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Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections

Malaria, dengue and leptospirosis are three tropical infectious diseases that present with severe hematological derangement causing significant morbidity and mortality, especially during the seasonal monsoons. During the course of these infectious diseases, circulating red blood cells are imperiled...

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Autores principales: Jacob, Sanu Susan, Lukose, Jijo, Bankapur, Aseefhali, Mithun, N., Vani Lakshmi, R., Acharya, Mahendra, Rao, Pragna, Kamath, Asha, Baby, Prathap M., Rao, Raghavendra K., Chidangil, Santhosh
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582609/
https://www.ncbi.nlm.nih.gov/pubmed/36275819
http://dx.doi.org/10.3389/fmed.2022.858776
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author Jacob, Sanu Susan
Lukose, Jijo
Bankapur, Aseefhali
Mithun, N.
Vani Lakshmi, R.
Acharya, Mahendra
Rao, Pragna
Kamath, Asha
Baby, Prathap M.
Rao, Raghavendra K.
Chidangil, Santhosh
author_facet Jacob, Sanu Susan
Lukose, Jijo
Bankapur, Aseefhali
Mithun, N.
Vani Lakshmi, R.
Acharya, Mahendra
Rao, Pragna
Kamath, Asha
Baby, Prathap M.
Rao, Raghavendra K.
Chidangil, Santhosh
author_sort Jacob, Sanu Susan
collection PubMed
description Malaria, dengue and leptospirosis are three tropical infectious diseases that present with severe hematological derangement causing significant morbidity and mortality, especially during the seasonal monsoons. During the course of these infectious diseases, circulating red blood cells are imperiled to the direct ill-effects of the infectious pathogen in the body as well as to the pro-inflammatory cytokines generated as a consequence of the infection. RBCs when exposed to such inflammatory and/or pathogenic milieu are susceptible to injuries such as RBC programmed eryptosis or RBC programmed necrosis. This research aimed to explore the Raman spectra of live red cells that were extracted from patients infected with malaria, dengue, and leptospirosis. Red cells were optically trapped and micro-Raman probed using a 785 nm Diode laser. RBCs from samples of all three diseases displayed Raman signatures that were significantly altered from the normal/healthy. Distinct spectral markers that were common across all the four groups were obtained from various standardized multivariate analytical methods. Following comprehensive examination of multiple studies, we propose these spectral wavenumbers as “Raman markers of RBC injury.” Findings in our study display that anemia-triggering infections can inflict variations in the healthy status of red cells, easily identifiable by selectively analyzing specific Raman markers. Additionally, this study also highlights relevant statistical tools that can be utilized to study Raman spectral data from biological samples which could help identify the very significant Raman peaks from the spectral band. This approach of RBC analysis can foster a better understanding of red cell behavior and their alterations exhibited in health and disease.
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spelling pubmed-95826092022-10-21 Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections Jacob, Sanu Susan Lukose, Jijo Bankapur, Aseefhali Mithun, N. Vani Lakshmi, R. Acharya, Mahendra Rao, Pragna Kamath, Asha Baby, Prathap M. Rao, Raghavendra K. Chidangil, Santhosh Front Med (Lausanne) Medicine Malaria, dengue and leptospirosis are three tropical infectious diseases that present with severe hematological derangement causing significant morbidity and mortality, especially during the seasonal monsoons. During the course of these infectious diseases, circulating red blood cells are imperiled to the direct ill-effects of the infectious pathogen in the body as well as to the pro-inflammatory cytokines generated as a consequence of the infection. RBCs when exposed to such inflammatory and/or pathogenic milieu are susceptible to injuries such as RBC programmed eryptosis or RBC programmed necrosis. This research aimed to explore the Raman spectra of live red cells that were extracted from patients infected with malaria, dengue, and leptospirosis. Red cells were optically trapped and micro-Raman probed using a 785 nm Diode laser. RBCs from samples of all three diseases displayed Raman signatures that were significantly altered from the normal/healthy. Distinct spectral markers that were common across all the four groups were obtained from various standardized multivariate analytical methods. Following comprehensive examination of multiple studies, we propose these spectral wavenumbers as “Raman markers of RBC injury.” Findings in our study display that anemia-triggering infections can inflict variations in the healthy status of red cells, easily identifiable by selectively analyzing specific Raman markers. Additionally, this study also highlights relevant statistical tools that can be utilized to study Raman spectral data from biological samples which could help identify the very significant Raman peaks from the spectral band. This approach of RBC analysis can foster a better understanding of red cell behavior and their alterations exhibited in health and disease. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9582609/ /pubmed/36275819 http://dx.doi.org/10.3389/fmed.2022.858776 Text en Copyright © 2022 Jacob, Lukose, Bankapur, Mithun, Vani Lakshmi, Acharya, Rao, Kamath, Baby, Rao and Chidangil. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Medicine
Jacob, Sanu Susan
Lukose, Jijo
Bankapur, Aseefhali
Mithun, N.
Vani Lakshmi, R.
Acharya, Mahendra
Rao, Pragna
Kamath, Asha
Baby, Prathap M.
Rao, Raghavendra K.
Chidangil, Santhosh
Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
title Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
title_full Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
title_fullStr Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
title_full_unstemmed Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
title_short Micro-Raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
title_sort micro-raman spectroscopy study of optically trapped erythrocytes in malaria, dengue and leptospirosis infections
topic Medicine
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582609/
https://www.ncbi.nlm.nih.gov/pubmed/36275819
http://dx.doi.org/10.3389/fmed.2022.858776
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