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Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs

BACKGROUND: It is probable that humans can acquire H. pylori and non—H. pylori Helicobacter infections via domestic animals. The prevalence and risk factors of infections of Helicobacter species in canines of Taipei city were therefore analysed in this study. MATERIALS AND METHODS: A total of 95 can...

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Autores principales: Ashaolu, Joseph Opeolu, Tsai, Yu-Jen, Liu, Chia-Chen, Ji, Dar-Der
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582540/
https://www.ncbi.nlm.nih.gov/pubmed/36277106
http://dx.doi.org/10.1016/j.onehlt.2022.100430
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author Ashaolu, Joseph Opeolu
Tsai, Yu-Jen
Liu, Chia-Chen
Ji, Dar-Der
author_facet Ashaolu, Joseph Opeolu
Tsai, Yu-Jen
Liu, Chia-Chen
Ji, Dar-Der
author_sort Ashaolu, Joseph Opeolu
collection PubMed
description BACKGROUND: It is probable that humans can acquire H. pylori and non—H. pylori Helicobacter infections via domestic animals. The prevalence and risk factors of infections of Helicobacter species in canines of Taipei city were therefore analysed in this study. MATERIALS AND METHODS: A total of 95 canine faecal samples were collected from different animal shelters and hospitals in Taipei city. Total DNA was extracted for semi-nested PCR detection of Helicobacter species. The PCR products were sequenced for further comparative database and phylogenetic analyses. RESULTS: The overall prevalence of Helicobacter species in canines of Taipei city was 75.79% (72/95). Two gastric, seven enterohepatic and two unclassified Helicobacter species were identified, all of which have been implicated in the aetiology of human diseases. The predominant species detected included H. canis (27.78%), H. pylori (26.39%), H. canicola (18.06%), and H. bilis (13.89%) in decreasing order, while H. canadensis and H. typhlonius were identified for the first time in canines. The genotypes in H. pylori and H. canicola clusters grouped together, with their respective reference strains, showed a close evolutionary distance in the phylogenetic tree, indicating a common ancestry may have existed in these clusters respectively. The residential region of canines, dog living status (pet or stray) and breed (purebred or mixed-breed) are the risk factors associated with Helicobacter infections in the canines examined. CONCLUSION: The high prevalence of Helicobacter infections in canines highlights a potential public health risk of zoonotic transmission among dogs, humans and other animals, and therefore, the need for proper methods in controlling the transmission routes. In addition, the 16S rRNA gene amplification method was found to be useful for bacterial identification and phylogenetic analysis.
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spelling pubmed-95825402022-10-21 Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs Ashaolu, Joseph Opeolu Tsai, Yu-Jen Liu, Chia-Chen Ji, Dar-Der One Health Research Paper BACKGROUND: It is probable that humans can acquire H. pylori and non—H. pylori Helicobacter infections via domestic animals. The prevalence and risk factors of infections of Helicobacter species in canines of Taipei city were therefore analysed in this study. MATERIALS AND METHODS: A total of 95 canine faecal samples were collected from different animal shelters and hospitals in Taipei city. Total DNA was extracted for semi-nested PCR detection of Helicobacter species. The PCR products were sequenced for further comparative database and phylogenetic analyses. RESULTS: The overall prevalence of Helicobacter species in canines of Taipei city was 75.79% (72/95). Two gastric, seven enterohepatic and two unclassified Helicobacter species were identified, all of which have been implicated in the aetiology of human diseases. The predominant species detected included H. canis (27.78%), H. pylori (26.39%), H. canicola (18.06%), and H. bilis (13.89%) in decreasing order, while H. canadensis and H. typhlonius were identified for the first time in canines. The genotypes in H. pylori and H. canicola clusters grouped together, with their respective reference strains, showed a close evolutionary distance in the phylogenetic tree, indicating a common ancestry may have existed in these clusters respectively. The residential region of canines, dog living status (pet or stray) and breed (purebred or mixed-breed) are the risk factors associated with Helicobacter infections in the canines examined. CONCLUSION: The high prevalence of Helicobacter infections in canines highlights a potential public health risk of zoonotic transmission among dogs, humans and other animals, and therefore, the need for proper methods in controlling the transmission routes. In addition, the 16S rRNA gene amplification method was found to be useful for bacterial identification and phylogenetic analysis. Elsevier 2022-08-29 /pmc/articles/PMC9582540/ /pubmed/36277106 http://dx.doi.org/10.1016/j.onehlt.2022.100430 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Ashaolu, Joseph Opeolu
Tsai, Yu-Jen
Liu, Chia-Chen
Ji, Dar-Der
Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs
title Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs
title_full Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs
title_fullStr Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs
title_full_unstemmed Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs
title_short Prevalence, diversity and public health implications of Helicobacter species in pet and stray dogs
title_sort prevalence, diversity and public health implications of helicobacter species in pet and stray dogs
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582540/
https://www.ncbi.nlm.nih.gov/pubmed/36277106
http://dx.doi.org/10.1016/j.onehlt.2022.100430
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