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Lysolipids regulate raft size distribution

The lipid matrix of cellular membranes, directly and indirectly, regulates many vital functions of the cell. The diversity of lipids in membranes leads to the formation of ordered domains called rafts, which play a crucial role in signal transduction, protein sorting and other cellular processes. Ra...

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Autores principales: Krasnobaev, Vladimir D., Galimzyanov, Timur R., Akimov, Sergey A., Batishchev, Oleg V.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9581197/
https://www.ncbi.nlm.nih.gov/pubmed/36275621
http://dx.doi.org/10.3389/fmolb.2022.1021321
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author Krasnobaev, Vladimir D.
Galimzyanov, Timur R.
Akimov, Sergey A.
Batishchev, Oleg V.
author_facet Krasnobaev, Vladimir D.
Galimzyanov, Timur R.
Akimov, Sergey A.
Batishchev, Oleg V.
author_sort Krasnobaev, Vladimir D.
collection PubMed
description The lipid matrix of cellular membranes, directly and indirectly, regulates many vital functions of the cell. The diversity of lipids in membranes leads to the formation of ordered domains called rafts, which play a crucial role in signal transduction, protein sorting and other cellular processes. Rafts are believed to impact the development of different neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, Huntington’s ones, amyotrophic lateral sclerosis, some types of cancer, etc. These diseases correlate with the change in the membrane lipid composition resulting from an oxidative stress, age-related processes, dysfunction of proteins, and many others. In particular, a lot of studies report a significant rise in the level of lysolipids. Physicochemical properties of rafts are determined by membrane composition, in particular, by the content of lysolipids. Lysolipids may thus regulate raft-involving processes. However, the exact mechanism of such regulation is unknown. Although studying rafts in vivo still seems to be rather complicated, liquid-ordered domains are well observed in model systems. In the present study, we used atomic force microscopy (AFM) to examine how lysophospholipids influence the liquid-ordered domains in model ternary membranes. We demonstrated that even a small amount of lysolipids in a membrane significantly impacts domain size depending on the saturation of the lysolipid hydrocarbon tails and the amount of cholesterol. The mixture with the bigger relative fraction of cholesterol was more susceptible to the action of lysolipids. This data helped us to generalize our previous theoretical model of the domain size regulation by lipids with particular molecular shape expanding it to the case of lysolipids and dioleoylglycerol.
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spelling pubmed-95811972022-10-20 Lysolipids regulate raft size distribution Krasnobaev, Vladimir D. Galimzyanov, Timur R. Akimov, Sergey A. Batishchev, Oleg V. Front Mol Biosci Molecular Biosciences The lipid matrix of cellular membranes, directly and indirectly, regulates many vital functions of the cell. The diversity of lipids in membranes leads to the formation of ordered domains called rafts, which play a crucial role in signal transduction, protein sorting and other cellular processes. Rafts are believed to impact the development of different neurodegenerative diseases, such as Alzheimer’s, Parkinson’s, Huntington’s ones, amyotrophic lateral sclerosis, some types of cancer, etc. These diseases correlate with the change in the membrane lipid composition resulting from an oxidative stress, age-related processes, dysfunction of proteins, and many others. In particular, a lot of studies report a significant rise in the level of lysolipids. Physicochemical properties of rafts are determined by membrane composition, in particular, by the content of lysolipids. Lysolipids may thus regulate raft-involving processes. However, the exact mechanism of such regulation is unknown. Although studying rafts in vivo still seems to be rather complicated, liquid-ordered domains are well observed in model systems. In the present study, we used atomic force microscopy (AFM) to examine how lysophospholipids influence the liquid-ordered domains in model ternary membranes. We demonstrated that even a small amount of lysolipids in a membrane significantly impacts domain size depending on the saturation of the lysolipid hydrocarbon tails and the amount of cholesterol. The mixture with the bigger relative fraction of cholesterol was more susceptible to the action of lysolipids. This data helped us to generalize our previous theoretical model of the domain size regulation by lipids with particular molecular shape expanding it to the case of lysolipids and dioleoylglycerol. Frontiers Media S.A. 2022-10-05 /pmc/articles/PMC9581197/ /pubmed/36275621 http://dx.doi.org/10.3389/fmolb.2022.1021321 Text en Copyright © 2022 Krasnobaev, Galimzyanov, Akimov and Batishchev. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Biosciences
Krasnobaev, Vladimir D.
Galimzyanov, Timur R.
Akimov, Sergey A.
Batishchev, Oleg V.
Lysolipids regulate raft size distribution
title Lysolipids regulate raft size distribution
title_full Lysolipids regulate raft size distribution
title_fullStr Lysolipids regulate raft size distribution
title_full_unstemmed Lysolipids regulate raft size distribution
title_short Lysolipids regulate raft size distribution
title_sort lysolipids regulate raft size distribution
topic Molecular Biosciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9581197/
https://www.ncbi.nlm.nih.gov/pubmed/36275621
http://dx.doi.org/10.3389/fmolb.2022.1021321
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