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Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties
Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. Lactobacillus delbrueckii species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strai...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580953/ https://www.ncbi.nlm.nih.gov/pubmed/36304288 http://dx.doi.org/10.3389/fbinf.2022.912795 |
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author | De Jesus, Luís Cláudio Lima Aburjaile, Flávia Figueira Sousa, Thiago De Jesus Felice, Andrei Giacchetto Soares, Siomar De Castro Alcantara, Luiz Carlos Junior Azevedo, Vasco Ariston De Carvalho |
author_facet | De Jesus, Luís Cláudio Lima Aburjaile, Flávia Figueira Sousa, Thiago De Jesus Felice, Andrei Giacchetto Soares, Siomar De Castro Alcantara, Luiz Carlos Junior Azevedo, Vasco Ariston De Carvalho |
author_sort | De Jesus, Luís Cláudio Lima |
collection | PubMed |
description | Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. Lactobacillus delbrueckii species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strains of L. delbrueckii species with documented probiotic properties, focusing on genes related to their host health benefits. For this, a combined methodology including several software and databases (BPGA, SPAAN, BAGEL4, BioCyc, KEEG, and InterSPPI) was used to predict the most important characteristics related to L. delbrueckii strains probiose. Comparative genomics analyses revealed that L. delbrueckii probiotic strains shared essential genes related to acid and bile stress response and antimicrobial activity. Other standard features shared by these strains are surface layer proteins and extracellular proteins-encoding genes, with high adhesion profiles that interacted with human proteins of the inflammatory signaling pathways (TLR2/4-MAPK, TLR2/4-NF-κB, and NOD-like receptors). Among these, the PrtB serine protease appears to be a strong candidate responsible for the anti-inflammatory properties reported for these strains. Furthermore, genes with high proteolytic and metabolic activity able to produce beneficial metabolites, such as acetate, bioactive peptides, and B-complex vitamins were also identified. These findings suggest that these proteins can be essential in biological mechanisms related to probiotics’ beneficial effects of these strains in the host. |
format | Online Article Text |
id | pubmed-9580953 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95809532022-10-26 Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties De Jesus, Luís Cláudio Lima Aburjaile, Flávia Figueira Sousa, Thiago De Jesus Felice, Andrei Giacchetto Soares, Siomar De Castro Alcantara, Luiz Carlos Junior Azevedo, Vasco Ariston De Carvalho Front Bioinform Bioinformatics Probiotics are health-beneficial microorganisms with mainly immunomodulatory and anti-inflammatory properties. Lactobacillus delbrueckii species is a common bacteria used in the dairy industry, and their benefits to hosting health have been reported. This study analyzed the core genome of nine strains of L. delbrueckii species with documented probiotic properties, focusing on genes related to their host health benefits. For this, a combined methodology including several software and databases (BPGA, SPAAN, BAGEL4, BioCyc, KEEG, and InterSPPI) was used to predict the most important characteristics related to L. delbrueckii strains probiose. Comparative genomics analyses revealed that L. delbrueckii probiotic strains shared essential genes related to acid and bile stress response and antimicrobial activity. Other standard features shared by these strains are surface layer proteins and extracellular proteins-encoding genes, with high adhesion profiles that interacted with human proteins of the inflammatory signaling pathways (TLR2/4-MAPK, TLR2/4-NF-κB, and NOD-like receptors). Among these, the PrtB serine protease appears to be a strong candidate responsible for the anti-inflammatory properties reported for these strains. Furthermore, genes with high proteolytic and metabolic activity able to produce beneficial metabolites, such as acetate, bioactive peptides, and B-complex vitamins were also identified. These findings suggest that these proteins can be essential in biological mechanisms related to probiotics’ beneficial effects of these strains in the host. Frontiers Media S.A. 2022-06-06 /pmc/articles/PMC9580953/ /pubmed/36304288 http://dx.doi.org/10.3389/fbinf.2022.912795 Text en Copyright © 2022 De Jesus, Aburjaile, Sousa, Felice, Soares, Alcantara and Azevedo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Bioinformatics De Jesus, Luís Cláudio Lima Aburjaile, Flávia Figueira Sousa, Thiago De Jesus Felice, Andrei Giacchetto Soares, Siomar De Castro Alcantara, Luiz Carlos Junior Azevedo, Vasco Ariston De Carvalho Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties |
title | Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties |
title_full | Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties |
title_fullStr | Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties |
title_full_unstemmed | Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties |
title_short | Genomic Characterization of Lactobacillus delbrueckii Strains with Probiotics Properties |
title_sort | genomic characterization of lactobacillus delbrueckii strains with probiotics properties |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9580953/ https://www.ncbi.nlm.nih.gov/pubmed/36304288 http://dx.doi.org/10.3389/fbinf.2022.912795 |
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