A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay

Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two...

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Detalles Bibliográficos
Autores principales: Sievers, Benjamin Louis, Gelbart, Terri, Tan, Gene S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579189/
https://www.ncbi.nlm.nih.gov/pubmed/36595901
http://dx.doi.org/10.1016/j.xpro.2022.101835
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author Sievers, Benjamin Louis
Gelbart, Terri
Tan, Gene S.
author_facet Sievers, Benjamin Louis
Gelbart, Terri
Tan, Gene S.
author_sort Sievers, Benjamin Louis
collection PubMed
description Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability. For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022).(1)
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spelling pubmed-95791892022-10-19 A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay Sievers, Benjamin Louis Gelbart, Terri Tan, Gene S. STAR Protoc Protocol Evaluating the neutralizing antibody titer following SARS-CoV-2 vaccination is essential in defining correlates of protection. We describe an assay that uses single-cycle vesicular stomatitis virus (VSV) pseudoviruses linking a fluorophore with a spike (S) from a variant of concern (VOC). Using two fluorophores linked to two VOC S, respectively, allows us to determine the neutralization titer against two VOCs in a single run. This is a generalizable approach that saves time, samples, and run-to-run variability. For complete details on the use and execution of this protocol, please refer to Sievers et al. (2022).(1) Elsevier 2022-10-19 /pmc/articles/PMC9579189/ /pubmed/36595901 http://dx.doi.org/10.1016/j.xpro.2022.101835 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Sievers, Benjamin Louis
Gelbart, Terri
Tan, Gene S.
A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
title A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
title_full A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
title_fullStr A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
title_full_unstemmed A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
title_short A high-throughput SARS-CoV-2 pseudovirus multiplex neutralization assay
title_sort high-throughput sars-cov-2 pseudovirus multiplex neutralization assay
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579189/
https://www.ncbi.nlm.nih.gov/pubmed/36595901
http://dx.doi.org/10.1016/j.xpro.2022.101835
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