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Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs

Knowledge of platelet function in pigs and the effectiveness of antiplatelet therapy is important to ensure proper transferability from animal studies to humans. Our aim was to (1) characterize baseline platelet function of Aachen minipigs using the bedside Multiplate analyzer, (2) compare baseline...

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Autores principales: Franz, Christiane, Bender, Lara, Dorn, Christoph, Sichtermann, Thorsten, Minkenberg, Jan, Franko, Maximilian, Wiesmann, Martin, Stockero, Andrea, Nikoubashman, Omid, May, Rebecca, Ridwan, Hani
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578582/
https://www.ncbi.nlm.nih.gov/pubmed/36256639
http://dx.doi.org/10.1371/journal.pone.0275756
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author Franz, Christiane
Bender, Lara
Dorn, Christoph
Sichtermann, Thorsten
Minkenberg, Jan
Franko, Maximilian
Wiesmann, Martin
Stockero, Andrea
Nikoubashman, Omid
May, Rebecca
Ridwan, Hani
author_facet Franz, Christiane
Bender, Lara
Dorn, Christoph
Sichtermann, Thorsten
Minkenberg, Jan
Franko, Maximilian
Wiesmann, Martin
Stockero, Andrea
Nikoubashman, Omid
May, Rebecca
Ridwan, Hani
author_sort Franz, Christiane
collection PubMed
description Knowledge of platelet function in pigs and the effectiveness of antiplatelet therapy is important to ensure proper transferability from animal studies to humans. Our aim was to (1) characterize baseline platelet function of Aachen minipigs using the bedside Multiplate analyzer, (2) compare baseline platelet function with Göttingen minipigs, and (3) characterize platelet inhibition within the first 5 minutes after intravenous administration of acetylsalicylic acid (ASA). We characterized the baseline platelet function and hematological parameters in 9 Aachen minipigs. Historical data of 8 unmedicated Göttingen minipigs were used for comparison of baseline values. Platelet inhibition in Aachen minipigs was tested 1–5 minutes after intravenous administration of 500 mg ASA. Multiplate examinations included the following tests: ASPI test (to assess the effect of ASA), adenosine-diphosphate-test (ADP test) and thrombin receptor activating peptide test (TRAP test). Median values and interquartile range (IQR) of the Multiplate baseline tests in Aachen minipigs were as follows: ASPI: 39 U (IQR = 21–71), ADP: 70 U (IQR = 48–73), and TRAP: 8 U (IQR = 6–9), whereas the values in Göttingen minipigs were as follows: ASPI: 70.5 U (IQR = 60–78), ADP: 51 U (IQR = 45–66), and TRAP: 6.5 U (IQR = 4–8). ASPI values of Göttingen minipigs were significantly higher than those of Aachen minipigs (p = 0.046). Intravenous administration of ASA in Aachen minipigs resulted in significant platelet inhibition after 1 minute, which remained stable over a period of 5 minutes (p≤0.038). Aachen minipigs appeared to have a high variance in arachidonic acid-mediated platelet aggregation. In Aachen minipigs, intravenous ASA administration resulted in immediate platelet inhibition.
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spelling pubmed-95785822022-10-19 Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs Franz, Christiane Bender, Lara Dorn, Christoph Sichtermann, Thorsten Minkenberg, Jan Franko, Maximilian Wiesmann, Martin Stockero, Andrea Nikoubashman, Omid May, Rebecca Ridwan, Hani PLoS One Research Article Knowledge of platelet function in pigs and the effectiveness of antiplatelet therapy is important to ensure proper transferability from animal studies to humans. Our aim was to (1) characterize baseline platelet function of Aachen minipigs using the bedside Multiplate analyzer, (2) compare baseline platelet function with Göttingen minipigs, and (3) characterize platelet inhibition within the first 5 minutes after intravenous administration of acetylsalicylic acid (ASA). We characterized the baseline platelet function and hematological parameters in 9 Aachen minipigs. Historical data of 8 unmedicated Göttingen minipigs were used for comparison of baseline values. Platelet inhibition in Aachen minipigs was tested 1–5 minutes after intravenous administration of 500 mg ASA. Multiplate examinations included the following tests: ASPI test (to assess the effect of ASA), adenosine-diphosphate-test (ADP test) and thrombin receptor activating peptide test (TRAP test). Median values and interquartile range (IQR) of the Multiplate baseline tests in Aachen minipigs were as follows: ASPI: 39 U (IQR = 21–71), ADP: 70 U (IQR = 48–73), and TRAP: 8 U (IQR = 6–9), whereas the values in Göttingen minipigs were as follows: ASPI: 70.5 U (IQR = 60–78), ADP: 51 U (IQR = 45–66), and TRAP: 6.5 U (IQR = 4–8). ASPI values of Göttingen minipigs were significantly higher than those of Aachen minipigs (p = 0.046). Intravenous administration of ASA in Aachen minipigs resulted in significant platelet inhibition after 1 minute, which remained stable over a period of 5 minutes (p≤0.038). Aachen minipigs appeared to have a high variance in arachidonic acid-mediated platelet aggregation. In Aachen minipigs, intravenous ASA administration resulted in immediate platelet inhibition. Public Library of Science 2022-10-18 /pmc/articles/PMC9578582/ /pubmed/36256639 http://dx.doi.org/10.1371/journal.pone.0275756 Text en © 2022 Franz et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Franz, Christiane
Bender, Lara
Dorn, Christoph
Sichtermann, Thorsten
Minkenberg, Jan
Franko, Maximilian
Wiesmann, Martin
Stockero, Andrea
Nikoubashman, Omid
May, Rebecca
Ridwan, Hani
Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs
title Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs
title_full Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs
title_fullStr Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs
title_full_unstemmed Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs
title_short Platelet function testing using the Multiplate analyzer after administration of aspirin in Aachen minipigs
title_sort platelet function testing using the multiplate analyzer after administration of aspirin in aachen minipigs
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578582/
https://www.ncbi.nlm.nih.gov/pubmed/36256639
http://dx.doi.org/10.1371/journal.pone.0275756
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