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Quantifying cell adhesion through forces generated by acoustic streaming
The strength of cell adhesion is important in understanding the cell’s health and in culturing them. Quantitative measurement of cell adhesion strength is a significant challenge in bioengineering research. For this, the present study describes a system that can measure cell adhesion strength using...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583098/ https://www.ncbi.nlm.nih.gov/pubmed/36257212 http://dx.doi.org/10.1016/j.ultsonch.2022.106204 |
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author | Imashiro, Chikahiro Mei, Jiyang Friend, James Takemura, Kenjiro |
author_facet | Imashiro, Chikahiro Mei, Jiyang Friend, James Takemura, Kenjiro |
author_sort | Imashiro, Chikahiro |
collection | PubMed |
description | The strength of cell adhesion is important in understanding the cell’s health and in culturing them. Quantitative measurement of cell adhesion strength is a significant challenge in bioengineering research. For this, the present study describes a system that can measure cell adhesion strength using acoustic streaming induced by Lamb waves. Cells are cultured on an ultrasound transducer using a range of preculture and incubation times with phosphate-buffered saline (PBS) just before the measurement. Acoustic streaming is then induced using several Lamb wave intensities, exposing the cells to shear flows and eventually detaching them. By relying upon a median detachment rate of 50 %, the corresponding detachment force, or force of cell adhesion, was determined to be on the order of several nN, consistent with previous reports. The stronger the induced shear flow, the more cells were detached. Further, we employed a preculture time of 8 to 24 h and a PBS incubation time of 0 to 60 min, producing cell adhesion forces that varied from 1.2 to 13 nN. Hence, the developed system can quantify cell adhesion strength over a wide range, possibly offering a fundamental tool for cell-based bioengineering. |
format | Online Article Text |
id | pubmed-9583098 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-95830982022-10-21 Quantifying cell adhesion through forces generated by acoustic streaming Imashiro, Chikahiro Mei, Jiyang Friend, James Takemura, Kenjiro Ultrason Sonochem Short Communication The strength of cell adhesion is important in understanding the cell’s health and in culturing them. Quantitative measurement of cell adhesion strength is a significant challenge in bioengineering research. For this, the present study describes a system that can measure cell adhesion strength using acoustic streaming induced by Lamb waves. Cells are cultured on an ultrasound transducer using a range of preculture and incubation times with phosphate-buffered saline (PBS) just before the measurement. Acoustic streaming is then induced using several Lamb wave intensities, exposing the cells to shear flows and eventually detaching them. By relying upon a median detachment rate of 50 %, the corresponding detachment force, or force of cell adhesion, was determined to be on the order of several nN, consistent with previous reports. The stronger the induced shear flow, the more cells were detached. Further, we employed a preculture time of 8 to 24 h and a PBS incubation time of 0 to 60 min, producing cell adhesion forces that varied from 1.2 to 13 nN. Hence, the developed system can quantify cell adhesion strength over a wide range, possibly offering a fundamental tool for cell-based bioengineering. Elsevier 2022-10-13 /pmc/articles/PMC9583098/ /pubmed/36257212 http://dx.doi.org/10.1016/j.ultsonch.2022.106204 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Short Communication Imashiro, Chikahiro Mei, Jiyang Friend, James Takemura, Kenjiro Quantifying cell adhesion through forces generated by acoustic streaming |
title | Quantifying cell adhesion through forces generated by acoustic streaming |
title_full | Quantifying cell adhesion through forces generated by acoustic streaming |
title_fullStr | Quantifying cell adhesion through forces generated by acoustic streaming |
title_full_unstemmed | Quantifying cell adhesion through forces generated by acoustic streaming |
title_short | Quantifying cell adhesion through forces generated by acoustic streaming |
title_sort | quantifying cell adhesion through forces generated by acoustic streaming |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583098/ https://www.ncbi.nlm.nih.gov/pubmed/36257212 http://dx.doi.org/10.1016/j.ultsonch.2022.106204 |
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