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High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models
Characterization of brain infarct lesions in rodent models of stroke is crucial to assess stroke pathophysiology and therapy outcome. Until recently, the analysis of brain lesions was performed using two techniques: (1) histological methods, such as TTC (Triphenyltetrazolium chloride), a time-consum...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582034/ https://www.ncbi.nlm.nih.gov/pubmed/36261475 http://dx.doi.org/10.1038/s41598-022-21494-9 |
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author | Pinto, Raquel Matula, Jan Gomez-Lazaro, Maria Sousa, Mafalda Lobo, Andrea Zikmund, Tomas Kaiser, Jozef Gomes, João R. |
author_facet | Pinto, Raquel Matula, Jan Gomez-Lazaro, Maria Sousa, Mafalda Lobo, Andrea Zikmund, Tomas Kaiser, Jozef Gomes, João R. |
author_sort | Pinto, Raquel |
collection | PubMed |
description | Characterization of brain infarct lesions in rodent models of stroke is crucial to assess stroke pathophysiology and therapy outcome. Until recently, the analysis of brain lesions was performed using two techniques: (1) histological methods, such as TTC (Triphenyltetrazolium chloride), a time-consuming and inaccurate process; or (2) MRI imaging, a faster, 3D imaging method, that comes at a high cost. In the last decade, high-resolution micro-CT for 3D sample analysis turned into a simple, fast, and cheaper solution. Here, we successfully describe the application of brain contrasting agents (Osmium tetroxide and inorganic iodine) for high-resolution micro-CT imaging for fine location and quantification of ischemic lesion and edema in mouse preclinical stroke models. We used the intraluminal transient MCAO (Middle Cerebral Artery Occlusion) mouse stroke model to identify and quantify ischemic lesion and edema, and segment core and penumbra regions at different time points after ischemia, by manual and automatic methods. In the transient-ischemic-attack (TIA) mouse model, we can quantify striatal myelinated fibers degeneration. Of note, whole brain 3D reconstructions allow brain atlas co-registration, to identify the affected brain areas, and correlate them with functional impairment. This methodology proves to be a breakthrough in the field, by providing a precise and detailed assessment of stroke outcomes in preclinical animal studies. |
format | Online Article Text |
id | pubmed-9582034 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95820342022-10-21 High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models Pinto, Raquel Matula, Jan Gomez-Lazaro, Maria Sousa, Mafalda Lobo, Andrea Zikmund, Tomas Kaiser, Jozef Gomes, João R. Sci Rep Article Characterization of brain infarct lesions in rodent models of stroke is crucial to assess stroke pathophysiology and therapy outcome. Until recently, the analysis of brain lesions was performed using two techniques: (1) histological methods, such as TTC (Triphenyltetrazolium chloride), a time-consuming and inaccurate process; or (2) MRI imaging, a faster, 3D imaging method, that comes at a high cost. In the last decade, high-resolution micro-CT for 3D sample analysis turned into a simple, fast, and cheaper solution. Here, we successfully describe the application of brain contrasting agents (Osmium tetroxide and inorganic iodine) for high-resolution micro-CT imaging for fine location and quantification of ischemic lesion and edema in mouse preclinical stroke models. We used the intraluminal transient MCAO (Middle Cerebral Artery Occlusion) mouse stroke model to identify and quantify ischemic lesion and edema, and segment core and penumbra regions at different time points after ischemia, by manual and automatic methods. In the transient-ischemic-attack (TIA) mouse model, we can quantify striatal myelinated fibers degeneration. Of note, whole brain 3D reconstructions allow brain atlas co-registration, to identify the affected brain areas, and correlate them with functional impairment. This methodology proves to be a breakthrough in the field, by providing a precise and detailed assessment of stroke outcomes in preclinical animal studies. Nature Publishing Group UK 2022-10-19 /pmc/articles/PMC9582034/ /pubmed/36261475 http://dx.doi.org/10.1038/s41598-022-21494-9 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Pinto, Raquel Matula, Jan Gomez-Lazaro, Maria Sousa, Mafalda Lobo, Andrea Zikmund, Tomas Kaiser, Jozef Gomes, João R. High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models |
title | High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models |
title_full | High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models |
title_fullStr | High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models |
title_full_unstemmed | High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models |
title_short | High-resolution micro-CT for 3D infarct characterization and segmentation in mice stroke models |
title_sort | high-resolution micro-ct for 3d infarct characterization and segmentation in mice stroke models |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582034/ https://www.ncbi.nlm.nih.gov/pubmed/36261475 http://dx.doi.org/10.1038/s41598-022-21494-9 |
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