Cargando…

The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A

Zygotes without a pronuclear (0PN) or with one pronuclear (1PN) were defined as abnormal fertilization in conventional in vitro fertilization (IVF). The removal of 0PN and 1PN zygotes from conventional IVF cycles has always been controversial. This study aimed to investigate the chromosomal aneuploi...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhao, Haijing, Yuan, Ping, Chen, Xiaoli, Lin, Haiyan, Zhao, Jun, Huang, Jia, Qiu, Qi, Ji, Xiaohui, Zhang, Qingxue, Wang, Wenjun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580634/
https://www.ncbi.nlm.nih.gov/pubmed/36303642
http://dx.doi.org/10.3389/frph.2022.966909
_version_ 1784812431850078208
author Zhao, Haijing
Yuan, Ping
Chen, Xiaoli
Lin, Haiyan
Zhao, Jun
Huang, Jia
Qiu, Qi
Ji, Xiaohui
Zhang, Qingxue
Wang, Wenjun
author_facet Zhao, Haijing
Yuan, Ping
Chen, Xiaoli
Lin, Haiyan
Zhao, Jun
Huang, Jia
Qiu, Qi
Ji, Xiaohui
Zhang, Qingxue
Wang, Wenjun
author_sort Zhao, Haijing
collection PubMed
description Zygotes without a pronuclear (0PN) or with one pronuclear (1PN) were defined as abnormal fertilization in conventional in vitro fertilization (IVF). The removal of 0PN and 1PN zygotes from conventional IVF cycles has always been controversial. This study aimed to investigate the chromosomal aneuploidy rates of 0PN- and 1PN-derived blastocysts in conventional IVF cycles and to assess the concordance rate between TE-biopsy PGT-A and miPGT-A. TE biopsies and culture media with blastocoel fluid (CM-BF) samples were whole-genome amplified by multiple annealing and looping-based amplification cycle-based single-cell ChromInst method. Next generation sequencing was performed for comprehensive chromosomal screening on a NextSeq550 sequencer using the NextSeq 500/550 High Output kit v2. The aneuploidy rates of 0PN-derived blastocysts were 19.7% for TE-biopsy PGT-A, and 36.1% for miPGT-A; the concordance rate for ploidy was 77.0%; and the sensitivity and specificity were 83.3% and 75.5%, respectively. The aneuploidy rates of 1PN-derived blastocysts were 37.5% and 37.5% by TE-biopsy PGT-A and miPGT-A, respectively; the concordance rate between TE biopsies and CM-BF samples was 83.3%; and the sensitivity and specificity were 77.8% and 86.7%, respectively. Regarding TE-biopsy PGT-A, there were no significant differences in aneuploidy rates among 0PN-, 1PN- and 2PN-derived blastocysts (PGT-M cycles) (19.7% vs. 37.5% vs. 24.3%, P = 0.226), but the aneuploidy rate of 1PN-derived blastocysts was slightly higher than the other two groups. An increase in aneuploidy rates was observed for 0PN/1PN-derived day 6 blastocysts compared to 0PN/1PN-derived day 5 blastocysts (TE-biopsy PGT-A: 35.7% vs. 19.3%, P = 0.099; miPGT-A: 39.3% vs. 35.1%, P = 0.705). The present study is the first that contributes to understanding the chromosomal aneuploidies in 0PN- and 1PN-derived blastocysts in conventional IVF cycles using TE-biopsy PGT-A and miPGT-A. The clinical application value of 0PN- and 1PN-derived blastocysts in conventional IVF should be assessed using TE-biopsy PGT-A or miPGT-A due to the existence of chromosomal aneuploidies.. In terms of consistency, the miPGT-A using blastocoel fluid enriched culture medium is promising as an alternative to TE-biopsy PGT-A for aneuploidy testing of 0PN- or 1PN-derived blastocysts in conventional IVF.
