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On the role of allergen-specific IgG subclasses for blocking human basophil activation

Successful treatment of IgE mediated allergies by allergen-specific immunotherapy (AIT) usually correlates with the induction of allergen-specific IgG4. However, it is not clear whether IgG4 prevents the allergic reaction more efficiently than other IgG subclasses. Here we aimed to compare allergen-...

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Autores principales: Zinkhan, Simon, Thoms, Franziska, Augusto, Gilles, Vogel, Monique, Bachmann, Martin F.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582512/
https://www.ncbi.nlm.nih.gov/pubmed/36275723
http://dx.doi.org/10.3389/fimmu.2022.892631
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author Zinkhan, Simon
Thoms, Franziska
Augusto, Gilles
Vogel, Monique
Bachmann, Martin F.
author_facet Zinkhan, Simon
Thoms, Franziska
Augusto, Gilles
Vogel, Monique
Bachmann, Martin F.
author_sort Zinkhan, Simon
collection PubMed
description Successful treatment of IgE mediated allergies by allergen-specific immunotherapy (AIT) usually correlates with the induction of allergen-specific IgG4. However, it is not clear whether IgG4 prevents the allergic reaction more efficiently than other IgG subclasses. Here we aimed to compare allergen-specific monoclonal IgG1 and IgG4 antibodies in their capacity to inhibit type I allergic reactions by engaging FcγRIIb. We found that IgG1, which is the dominant subclass induced by viruses, binds with a similar affinity to the FcγRIIb as IgG4 and is comparable at blocking human basophil activation from allergic patients; both by neutralizing the allergen as well as engaging the inhibitory receptor FcγRIIb. Hence, the IgG subclass plays a limited role for the protective efficacy of AIT even if IgG4 is considered the best correlate of protection, most likely simply because it is the dominant subclass induced by classical AITs.
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spelling pubmed-95825122022-10-21 On the role of allergen-specific IgG subclasses for blocking human basophil activation Zinkhan, Simon Thoms, Franziska Augusto, Gilles Vogel, Monique Bachmann, Martin F. Front Immunol Immunology Successful treatment of IgE mediated allergies by allergen-specific immunotherapy (AIT) usually correlates with the induction of allergen-specific IgG4. However, it is not clear whether IgG4 prevents the allergic reaction more efficiently than other IgG subclasses. Here we aimed to compare allergen-specific monoclonal IgG1 and IgG4 antibodies in their capacity to inhibit type I allergic reactions by engaging FcγRIIb. We found that IgG1, which is the dominant subclass induced by viruses, binds with a similar affinity to the FcγRIIb as IgG4 and is comparable at blocking human basophil activation from allergic patients; both by neutralizing the allergen as well as engaging the inhibitory receptor FcγRIIb. Hence, the IgG subclass plays a limited role for the protective efficacy of AIT even if IgG4 is considered the best correlate of protection, most likely simply because it is the dominant subclass induced by classical AITs. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9582512/ /pubmed/36275723 http://dx.doi.org/10.3389/fimmu.2022.892631 Text en Copyright © 2022 Zinkhan, Thoms, Augusto, Vogel and Bachmann https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Immunology
Zinkhan, Simon
Thoms, Franziska
Augusto, Gilles
Vogel, Monique
Bachmann, Martin F.
On the role of allergen-specific IgG subclasses for blocking human basophil activation
title On the role of allergen-specific IgG subclasses for blocking human basophil activation
title_full On the role of allergen-specific IgG subclasses for blocking human basophil activation
title_fullStr On the role of allergen-specific IgG subclasses for blocking human basophil activation
title_full_unstemmed On the role of allergen-specific IgG subclasses for blocking human basophil activation
title_short On the role of allergen-specific IgG subclasses for blocking human basophil activation
title_sort on the role of allergen-specific igg subclasses for blocking human basophil activation
topic Immunology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582512/
https://www.ncbi.nlm.nih.gov/pubmed/36275723
http://dx.doi.org/10.3389/fimmu.2022.892631
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