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Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus

INTRODUCTION: Previous studies have demonstrated that exposed to the initial suboptimal intrauterine environment of gestational diabetes mellitus (GDM) may increase risk of cardiovascular disease in adulthood. METHODS: In order to investigate the underlying mechanisms involved in the increased risk...

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Autores principales: Pan, Hai-Tao, Xiong, Yi-Meng, Zhu, Hong-Dan, Shi, Xiao-Liang, Yu, Bin, Ding, Hai-Gang, Xu, Ren-Jie, Ding, Jin-Long, Zhang, Tao, Zhang, Juan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582427/
https://www.ncbi.nlm.nih.gov/pubmed/36277748
http://dx.doi.org/10.3389/fcvm.2022.1021112
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author Pan, Hai-Tao
Xiong, Yi-Meng
Zhu, Hong-Dan
Shi, Xiao-Liang
Yu, Bin
Ding, Hai-Gang
Xu, Ren-Jie
Ding, Jin-Long
Zhang, Tao
Zhang, Juan
author_facet Pan, Hai-Tao
Xiong, Yi-Meng
Zhu, Hong-Dan
Shi, Xiao-Liang
Yu, Bin
Ding, Hai-Gang
Xu, Ren-Jie
Ding, Jin-Long
Zhang, Tao
Zhang, Juan
author_sort Pan, Hai-Tao
collection PubMed
description INTRODUCTION: Previous studies have demonstrated that exposed to the initial suboptimal intrauterine environment of gestational diabetes mellitus (GDM) may increase risk of cardiovascular disease in adulthood. METHODS: In order to investigate the underlying mechanisms involved in the increased risk of cardiovascular diseases (CVDs) in the offspring of GDM, we applied a high-throughput proteomics approach to compare the proteomic expression profile of human umbilical vessels of normal and GDM offspring. RESULTS: A total of significantly different 100 proteins were identified in umbilical vessels from GDM group compared with normal controls, among which 31 proteins were up-regulated, while 69 proteins were down-regulated. Differentially expressed proteins (DEPs) are validated using Western blotting analysis. The analysis of these differently expressed proteins (DEPs) related diseases and functions results, performed by Ingenuity Pathway Analysis (IPA) software. Based on “Diseases and Disorders” analysis, 17 proteins (ACTA2, ADAR, CBFB, DDAH1, FBN1, FGA, FGB, FGG, GLS, GSTM1, HBB, PGM3, PPP1R13L, S100A8, SLC12A4, TPP2, VCAN) were described to be associated with CVD, especially in Anemia, Thrombus and Myocardial infarction. Functional analysis indicated that DEPs involved in many cardiovascular functions, especially in “vasoconstriction of blood vessel” (related DEPs: ACTA2, DDAH1, FBN1, FGA, FGB, and FGG). Upstream regulator analyses of DEPs identifies STAT3 as inhibitor of ACTA2, FGA, FGB, and FGG. CONCLUSION: The results of this study indicate that intrauterine hyperglycemia is associated with an elevated risk of cardiovascular risk in the offspring.
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spelling pubmed-95824272022-10-21 Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus Pan, Hai-Tao Xiong, Yi-Meng Zhu, Hong-Dan Shi, Xiao-Liang Yu, Bin Ding, Hai-Gang Xu, Ren-Jie Ding, Jin-Long Zhang, Tao Zhang, Juan Front Cardiovasc Med Cardiovascular Medicine INTRODUCTION: Previous studies have demonstrated that exposed to the initial suboptimal intrauterine environment of gestational diabetes mellitus (GDM) may increase risk of cardiovascular disease in adulthood. METHODS: In order to investigate the underlying mechanisms involved in the increased risk of cardiovascular diseases (CVDs) in the offspring of GDM, we applied a high-throughput proteomics approach to compare the proteomic expression profile of human umbilical vessels of normal and GDM offspring. RESULTS: A total of significantly different 100 proteins were identified in umbilical vessels from GDM group compared with normal controls, among which 31 proteins were up-regulated, while 69 proteins were down-regulated. Differentially expressed proteins (DEPs) are validated using Western blotting analysis. The analysis of these differently expressed proteins (DEPs) related diseases and functions results, performed by Ingenuity Pathway Analysis (IPA) software. Based on “Diseases and Disorders” analysis, 17 proteins (ACTA2, ADAR, CBFB, DDAH1, FBN1, FGA, FGB, FGG, GLS, GSTM1, HBB, PGM3, PPP1R13L, S100A8, SLC12A4, TPP2, VCAN) were described to be associated with CVD, especially in Anemia, Thrombus and Myocardial infarction. Functional analysis indicated that DEPs involved in many cardiovascular functions, especially in “vasoconstriction of blood vessel” (related DEPs: ACTA2, DDAH1, FBN1, FGA, FGB, and FGG). Upstream regulator analyses of DEPs identifies STAT3 as inhibitor of ACTA2, FGA, FGB, and FGG. CONCLUSION: The results of this study indicate that intrauterine hyperglycemia is associated with an elevated risk of cardiovascular risk in the offspring. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9582427/ /pubmed/36277748 http://dx.doi.org/10.3389/fcvm.2022.1021112 Text en Copyright © 2022 Pan, Xiong, Zhu, Shi, Yu, Ding, Xu, Ding, Zhang and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cardiovascular Medicine
Pan, Hai-Tao
Xiong, Yi-Meng
Zhu, Hong-Dan
Shi, Xiao-Liang
Yu, Bin
Ding, Hai-Gang
Xu, Ren-Jie
Ding, Jin-Long
Zhang, Tao
Zhang, Juan
Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
title Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
title_full Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
title_fullStr Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
title_full_unstemmed Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
title_short Proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
title_sort proteomics and bioinformatics analysis of cardiovascular related proteins in offspring exposed to gestational diabetes mellitus
topic Cardiovascular Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582427/
https://www.ncbi.nlm.nih.gov/pubmed/36277748
http://dx.doi.org/10.3389/fcvm.2022.1021112
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