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Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction

Natural polymer-based hydrogel films possess considerable potential for use in biomedical applications and are excellent for wound healing. The purpose of this research was to use ionic crosslinking to improve the mechanical characteristics, absorption of fluid in the wound, and drug release behavio...

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Autores principales: Sangkaew, Surat, Wanmasae, Smith, Bunluepeuch, Kingkan, Ongtanasup, Tassanee, Srisang, Siriwan, Manaspon, Chawan, Pooprommin, Philaslak, Eawsakul, Komgrit
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578847/
https://www.ncbi.nlm.nih.gov/pubmed/36267084
http://dx.doi.org/10.1155/2022/4282678
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author Sangkaew, Surat
Wanmasae, Smith
Bunluepeuch, Kingkan
Ongtanasup, Tassanee
Srisang, Siriwan
Manaspon, Chawan
Pooprommin, Philaslak
Eawsakul, Komgrit
author_facet Sangkaew, Surat
Wanmasae, Smith
Bunluepeuch, Kingkan
Ongtanasup, Tassanee
Srisang, Siriwan
Manaspon, Chawan
Pooprommin, Philaslak
Eawsakul, Komgrit
author_sort Sangkaew, Surat
collection PubMed
description Natural polymer-based hydrogel films possess considerable potential for use in biomedical applications and are excellent for wound healing. The purpose of this research was to use ionic crosslinking to improve the mechanical characteristics, absorption of fluid in the wound, and drug release behavior of Cassia alata L. (CA) extract loaded niosomes (CANs) that were incorporated in an alginate-pectin film (A/P). Then, chemically crosslinked A/P hydrogels were obtained by immersing them in different concentrations of calcium chloride (CaCl(2)) (0.5–1% w/v) for 15–120 s. The degree of crosslinking was controlled by both contact time and CaCl(2) concentration. The optimal crosslinking conditions were 1% CaCl(2) for 15 seconds. In this study, the following features of the hydrogel films were investigated: physical properties, morphological characteristics, drug loading, in vitro drug release, antibacterial activity, wound healing activity, cytocompatibility profiles, and hemocompatibility. The crosslinked hydrogel films maintained their physical integrity during use, with the 1% film attaining the best results in the shortest period (15 sec). Then, in vitro drug release from the films was examined. Crosslinking was observed to prolong the release of the CA extract from the hydrogel film. Finally, a cell viability experiment was conducted to evaluate the cytotoxicity profile. The A/P composite film exhibited excellent wound dressing qualities and good mechanical properties in preformulation testing. The in vitro drug release profile indicated that the A/P created a regulated drug release profile, and the cell viability experiment revealed that the film was nontoxic and hemocompatible. A/P composite films can be produced using CAN extract as a possible wound dressing. However, further studies in animals and humans are required to determine both safety and effectiveness.
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spelling pubmed-95788472022-10-19 Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction Sangkaew, Surat Wanmasae, Smith Bunluepeuch, Kingkan Ongtanasup, Tassanee Srisang, Siriwan Manaspon, Chawan Pooprommin, Philaslak Eawsakul, Komgrit Evid Based Complement Alternat Med Research Article Natural polymer-based hydrogel films possess considerable potential for use in biomedical applications and are excellent for wound healing. The purpose of this research was to use ionic crosslinking to improve the mechanical characteristics, absorption of fluid in the wound, and drug release behavior of Cassia alata L. (CA) extract loaded niosomes (CANs) that were incorporated in an alginate-pectin film (A/P). Then, chemically crosslinked A/P hydrogels were obtained by immersing them in different concentrations of calcium chloride (CaCl(2)) (0.5–1% w/v) for 15–120 s. The degree of crosslinking was controlled by both contact time and CaCl(2) concentration. The optimal crosslinking conditions were 1% CaCl(2) for 15 seconds. In this study, the following features of the hydrogel films were investigated: physical properties, morphological characteristics, drug loading, in vitro drug release, antibacterial activity, wound healing activity, cytocompatibility profiles, and hemocompatibility. The crosslinked hydrogel films maintained their physical integrity during use, with the 1% film attaining the best results in the shortest period (15 sec). Then, in vitro drug release from the films was examined. Crosslinking was observed to prolong the release of the CA extract from the hydrogel film. Finally, a cell viability experiment was conducted to evaluate the cytotoxicity profile. The A/P composite film exhibited excellent wound dressing qualities and good mechanical properties in preformulation testing. The in vitro drug release profile indicated that the A/P created a regulated drug release profile, and the cell viability experiment revealed that the film was nontoxic and hemocompatible. A/P composite films can be produced using CAN extract as a possible wound dressing. However, further studies in animals and humans are required to determine both safety and effectiveness. Hindawi 2022-10-11 /pmc/articles/PMC9578847/ /pubmed/36267084 http://dx.doi.org/10.1155/2022/4282678 Text en Copyright © 2022 Surat Sangkaew et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Sangkaew, Surat
Wanmasae, Smith
Bunluepeuch, Kingkan
Ongtanasup, Tassanee
Srisang, Siriwan
Manaspon, Chawan
Pooprommin, Philaslak
Eawsakul, Komgrit
Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction
title Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction
title_full Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction
title_fullStr Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction
title_full_unstemmed Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction
title_short Development of Nanoemulsions for Wound Dressings Containing Cassia alata L. Leaf Extraction
title_sort development of nanoemulsions for wound dressings containing cassia alata l. leaf extraction
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9578847/
https://www.ncbi.nlm.nih.gov/pubmed/36267084
http://dx.doi.org/10.1155/2022/4282678
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