue Harding, Maxim D Craik, David J Gilding, Edward K |
author_sort | Jackson, Mark A |
collection | PubMed |
description | Plant molecular farming aims to provide a green, flexible, and rapid alternative to conventional recombinant expression systems, capable of producing complex biologics such as enzymes, vaccines, and antibodies. Historically, the recombinant expression of therapeutic peptides in plants has proven difficult, largely due to their small size and instability. However, some plant species harbour the capacity for peptide backbone cyclization, a feature inherent in stable therapeutic peptides. One obstacle to realizing the potential of plant-based therapeutic peptide production is the proteolysis of the precursor before it is matured into its final stabilized form. Here we demonstrate the rational domestication of Nicotiana benthamiana within two generations to endow this plant molecular farming host with an expanded repertoire of peptide sequence space. The in planta production of molecules including an insecticidal peptide, a prostate cancer therapeutic lead, and an orally active analgesic is demonstrated. |
format | Online Article Text |
id | pubmed-9578353 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-95783532022-10-19 Rational domestication of a plant-based recombinant expression system expands its biosynthetic range Jackson, Mark A Chan, Lai Yue Harding, Maxim D Craik, David J Gilding, Edward K J Exp Bot Research Papers Plant molecular farming aims to provide a green, flexible, and rapid alternative to conventional recombinant expression systems, capable of producing complex biologics such as enzymes, vaccines, and antibodies. Historically, the recombinant expression of therapeutic peptides in plants has proven difficult, largely due to their small size and instability. However, some plant species harbour the capacity for peptide backbone cyclization, a feature inherent in stable therapeutic peptides. One obstacle to realizing the potential of plant-based therapeutic peptide production is the proteolysis of the precursor before it is matured into its final stabilized form. Here we demonstrate the rational domestication of Nicotiana benthamiana within two generations to endow this plant molecular farming host with an expanded repertoire of peptide sequence space. The in planta production of molecules including an insecticidal peptide, a prostate cancer therapeutic lead, and an orally active analgesic is demonstrated. Oxford University Press 2022-06-20 /pmc/articles/PMC9578353/ /pubmed/35724659 http://dx.doi.org/10.1093/jxb/erac273 Text en © The Author(s) 2022. Published by Oxford University Press on behalf of the Society for Experimental Biology. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Papers Jackson, Mark A Chan, Lai Yue Harding, Maxim D Craik, David J Gilding, Edward K Rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
title | Rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
title_full | Rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
title_fullStr | Rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
title_full_unstemmed | Rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
title_short | Rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
title_sort | rational domestication of a plant-based recombinant expression system expands its biosynthetic range |
topic | Research Papers |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578353/ https://www.ncbi.nlm.nih.gov/pubmed/35724659 http://dx.doi.org/10.1093/jxb/erac273 |
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