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Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism

Lactic acid bacteria (LAB) can produce a vast spectrum of antifungal metabolites to inhibit fungal growth. The purpose of this study was to elucidate the antifungal effect of isolated Weissella cibaria BYL4.2 on Penicillium chrysogenum, the antifungal activity of W. cibaria BYL4.2 against P. chrysog...

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Autores principales: Yao, Di, Wang, Xiaoyu, Ma, Lixue, Wu, Mengna, Xu, Lei, Yu, Qiaoru, Zhang, Liyuan, Zheng, Xiqun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9581191/
https://www.ncbi.nlm.nih.gov/pubmed/36274712
http://dx.doi.org/10.3389/fmicb.2022.983613
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author Yao, Di
Wang, Xiaoyu
Ma, Lixue
Wu, Mengna
Xu, Lei
Yu, Qiaoru
Zhang, Liyuan
Zheng, Xiqun
author_facet Yao, Di
Wang, Xiaoyu
Ma, Lixue
Wu, Mengna
Xu, Lei
Yu, Qiaoru
Zhang, Liyuan
Zheng, Xiqun
author_sort Yao, Di
collection PubMed
description Lactic acid bacteria (LAB) can produce a vast spectrum of antifungal metabolites to inhibit fungal growth. The purpose of this study was to elucidate the antifungal effect of isolated Weissella cibaria BYL4.2 on Penicillium chrysogenum, the antifungal activity of W. cibaria BYL4.2 against P. chrysogenum was evaluated by the superposition method, results showed that it had obviously antifungal activity against P. chrysogenum. Studying the probiotic properties of BYL4.2 and determining it as beneficial bacteria. Furtherly, different treatments were carried out to characterize the antifungal activity of cell-free supernatant (CFS) produced by W. cibaria BYL4.2, and it was shown that the CFS was pH-dependent, partly heat-sensitive, and was not influenced by proteinaceous treatment. The CFS of W. cibaria BYL4.2 was analyzed by high-performance liquid chromatography (HPLC) and found the highest content of lactic acid. Screening of metabolic markers by a non-targeted metabolomics approach based liquid chromatography-mass spectrometry (LC-MS). The results speculated that organic acid especially detected D-tartaric acid was the main antifungal substance of CFS, which could cause the down-regulation of metabolites in the ABC transporters pathway, thereby inhibiting the growth of P. chrysogenum. Therefore, this study may provide important information for the inhibitory mechanism of W. cibaria BYL4.2 on P. chrysogenum, and provide a basis for further research on the antifungal effect of Weissella.
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spelling pubmed-95811912022-10-20 Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism Yao, Di Wang, Xiaoyu Ma, Lixue Wu, Mengna Xu, Lei Yu, Qiaoru Zhang, Liyuan Zheng, Xiqun Front Microbiol Microbiology Lactic acid bacteria (LAB) can produce a vast spectrum of antifungal metabolites to inhibit fungal growth. The purpose of this study was to elucidate the antifungal effect of isolated Weissella cibaria BYL4.2 on Penicillium chrysogenum, the antifungal activity of W. cibaria BYL4.2 against P. chrysogenum was evaluated by the superposition method, results showed that it had obviously antifungal activity against P. chrysogenum. Studying the probiotic properties of BYL4.2 and determining it as beneficial bacteria. Furtherly, different treatments were carried out to characterize the antifungal activity of cell-free supernatant (CFS) produced by W. cibaria BYL4.2, and it was shown that the CFS was pH-dependent, partly heat-sensitive, and was not influenced by proteinaceous treatment. The CFS of W. cibaria BYL4.2 was analyzed by high-performance liquid chromatography (HPLC) and found the highest content of lactic acid. Screening of metabolic markers by a non-targeted metabolomics approach based liquid chromatography-mass spectrometry (LC-MS). The results speculated that organic acid especially detected D-tartaric acid was the main antifungal substance of CFS, which could cause the down-regulation of metabolites in the ABC transporters pathway, thereby inhibiting the growth of P. chrysogenum. Therefore, this study may provide important information for the inhibitory mechanism of W. cibaria BYL4.2 on P. chrysogenum, and provide a basis for further research on the antifungal effect of Weissella. Frontiers Media S.A. 2022-10-05 /pmc/articles/PMC9581191/ /pubmed/36274712 http://dx.doi.org/10.3389/fmicb.2022.983613 Text en Copyright © 2022 Yao, Wang, Ma, Wu, Xu, Yu, Zhang and Zheng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Yao, Di
Wang, Xiaoyu
Ma, Lixue
Wu, Mengna
Xu, Lei
Yu, Qiaoru
Zhang, Liyuan
Zheng, Xiqun
Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism
title Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism
title_full Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism
title_fullStr Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism
title_full_unstemmed Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism
title_short Impact of Weissella cibaria BYL4.2 and its supernatants on Penicillium chrysogenum metabolism
title_sort impact of weissella cibaria byl4.2 and its supernatants on penicillium chrysogenum metabolism
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9581191/
https://www.ncbi.nlm.nih.gov/pubmed/36274712
http://dx.doi.org/10.3389/fmicb.2022.983613
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