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Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells

Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Con...

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Autores principales: Exarchos, Vasileios, Neuber, Sebastian, Meyborg, Heike, Giampietro, Costanza, Chala, Nafsika, Moimas, Silvia, Hinkov, Hristian, Kaufmann, Friedrich, Pramotton, Francesca M., Krüger, Katrin, Rodriguez Cetina Biefer, Hector, Cesarovic, Nikola, Poulikakos, Dimos, Falk, Volkmar, Emmert, Maximilian Y., Ferrari, Aldo, Nazari-Shafti, Timo Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579341/
https://www.ncbi.nlm.nih.gov/pubmed/36277782
http://dx.doi.org/10.3389/fcvm.2022.953582
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author Exarchos, Vasileios
Neuber, Sebastian
Meyborg, Heike
Giampietro, Costanza
Chala, Nafsika
Moimas, Silvia
Hinkov, Hristian
Kaufmann, Friedrich
Pramotton, Francesca M.
Krüger, Katrin
Rodriguez Cetina Biefer, Hector
Cesarovic, Nikola
Poulikakos, Dimos
Falk, Volkmar
Emmert, Maximilian Y.
Ferrari, Aldo
Nazari-Shafti, Timo Z.
author_facet Exarchos, Vasileios
Neuber, Sebastian
Meyborg, Heike
Giampietro, Costanza
Chala, Nafsika
Moimas, Silvia
Hinkov, Hristian
Kaufmann, Friedrich
Pramotton, Francesca M.
Krüger, Katrin
Rodriguez Cetina Biefer, Hector
Cesarovic, Nikola
Poulikakos, Dimos
Falk, Volkmar
Emmert, Maximilian Y.
Ferrari, Aldo
Nazari-Shafti, Timo Z.
author_sort Exarchos, Vasileios
collection PubMed
description Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Consequently, extensive cell expansion is necessary, which may eventually lead to replicative senescence. Considering that micro-structured surfaces with anisotropic topography may promote endothelialization, we investigated the impact of gratings on the biomechanical properties and the replicative capacity of senescent ECs. After cultivation on gridded surfaces, the cells showed significant improvements in terms of adherens junction integrity, cell elongation, and orientation of the actin filaments, as well as enhanced yes-associated protein nuclear translocation and cell proliferation. Our data therefore suggest that micro-structured surfaces with anisotropic topographies may improve long-term endothelialization of CVIs.
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spelling pubmed-95793412022-10-20 Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells Exarchos, Vasileios Neuber, Sebastian Meyborg, Heike Giampietro, Costanza Chala, Nafsika Moimas, Silvia Hinkov, Hristian Kaufmann, Friedrich Pramotton, Francesca M. Krüger, Katrin Rodriguez Cetina Biefer, Hector Cesarovic, Nikola Poulikakos, Dimos Falk, Volkmar Emmert, Maximilian Y. Ferrari, Aldo Nazari-Shafti, Timo Z. Front Cardiovasc Med Cardiovascular Medicine Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Consequently, extensive cell expansion is necessary, which may eventually lead to replicative senescence. Considering that micro-structured surfaces with anisotropic topography may promote endothelialization, we investigated the impact of gratings on the biomechanical properties and the replicative capacity of senescent ECs. After cultivation on gridded surfaces, the cells showed significant improvements in terms of adherens junction integrity, cell elongation, and orientation of the actin filaments, as well as enhanced yes-associated protein nuclear translocation and cell proliferation. Our data therefore suggest that micro-structured surfaces with anisotropic topographies may improve long-term endothelialization of CVIs. Frontiers Media S.A. 2022-10-05 /pmc/articles/PMC9579341/ /pubmed/36277782 http://dx.doi.org/10.3389/fcvm.2022.953582 Text en Copyright © 2022 Exarchos, Neuber, Meyborg, Giampietro, Chala, Moimas, Hinkov, Kaufmann, Pramotton, Krüger, Rodriguez Cetina Biefer, Cesarovic, Poulikakos, Falk, Emmert, Ferrari and Nazari-Shafti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Exarchos, Vasileios
Neuber, Sebastian
Meyborg, Heike
Giampietro, Costanza
Chala, Nafsika
Moimas, Silvia
Hinkov, Hristian
Kaufmann, Friedrich
Pramotton, Francesca M.
Krüger, Katrin
Rodriguez Cetina Biefer, Hector
Cesarovic, Nikola
Poulikakos, Dimos
Falk, Volkmar
Emmert, Maximilian Y.
Ferrari, Aldo
Nazari-Shafti, Timo Z.
Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
title Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
title_full Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
title_fullStr Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
title_full_unstemmed Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
title_short Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
title_sort anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579341/
https://www.ncbi.nlm.nih.gov/pubmed/36277782
http://dx.doi.org/10.3389/fcvm.2022.953582
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