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Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo

The global emergence and spread of multi-drug resistant (MDR) strains is becoming increasingly worrisome due to the overuse of broad-spectrum antibiotics. Colistin, the last resort for treating MDR strains infections, has once again returned to the clinician’s choice. However, with the widespread us...

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Autores principales: Wang, Yue, Kong, Jingchun, Zhang, Xiaodong, Liu, Yan, Huang, Zeyu, Yuan, Lu, Zhang, Ying, Cao, Jianming, Chen, Lijiang, Liu, Yong, Zhou, Tieli
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579824/
https://www.ncbi.nlm.nih.gov/pubmed/36274701
http://dx.doi.org/10.3389/fmicb.2022.1020652
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author Wang, Yue
Kong, Jingchun
Zhang, Xiaodong
Liu, Yan
Huang, Zeyu
Yuan, Lu
Zhang, Ying
Cao, Jianming
Chen, Lijiang
Liu, Yong
Zhou, Tieli
author_facet Wang, Yue
Kong, Jingchun
Zhang, Xiaodong
Liu, Yan
Huang, Zeyu
Yuan, Lu
Zhang, Ying
Cao, Jianming
Chen, Lijiang
Liu, Yong
Zhou, Tieli
author_sort Wang, Yue
collection PubMed
description The global emergence and spread of multi-drug resistant (MDR) strains is becoming increasingly worrisome due to the overuse of broad-spectrum antibiotics. Colistin, the last resort for treating MDR strains infections, has once again returned to the clinician’s choice. However, with the widespread use of colistin, colistin-resistant gram-negative bacteria (GNB) have subsequently emerged, including colistin-resistant Pseudomonas aeruginosa (COL-R PA). Therefore, available solutions are urgently needed to respond to this situation. Here, we inspiringly found that the combination of plumbagin and colistin had an efficiently inhibitory effect for colistin-resistant P. aeruginosa in vitro through checkerboard assay and time-kill assay. The combinatorial inhibition of biofilm formation was clearly demonstrated by crystal violet staining and scanning electron microscopy (SEM), and this combination can not only inhibited biofilm formation but also eradicated the mature biofilm. Erythrocytes hemolysis test showed that plumbagin has negligible hemolysis ability. In addition, the increased survival rate of Galleria mellonella (G. mellonella) larva confirmed this combination as same as effective in vivo. As for the mechanism of this combination, propidium iodide (PI) staining showed colistin combined with plumbagin could significantly change the membrane permeability, thus exerting synergistic antibacterial activity. In conclusion, the combination of plumbagin and colistin shows a prominently synergistic antibacterial effect in vitro and in vivo, providing a promising option for the therapy of COL-R PA infection.
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spelling pubmed-95798242022-10-20 Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo Wang, Yue Kong, Jingchun Zhang, Xiaodong Liu, Yan Huang, Zeyu Yuan, Lu Zhang, Ying Cao, Jianming Chen, Lijiang Liu, Yong Zhou, Tieli Front Microbiol Microbiology The global emergence and spread of multi-drug resistant (MDR) strains is becoming increasingly worrisome due to the overuse of broad-spectrum antibiotics. Colistin, the last resort for treating MDR strains infections, has once again returned to the clinician’s choice. However, with the widespread use of colistin, colistin-resistant gram-negative bacteria (GNB) have subsequently emerged, including colistin-resistant Pseudomonas aeruginosa (COL-R PA). Therefore, available solutions are urgently needed to respond to this situation. Here, we inspiringly found that the combination of plumbagin and colistin had an efficiently inhibitory effect for colistin-resistant P. aeruginosa in vitro through checkerboard assay and time-kill assay. The combinatorial inhibition of biofilm formation was clearly demonstrated by crystal violet staining and scanning electron microscopy (SEM), and this combination can not only inhibited biofilm formation but also eradicated the mature biofilm. Erythrocytes hemolysis test showed that plumbagin has negligible hemolysis ability. In addition, the increased survival rate of Galleria mellonella (G. mellonella) larva confirmed this combination as same as effective in vivo. As for the mechanism of this combination, propidium iodide (PI) staining showed colistin combined with plumbagin could significantly change the membrane permeability, thus exerting synergistic antibacterial activity. In conclusion, the combination of plumbagin and colistin shows a prominently synergistic antibacterial effect in vitro and in vivo, providing a promising option for the therapy of COL-R PA infection. Frontiers Media S.A. 2022-09-29 /pmc/articles/PMC9579824/ /pubmed/36274701 http://dx.doi.org/10.3389/fmicb.2022.1020652 Text en Copyright © 2022 Wang, Kong, Zhang, Liu, Huang, Yuan, Zhang, Cao, Chen, Liu and Zhou. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Wang, Yue
Kong, Jingchun
Zhang, Xiaodong
Liu, Yan
Huang, Zeyu
Yuan, Lu
Zhang, Ying
Cao, Jianming
Chen, Lijiang
Liu, Yong
Zhou, Tieli
Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo
title Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo
title_full Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo
title_fullStr Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo
title_full_unstemmed Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo
title_short Plumbagin resurrect colistin susceptible against colistin-resistant Pseudomonas aeruginosa in vitro and in vivo
title_sort plumbagin resurrect colistin susceptible against colistin-resistant pseudomonas aeruginosa in vitro and in vivo
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579824/
https://www.ncbi.nlm.nih.gov/pubmed/36274701
http://dx.doi.org/10.3389/fmicb.2022.1020652
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