Cargando…

Confluence and tight junction dependence of volume regulation in epithelial tissue

Epithelial cell volume regulation is a key component to tissue stability and dynamics. In particular, how cells respond to osmotic stresses is of significant physiological interest in kidney epithelial tissue. For individual mammalian cells, it is well established that Na-K-2Cl cotransporter (NKCC)...

Descripción completa

Detalles Bibliográficos
Autores principales: Chmiel, Theresa A., Gardel, Margaret L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582799/
https://www.ncbi.nlm.nih.gov/pubmed/35731553
http://dx.doi.org/10.1091/mbc.E22-03-0073
_version_ 1784812923525267456
author Chmiel, Theresa A.
Gardel, Margaret L.
author_facet Chmiel, Theresa A.
Gardel, Margaret L.
author_sort Chmiel, Theresa A.
collection PubMed
description Epithelial cell volume regulation is a key component to tissue stability and dynamics. In particular, how cells respond to osmotic stresses is of significant physiological interest in kidney epithelial tissue. For individual mammalian cells, it is well established that Na-K-2Cl cotransporter (NKCC) channels mediate cell volume homeostasis in response to hyperosmotic stress. However, whether mature epithelium responds similarly is not well known. Here we show that while small colonies of madin darby canine kidney (MDCK) epithelial cells behave similarly to single cells and exhibit volume homeostasis that is dependent on the NKCC channel function, mature epithelial tissue does not. Instead, the cell volume decreases by 33% when confluent monolayers or acini formed from MDCK cells are subjected to hyperosmotic stress. We show that the tight junction protein zonula occludins-1 (ZO-1), and Rho-associated kinase (ROCK) are essential for osmotic regulation of cell volume in mature epithelium. Because these both are known to be essential for tight junction assembly, this strongly suggests a role for tight junctions in changing volume response in mature epithelium. Thus, tight junctions act either directly or indirectly in osmotic pressure response of epithelial tissue to suppress volume homeostasis common to isolated epithelial cells.
format Online
Article
Text
id pubmed-9582799
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher The American Society for Cell Biology
record_format MEDLINE/PubMed
spelling pubmed-95827992022-11-22 Confluence and tight junction dependence of volume regulation in epithelial tissue Chmiel, Theresa A. Gardel, Margaret L. Mol Biol Cell Articles Epithelial cell volume regulation is a key component to tissue stability and dynamics. In particular, how cells respond to osmotic stresses is of significant physiological interest in kidney epithelial tissue. For individual mammalian cells, it is well established that Na-K-2Cl cotransporter (NKCC) channels mediate cell volume homeostasis in response to hyperosmotic stress. However, whether mature epithelium responds similarly is not well known. Here we show that while small colonies of madin darby canine kidney (MDCK) epithelial cells behave similarly to single cells and exhibit volume homeostasis that is dependent on the NKCC channel function, mature epithelial tissue does not. Instead, the cell volume decreases by 33% when confluent monolayers or acini formed from MDCK cells are subjected to hyperosmotic stress. We show that the tight junction protein zonula occludins-1 (ZO-1), and Rho-associated kinase (ROCK) are essential for osmotic regulation of cell volume in mature epithelium. Because these both are known to be essential for tight junction assembly, this strongly suggests a role for tight junctions in changing volume response in mature epithelium. Thus, tight junctions act either directly or indirectly in osmotic pressure response of epithelial tissue to suppress volume homeostasis common to isolated epithelial cells. The American Society for Cell Biology 2022-09-07 /pmc/articles/PMC9582799/ /pubmed/35731553 http://dx.doi.org/10.1091/mbc.E22-03-0073 Text en © 2022 Chmiel and Gardel. “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology. https://creativecommons.org/licenses/by-nc-sa/3.0/This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial-Share Alike 4.0 International Creative Commons License.
spellingShingle Articles
Chmiel, Theresa A.
Gardel, Margaret L.
Confluence and tight junction dependence of volume regulation in epithelial tissue
title Confluence and tight junction dependence of volume regulation in epithelial tissue
title_full Confluence and tight junction dependence of volume regulation in epithelial tissue
title_fullStr Confluence and tight junction dependence of volume regulation in epithelial tissue
title_full_unstemmed Confluence and tight junction dependence of volume regulation in epithelial tissue
title_short Confluence and tight junction dependence of volume regulation in epithelial tissue
title_sort confluence and tight junction dependence of volume regulation in epithelial tissue
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582799/
https://www.ncbi.nlm.nih.gov/pubmed/35731553
http://dx.doi.org/10.1091/mbc.E22-03-0073
work_keys_str_mv AT chmieltheresaa confluenceandtightjunctiondependenceofvolumeregulationinepithelialtissue
AT gardelmargaretl confluenceandtightjunctiondependenceofvolumeregulationinepithelialtissue