Development and Validation of a New Robust Detection Method for Low-Content DNA Using ΔΔCq-Based Real-Time PCR with Optimized Standard Plasmids as a Control Sample

[Image: see text] Real-time polymerase chain reaction (PCR) is the gold standard for DNA detection in many fields, including food analysis. However, robust detection using a real-time PCR for low-content DNA samples remains challenging. In this study, we developed a robust real-time PCR method for l...

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Detalles Bibliográficos
Autores principales: Soga, Keisuke, Nakamura, Kosuke, Egi, Tomohiro, Narushima, Jumpei, Yoshiba, Satoko, Kishine, Masahiro, Mano, Junichi, Kitta, Kazumi, Takabatake, Reona, Shibata, Norihito, Kondo, Kazunari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583069/
https://www.ncbi.nlm.nih.gov/pubmed/36205585
http://dx.doi.org/10.1021/acs.analchem.2c03680

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