A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis

• Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, WB, and IF assays. • A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA. • The mAb, 2G11, shows 97.4% sens...

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Autores principales: Zhu, An-Kai, Li, Sa-Sa, Yu, Shu-Pei, Zhang, Zhao-Yong, Li, Huan, Li, Jing-Feng, Gao, Shan-Shan, Chen, Heng, Zhao, Jincun, Shi, Run, Lu, Mingjian, Li, Chaoyang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Wuhan Institute of Virology, Chinese Academy of Sciences 2022
Materias:
Letter
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580218/
https://www.ncbi.nlm.nih.gov/pubmed/36272711
http://dx.doi.org/10.1016/j.virs.2022.10.003
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author Zhu, An-Kai
Li, Sa-Sa
Yu, Shu-Pei
Zhang, Zhao-Yong
Li, Huan
Li, Jing-Feng
Gao, Shan-Shan
Chen, Heng
Zhao, Jincun
Shi, Run
Lu, Mingjian
Li, Chaoyang
author_facet Zhu, An-Kai
Li, Sa-Sa
Yu, Shu-Pei
Zhang, Zhao-Yong
Li, Huan
Li, Jing-Feng
Gao, Shan-Shan
Chen, Heng
Zhao, Jincun
Shi, Run
Lu, Mingjian
Li, Chaoyang
author_sort Zhu, An-Kai
collection PubMed
description • Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, WB, and IF assays. • A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA. • The mAb, 2G11, shows 97.4% sensitivity and 100% specificity for diagnosing the human blood samples.
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spelling pubmed-95802182022-10-19 A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis Zhu, An-Kai Li, Sa-Sa Yu, Shu-Pei Zhang, Zhao-Yong Li, Huan Li, Jing-Feng Gao, Shan-Shan Chen, Heng Zhao, Jincun Shi, Run Lu, Mingjian Li, Chaoyang Virol Sin Letter • Seven monoclonal antibodies (mAbs) against SARS-CoV-2 nucleocapsid protein are produced, which can be applied in ELISA, WB, and IF assays. • A pair of mAbs, 2G11/bio-1C7, can detect SARS-CoV-2 nucleocapsid protein as low as 15 pg/well in the double sandwich ELISA. • The mAb, 2G11, shows 97.4% sensitivity and 100% specificity for diagnosing the human blood samples. Wuhan Institute of Virology, Chinese Academy of Sciences 2022-10-19 /pmc/articles/PMC9580218/ /pubmed/36272711 http://dx.doi.org/10.1016/j.virs.2022.10.003 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Letter
Zhu, An-Kai
Li, Sa-Sa
Yu, Shu-Pei
Zhang, Zhao-Yong
Li, Huan
Li, Jing-Feng
Gao, Shan-Shan
Chen, Heng
Zhao, Jincun
Shi, Run
Lu, Mingjian
Li, Chaoyang
A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
title A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
title_full A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
title_fullStr A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
title_full_unstemmed A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
title_short A pair of SARS-CoV-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
title_sort pair of sars-cov-2 nucleocapsid protein monoclonal antibodies shows high specificity and sensitivity for diagnosis
topic Letter
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580218/
https://www.ncbi.nlm.nih.gov/pubmed/36272711
http://dx.doi.org/10.1016/j.virs.2022.10.003
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