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A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation

Heparinoid, a sulfate polysaccharide derived from marine organisms was attracted largely attention due to its versatile activities. A naturally occurring heparinoid (M2) that was extracted from the mollusk Meretrix lusoria and used in this investigation shown strong antithrombotic action. UV–Vis, FT...

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Autores principales: Chen, Jing, Du, Zhenxing, Song, Bingbing, Li, Rui, Jia, Xuejing, Chen, Jianping, Liu, Xiaofei, Zhong, Saiyi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579208/
https://www.ncbi.nlm.nih.gov/pubmed/36276241
http://dx.doi.org/10.1016/j.crfs.2022.10.010
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author Chen, Jing
Du, Zhenxing
Song, Bingbing
Li, Rui
Jia, Xuejing
Chen, Jianping
Liu, Xiaofei
Zhong, Saiyi
author_facet Chen, Jing
Du, Zhenxing
Song, Bingbing
Li, Rui
Jia, Xuejing
Chen, Jianping
Liu, Xiaofei
Zhong, Saiyi
author_sort Chen, Jing
collection PubMed
description Heparinoid, a sulfate polysaccharide derived from marine organisms was attracted largely attention due to its versatile activities. A naturally occurring heparinoid (M2) that was extracted from the mollusk Meretrix lusoria and used in this investigation shown strong antithrombotic action. UV–Vis, FT-IR, SAX-HPLC, and NMR were used to explore the structural characteristics of M2, results indicated that M2 similar with heparin, its average molecular weight was 22.58 kDa. Which was primarily made up of→4)-α-IdoA2S-(1→4)-α-GlcNS6S-(1→ (31.19%), →4)-β-GlcA-(1→4)-α-GlcNAc (1→ (23.21%), →4)-β-GlcA-(1→4)-α-GlcNS (1→ (13.87%), →4)-α-IdoA2S-(1→4)-α-GlcNS (1→ (8.95%), →4)-β-GlcA-(1→4)-α-GlcNAc6S (1→ (7.39%) and →4)-β-GlcA-(1→4)-α-GlcNS6S (1→ (7.63%). The antithrombotic activity of M2 was evaluated using measurements of the anticoagulant effect in vitro and the fibrinolytic capability in vitro and in vivo, and M2 has 122.4 U/mg of anticoagulant activity and 1.41 U/mg of fibrinolytic activity, respectively. Additionally, a mouse tail-cutting model was used to assess the bleeding effect in real time, it found that M2 had a reduced hemorrhagic risk than heparin. Consequently, M2 could be exploited to develop functional foods or medications with antithrombotic properties.
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spelling pubmed-95792082022-10-20 A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation Chen, Jing Du, Zhenxing Song, Bingbing Li, Rui Jia, Xuejing Chen, Jianping Liu, Xiaofei Zhong, Saiyi Curr Res Food Sci Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu Heparinoid, a sulfate polysaccharide derived from marine organisms was attracted largely attention due to its versatile activities. A naturally occurring heparinoid (M2) that was extracted from the mollusk Meretrix lusoria and used in this investigation shown strong antithrombotic action. UV–Vis, FT-IR, SAX-HPLC, and NMR were used to explore the structural characteristics of M2, results indicated that M2 similar with heparin, its average molecular weight was 22.58 kDa. Which was primarily made up of→4)-α-IdoA2S-(1→4)-α-GlcNS6S-(1→ (31.19%), →4)-β-GlcA-(1→4)-α-GlcNAc (1→ (23.21%), →4)-β-GlcA-(1→4)-α-GlcNS (1→ (13.87%), →4)-α-IdoA2S-(1→4)-α-GlcNS (1→ (8.95%), →4)-β-GlcA-(1→4)-α-GlcNAc6S (1→ (7.39%) and →4)-β-GlcA-(1→4)-α-GlcNS6S (1→ (7.63%). The antithrombotic activity of M2 was evaluated using measurements of the anticoagulant effect in vitro and the fibrinolytic capability in vitro and in vivo, and M2 has 122.4 U/mg of anticoagulant activity and 1.41 U/mg of fibrinolytic activity, respectively. Additionally, a mouse tail-cutting model was used to assess the bleeding effect in real time, it found that M2 had a reduced hemorrhagic risk than heparin. Consequently, M2 could be exploited to develop functional foods or medications with antithrombotic properties. Elsevier 2022-10-07 /pmc/articles/PMC9579208/ /pubmed/36276241 http://dx.doi.org/10.1016/j.crfs.2022.10.010 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu
Chen, Jing
Du, Zhenxing
Song, Bingbing
Li, Rui
Jia, Xuejing
Chen, Jianping
Liu, Xiaofei
Zhong, Saiyi
A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation
title A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation
title_full A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation
title_fullStr A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation
title_full_unstemmed A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation
title_short A natural heparinoid from mollusc Meretrix lusoria: Purification, structural characterization, and antithrombotic evaluation
title_sort natural heparinoid from mollusc meretrix lusoria: purification, structural characterization, and antithrombotic evaluation
topic Articles from the special issue: 6th International Symposium on Phytochemicals in Medicine and Food, edited by Jianbo Xiao, Jinping Si and Huifan Liu
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579208/
https://www.ncbi.nlm.nih.gov/pubmed/36276241
http://dx.doi.org/10.1016/j.crfs.2022.10.010
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