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Electric field responsive nanotransducers for glioblastoma

BACKGROUND: Electric field therapies such as Tumor Treating Fields (TTFields) have emerged as a bioelectronic treatment for isocitrate dehydrogenase wild-type and IDH mutant grade 4 astrocytoma Glioblastoma (GBM). TTFields rely on alternating current (AC) electric fields (EF) leading to the disrupti...

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Autores principales: Jain, Akhil, Jobson, Isobel, Griffin, Michaela, Rahman, Ruman, Smith, Stuart, Rawson, Frankie J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580136/
https://www.ncbi.nlm.nih.gov/pubmed/36258238
http://dx.doi.org/10.1186/s42234-022-00099-7
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author Jain, Akhil
Jobson, Isobel
Griffin, Michaela
Rahman, Ruman
Smith, Stuart
Rawson, Frankie J.
author_facet Jain, Akhil
Jobson, Isobel
Griffin, Michaela
Rahman, Ruman
Smith, Stuart
Rawson, Frankie J.
author_sort Jain, Akhil
collection PubMed
description BACKGROUND: Electric field therapies such as Tumor Treating Fields (TTFields) have emerged as a bioelectronic treatment for isocitrate dehydrogenase wild-type and IDH mutant grade 4 astrocytoma Glioblastoma (GBM). TTFields rely on alternating current (AC) electric fields (EF) leading to the disruption of dipole alignment and induced dielectrophoresis (DEP) during cytokinesis. Although TTFields have a favourable side effect profile, particularly compared to cytotoxic chemotherapy, survival benefits remain limited (~ 4.9 months) after an extensive treatment regime (20 hours/day for 18 months). The cost of the technology also limits its clinical adoption worldwide. Therefore, the discovery of new technology that can enhance both the therapeutic efficiency and efficacy of these TTFields will be of great benefit to cancer treatment and decrease healthcare costs worldwide. METHODS: In this work, we report the role of electrically conductive gold (GNPs), dielectric silica oxide (SiO(2)), and semiconductor zinc oxide (ZnO) nanoparticles (NPs) as transducers for enhancing EF mediated anticancer effects on patient derived GBM cells. Physicochemical properties of these NPs were analyzed using spectroscopic, electron microscopy, and light-scattering techniques. RESULTS: In vitro TTFields studies indicated an enhanced reduction in the metabolic activity of patient-derived Glioma INvasive marginal (GIN 28) and Glioma contrast enhanced core (GCE 28) GBM As per our journal style, article titles should not include capitalised letters unless these are proper nouns/acronyms. We have therefore used the article title “Electric field responsive nanotransducers for glioblastoma” as opposed to “Electric Field Responsive Nanotransducers for Glioblastoma” as given in the submission system. Please check if this is correct.cells in groups treated with NPs vs. control groups, irrespective of NPs dielectric properties. Our results indicate the inorganic NPs used in this work enhance the intracellular EF effects that could be due to the virtue of bipolar dielectrophoretic and electrophoretic effects. CONCLUSIONS: This work presents preliminary evidence which could help to improve future EF applications for bioelectronic medicine. Furthermore, the merits of spherical morphology, excellent colloidal stability, and low toxicity, make these NPs ideal for future studies for elucidating the detailed mechanism and efficacy upon their delivery in GBM preclinical models. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42234-022-00099-7.
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spelling pubmed-95801362022-10-20 Electric field responsive nanotransducers for glioblastoma Jain, Akhil Jobson, Isobel Griffin, Michaela Rahman, Ruman Smith, Stuart Rawson, Frankie J. Bioelectron Med Research Article BACKGROUND: Electric field therapies such as Tumor Treating Fields (TTFields) have emerged as a bioelectronic treatment for isocitrate dehydrogenase wild-type and IDH mutant grade 4 astrocytoma Glioblastoma (GBM). TTFields rely on alternating current (AC) electric fields (EF) leading to the disruption of dipole alignment and induced dielectrophoresis (DEP) during cytokinesis. Although TTFields have a favourable side effect profile, particularly compared to cytotoxic chemotherapy, survival benefits remain limited (~ 4.9 months) after an extensive treatment regime (20 hours/day for 18 months). The cost of the technology also limits its clinical adoption worldwide. Therefore, the discovery of new technology that can enhance both the therapeutic efficiency and efficacy of these TTFields will be of great benefit to cancer treatment and decrease healthcare costs worldwide. METHODS: In this work, we report the role of electrically conductive gold (GNPs), dielectric silica oxide (SiO(2)), and semiconductor zinc oxide (ZnO) nanoparticles (NPs) as transducers for enhancing EF mediated anticancer effects on patient derived GBM cells. Physicochemical properties of these NPs were analyzed using spectroscopic, electron microscopy, and light-scattering techniques. RESULTS: In vitro TTFields studies indicated an enhanced reduction in the metabolic activity of patient-derived Glioma INvasive marginal (GIN 28) and Glioma contrast enhanced core (GCE 28) GBM As per our journal style, article titles should not include capitalised letters unless these are proper nouns/acronyms. We have therefore used the article title “Electric field responsive nanotransducers for glioblastoma” as opposed to “Electric Field Responsive Nanotransducers for Glioblastoma” as given in the submission system. Please check if this is correct.cells in groups treated with NPs vs. control groups, irrespective of NPs dielectric properties. Our results indicate the inorganic NPs used in this work enhance the intracellular EF effects that could be due to the virtue of bipolar dielectrophoretic and electrophoretic effects. CONCLUSIONS: This work presents preliminary evidence which could help to improve future EF applications for bioelectronic medicine. Furthermore, the merits of spherical morphology, excellent colloidal stability, and low toxicity, make these NPs ideal for future studies for elucidating the detailed mechanism and efficacy upon their delivery in GBM preclinical models. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s42234-022-00099-7. BioMed Central 2022-10-19 /pmc/articles/PMC9580136/ /pubmed/36258238 http://dx.doi.org/10.1186/s42234-022-00099-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Jain, Akhil
Jobson, Isobel
Griffin, Michaela
Rahman, Ruman
Smith, Stuart
Rawson, Frankie J.
Electric field responsive nanotransducers for glioblastoma
title Electric field responsive nanotransducers for glioblastoma
title_full Electric field responsive nanotransducers for glioblastoma
title_fullStr Electric field responsive nanotransducers for glioblastoma
title_full_unstemmed Electric field responsive nanotransducers for glioblastoma
title_short Electric field responsive nanotransducers for glioblastoma
title_sort electric field responsive nanotransducers for glioblastoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580136/
https://www.ncbi.nlm.nih.gov/pubmed/36258238
http://dx.doi.org/10.1186/s42234-022-00099-7
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