Cargando…
Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells
Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Con...
Autores principales: | , , , , , , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579341/ https://www.ncbi.nlm.nih.gov/pubmed/36277782 http://dx.doi.org/10.3389/fcvm.2022.953582 |
_version_ | 1784812159982632960 |
---|---|
author | Exarchos, Vasileios Neuber, Sebastian Meyborg, Heike Giampietro, Costanza Chala, Nafsika Moimas, Silvia Hinkov, Hristian Kaufmann, Friedrich Pramotton, Francesca M. Krüger, Katrin Rodriguez Cetina Biefer, Hector Cesarovic, Nikola Poulikakos, Dimos Falk, Volkmar Emmert, Maximilian Y. Ferrari, Aldo Nazari-Shafti, Timo Z. |
author_facet | Exarchos, Vasileios Neuber, Sebastian Meyborg, Heike Giampietro, Costanza Chala, Nafsika Moimas, Silvia Hinkov, Hristian Kaufmann, Friedrich Pramotton, Francesca M. Krüger, Katrin Rodriguez Cetina Biefer, Hector Cesarovic, Nikola Poulikakos, Dimos Falk, Volkmar Emmert, Maximilian Y. Ferrari, Aldo Nazari-Shafti, Timo Z. |
author_sort | Exarchos, Vasileios |
collection | PubMed |
description | Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Consequently, extensive cell expansion is necessary, which may eventually lead to replicative senescence. Considering that micro-structured surfaces with anisotropic topography may promote endothelialization, we investigated the impact of gratings on the biomechanical properties and the replicative capacity of senescent ECs. After cultivation on gridded surfaces, the cells showed significant improvements in terms of adherens junction integrity, cell elongation, and orientation of the actin filaments, as well as enhanced yes-associated protein nuclear translocation and cell proliferation. Our data therefore suggest that micro-structured surfaces with anisotropic topographies may improve long-term endothelialization of CVIs. |
format | Online Article Text |
id | pubmed-9579341 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95793412022-10-20 Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells Exarchos, Vasileios Neuber, Sebastian Meyborg, Heike Giampietro, Costanza Chala, Nafsika Moimas, Silvia Hinkov, Hristian Kaufmann, Friedrich Pramotton, Francesca M. Krüger, Katrin Rodriguez Cetina Biefer, Hector Cesarovic, Nikola Poulikakos, Dimos Falk, Volkmar Emmert, Maximilian Y. Ferrari, Aldo Nazari-Shafti, Timo Z. Front Cardiovasc Med Cardiovascular Medicine Thrombogenicity remains a major issue in cardiovascular implants (CVIs). Complete surficial coverage of CVIs by a monolayer of endothelial cells (ECs) prior to implantation represents a promising strategy but is hampered by the overall logistical complexity and the high number of cells required. Consequently, extensive cell expansion is necessary, which may eventually lead to replicative senescence. Considering that micro-structured surfaces with anisotropic topography may promote endothelialization, we investigated the impact of gratings on the biomechanical properties and the replicative capacity of senescent ECs. After cultivation on gridded surfaces, the cells showed significant improvements in terms of adherens junction integrity, cell elongation, and orientation of the actin filaments, as well as enhanced yes-associated protein nuclear translocation and cell proliferation. Our data therefore suggest that micro-structured surfaces with anisotropic topographies may improve long-term endothelialization of CVIs. Frontiers Media S.A. 2022-10-05 /pmc/articles/PMC9579341/ /pubmed/36277782 http://dx.doi.org/10.3389/fcvm.2022.953582 Text en Copyright © 2022 Exarchos, Neuber, Meyborg, Giampietro, Chala, Moimas, Hinkov, Kaufmann, Pramotton, Krüger, Rodriguez Cetina Biefer, Cesarovic, Poulikakos, Falk, Emmert, Ferrari and Nazari-Shafti. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cardiovascular Medicine Exarchos, Vasileios Neuber, Sebastian Meyborg, Heike Giampietro, Costanza Chala, Nafsika Moimas, Silvia Hinkov, Hristian Kaufmann, Friedrich Pramotton, Francesca M. Krüger, Katrin Rodriguez Cetina Biefer, Hector Cesarovic, Nikola Poulikakos, Dimos Falk, Volkmar Emmert, Maximilian Y. Ferrari, Aldo Nazari-Shafti, Timo Z. Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
title | Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
title_full | Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
title_fullStr | Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
title_full_unstemmed | Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
title_short | Anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
title_sort | anisotropic topographies restore endothelial monolayer integrity and promote the proliferation of senescent endothelial cells |
topic | Cardiovascular Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579341/ https://www.ncbi.nlm.nih.gov/pubmed/36277782 http://dx.doi.org/10.3389/fcvm.2022.953582 |
work_keys_str_mv | AT exarchosvasileios anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT neubersebastian anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT meyborgheike anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT giampietrocostanza anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT chalanafsika anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT moimassilvia anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT hinkovhristian anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT kaufmannfriedrich anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT pramottonfrancescam anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT krugerkatrin anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT rodriguezcetinabieferhector anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT cesarovicnikola anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT poulikakosdimos anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT falkvolkmar anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT emmertmaximiliany anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT ferrarialdo anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells AT nazarishaftitimoz anisotropictopographiesrestoreendothelialmonolayerintegrityandpromotetheproliferationofsenescentendothelialcells |