A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters

A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvir...

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Detalles Bibliográficos
Autores principales: Zhang, Baozhu, Yang, Ziyao, Li, Yuxia, Ma, Ling, Li, Fenfang, Lv, Xiuqing, Wen, Guangming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Royal Society of Chemistry 2022
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582908/
https://www.ncbi.nlm.nih.gov/pubmed/36321105
http://dx.doi.org/10.1039/d2ra04636a
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author Zhang, Baozhu
Yang, Ziyao
Li, Yuxia
Ma, Ling
Li, Fenfang
Lv, Xiuqing
Wen, Guangming
author_facet Zhang, Baozhu
Yang, Ziyao
Li, Yuxia
Ma, Ling
Li, Fenfang
Lv, Xiuqing
Wen, Guangming
author_sort Zhang, Baozhu
collection PubMed
description A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6–27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum.
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spelling pubmed-95829082022-10-31 A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters Zhang, Baozhu Yang, Ziyao Li, Yuxia Ma, Ling Li, Fenfang Lv, Xiuqing Wen, Guangming RSC Adv Chemistry A label-free aptasensor has been fabricated in order to detect adenosine triphosphate (ATP) using turn-on fluorescence DNA-Ag NCs. The fluorescence of the DNA-Ag NCs could increase remarkably with the addition of ATP mainly because ATP specifically interacts with its aptamer to change the microenvironment of the darkish DNA-Ag NCs located at one terminus or two termini due to the conformational alteration of the aptamer structure. The proposed sensor can detect ATP in a linear range of 6–27 mM with a good detection limit of 5.0 μM. Additionally, the proposed method succeeded in detecting ATP in fetal bovine serum. The Royal Society of Chemistry 2022-10-20 /pmc/articles/PMC9582908/ /pubmed/36321105 http://dx.doi.org/10.1039/d2ra04636a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by/3.0/
spellingShingle Chemistry
Zhang, Baozhu
Yang, Ziyao
Li, Yuxia
Ma, Ling
Li, Fenfang
Lv, Xiuqing
Wen, Guangming
A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
title A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
title_full A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
title_fullStr A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
title_full_unstemmed A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
title_short A label-free aptasensor for the detection of ATP based on turn-on fluorescence DNA-templated silver nanoclusters
title_sort label-free aptasensor for the detection of atp based on turn-on fluorescence dna-templated silver nanoclusters
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9582908/
https://www.ncbi.nlm.nih.gov/pubmed/36321105
http://dx.doi.org/10.1039/d2ra04636a
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