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Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs

The European Commission requested scientific and technical assistance in the preparation of a EU‐wide baseline survey protocol for a European Union (EU) coordinated monitoring programme on the prevalence of methicillin‐resistant Staphylococcus Aureus (MRSA) in pigs. The objective of the survey is to...

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Autores principales: Aerts, Marc, Battisti, Antonio, Hendriksen, Rene, Larsen, Jesper, Nilsson, Oskar, Abrahantes, José Cortiñas, Guerra, Beatriz, Papanikolaou, Alexandra, Beloeil, Pierre‐Alexandre
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579990/
https://www.ncbi.nlm.nih.gov/pubmed/36267542
http://dx.doi.org/10.2903/j.efsa.2022.7620
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author Aerts, Marc
Battisti, Antonio
Hendriksen, Rene
Larsen, Jesper
Nilsson, Oskar
Abrahantes, José Cortiñas
Guerra, Beatriz
Papanikolaou, Alexandra
Beloeil, Pierre‐Alexandre
author_facet Aerts, Marc
Battisti, Antonio
Hendriksen, Rene
Larsen, Jesper
Nilsson, Oskar
Abrahantes, José Cortiñas
Guerra, Beatriz
Papanikolaou, Alexandra
Beloeil, Pierre‐Alexandre
collection PubMed
description The European Commission requested scientific and technical assistance in the preparation of a EU‐wide baseline survey protocol for a European Union (EU) coordinated monitoring programme on the prevalence of methicillin‐resistant Staphylococcus Aureus (MRSA) in pigs. The objective of the survey is to estimate the MRSA prevalence in batches of fattening pigs at slaughter at both European and national levels, with a 95% level of confidence and a level of precision of 10% considering an expected prevalence of 50%. The survey protocol defines the target population, the sample size for the survey, sample collection requirements, the analytical methods (for isolation, identification, phenotypic susceptibility testing and further genotypic testing of MRSA isolates), the data reporting requirements and the plan of analysis. The samples are to be analysed according to the laboratory protocols available on the European Union Reference Laboratory (EURL‐AR) website. Generalised linear models will be used to estimate proportion (with 95% confidence intervals) of batches of slaughter pigs tested positive to MRSA. The necessary data to be reported by the EU Member States to support this baseline survey are presented in three data models. The results of the survey should be reported using the EFSA data collection framework.
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spelling pubmed-95799902022-10-19 Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs Aerts, Marc Battisti, Antonio Hendriksen, Rene Larsen, Jesper Nilsson, Oskar Abrahantes, José Cortiñas Guerra, Beatriz Papanikolaou, Alexandra Beloeil, Pierre‐Alexandre EFSA J Scientific Report The European Commission requested scientific and technical assistance in the preparation of a EU‐wide baseline survey protocol for a European Union (EU) coordinated monitoring programme on the prevalence of methicillin‐resistant Staphylococcus Aureus (MRSA) in pigs. The objective of the survey is to estimate the MRSA prevalence in batches of fattening pigs at slaughter at both European and national levels, with a 95% level of confidence and a level of precision of 10% considering an expected prevalence of 50%. The survey protocol defines the target population, the sample size for the survey, sample collection requirements, the analytical methods (for isolation, identification, phenotypic susceptibility testing and further genotypic testing of MRSA isolates), the data reporting requirements and the plan of analysis. The samples are to be analysed according to the laboratory protocols available on the European Union Reference Laboratory (EURL‐AR) website. Generalised linear models will be used to estimate proportion (with 95% confidence intervals) of batches of slaughter pigs tested positive to MRSA. The necessary data to be reported by the EU Member States to support this baseline survey are presented in three data models. The results of the survey should be reported using the EFSA data collection framework. John Wiley and Sons Inc. 2022-10-19 /pmc/articles/PMC9579990/ /pubmed/36267542 http://dx.doi.org/10.2903/j.efsa.2022.7620 Text en © 2022 Wiley‐VCH Verlag GmbH & Co. KgaA on behalf of the European Food Safety Authority. https://creativecommons.org/licenses/by-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nd/4.0/ (https://creativecommons.org/licenses/by-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited and no modifications or adaptations are made.
spellingShingle Scientific Report
Aerts, Marc
Battisti, Antonio
Hendriksen, Rene
Larsen, Jesper
Nilsson, Oskar
Abrahantes, José Cortiñas
Guerra, Beatriz
Papanikolaou, Alexandra
Beloeil, Pierre‐Alexandre
Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs
title Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs
title_full Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs
title_fullStr Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs
title_full_unstemmed Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs
title_short Technical specifications for a baseline survey on the prevalence of methicillin‐resistant Staphylococcus aureus (MRSA) in pigs
title_sort technical specifications for a baseline survey on the prevalence of methicillin‐resistant staphylococcus aureus (mrsa) in pigs
topic Scientific Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9579990/
https://www.ncbi.nlm.nih.gov/pubmed/36267542
http://dx.doi.org/10.2903/j.efsa.2022.7620
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