format Online
Article
Text
id pubmed-9580634
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95806342022-10-26 The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A Zhao, Haijing Yuan, Ping Chen, Xiaoli Lin, Haiyan Zhao, Jun Huang, Jia Qiu, Qi Ji, Xiaohui Zhang, Qingxue Wang, Wenjun Front Reprod Health Reproductive Health Zygotes without a pronuclear (0PN) or with one pronuclear (1PN) were defined as abnormal fertilization in conventional in vitro fertilization (IVF). The removal of 0PN and 1PN zygotes from conventional IVF cycles has always been controversial. This study aimed to investigate the chromosomal aneuploidy rates of 0PN- and 1PN-derived blastocysts in conventional IVF cycles and to assess the concordance rate between TE-biopsy PGT-A and miPGT-A. TE biopsies and culture media with blastocoel fluid (CM-BF) samples were whole-genome amplified by multiple annealing and looping-based amplification cycle-based single-cell ChromInst method. Next generation sequencing was performed for comprehensive chromosomal screening on a NextSeq550 sequencer using the NextSeq 500/550 High Output kit v2. The aneuploidy rates of 0PN-derived blastocysts were 19.7% for TE-biopsy PGT-A, and 36.1% for miPGT-A; the concordance rate for ploidy was 77.0%; and the sensitivity and specificity were 83.3% and 75.5%, respectively. The aneuploidy rates of 1PN-derived blastocysts were 37.5% and 37.5% by TE-biopsy PGT-A and miPGT-A, respectively; the concordance rate between TE biopsies and CM-BF samples was 83.3%; and the sensitivity and specificity were 77.8% and 86.7%, respectively. Regarding TE-biopsy PGT-A, there were no significant differences in aneuploidy rates among 0PN-, 1PN- and 2PN-derived blastocysts (PGT-M cycles) (19.7% vs. 37.5% vs. 24.3%, P = 0.226), but the aneuploidy rate of 1PN-derived blastocysts was slightly higher than the other two groups. An increase in aneuploidy rates was observed for 0PN/1PN-derived day 6 blastocysts compared to 0PN/1PN-derived day 5 blastocysts (TE-biopsy PGT-A: 35.7% vs. 19.3%, P = 0.099; miPGT-A: 39.3% vs. 35.1%, P = 0.705). The present study is the first that contributes to understanding the chromosomal aneuploidies in 0PN- and 1PN-derived blastocysts in conventional IVF cycles using TE-biopsy PGT-A and miPGT-A. The clinical application value of 0PN- and 1PN-derived blastocysts in conventional IVF should be assessed using TE-biopsy PGT-A or miPGT-A due to the existence of chromosomal aneuploidies.. In terms of consistency, the miPGT-A using blastocoel fluid enriched culture medium is promising as an alternative to TE-biopsy PGT-A for aneuploidy testing of 0PN- or 1PN-derived blastocysts in conventional IVF. Frontiers Media S.A. 2022-09-05 /pmc/articles/PMC9580634/ /pubmed/36303642 http://dx.doi.org/10.3389/frph.2022.966909 Text en © 2022 Zhao, Yuan, Chen, Lin, Zhao, Huang, Qiu, Ji, Zhang and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY) (https://creativecommons.org/licenses/by/4.0/) . The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Reproductive Health
Zhao, Haijing
Yuan, Ping
Chen, Xiaoli
Lin, Haiyan
Zhao, Jun
Huang, Jia
Qiu, Qi
Ji, Xiaohui
Zhang, Qingxue
Wang, Wenjun
The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A
title The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A
title_full The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A
title_fullStr The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A
title_full_unstemmed The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A
title_short The aneuploidy testing of blastocysts developing from 0PN and 1PN zygotes in conventional IVF through TE-biopsy PGT-A and minimally invasive PGT-A
title_sort aneuploidy testing of blastocysts developing from 0pn and 1pn zygotes in conventional ivf through te-biopsy pgt-a and minimally invasive pgt-a
topic Reproductive Health
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580634/
https://www.ncbi.nlm.nih.gov/pubmed/36303642
http://dx.doi.org/10.3389/frph.2022.966909
work_keys_str_mv AT zhaohaijing theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT yuanping theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT chenxiaoli theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT linhaiyan theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT zhaojun theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT huangjia theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT qiuqi theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT jixiaohui theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT zhangqingxue theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT wangwenjun theaneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT zhaohaijing aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT yuanping aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT chenxiaoli aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT linhaiyan aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT zhaojun aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT huangjia aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT qiuqi aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT jixiaohui aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT zhangqingxue aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta
AT wangwenjun aneuploidytestingofblastocystsdevelopingfrom0pnand1pnzygotesinconventionalivfthroughtebiopsypgtaandminimallyinvasivepgta