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Quantifying cell adhesion through forces generated by acoustic streaming

The strength of cell adhesion is important in understanding the cell’s health and in culturing them. Quantitative measurement of cell adhesion strength is a significant challenge in bioengineering research. For this, the present study describes a system that can measure cell adhesion strength using...

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Autores principales: Imashiro, Chikahiro, Mei, Jiyang, Friend, James, Takemura, Kenjiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583098/
https://www.ncbi.nlm.nih.gov/pubmed/36257212
http://dx.doi.org/10.1016/j.ultsonch.2022.106204
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author Imashiro, Chikahiro
Mei, Jiyang
Friend, James
Takemura, Kenjiro
author_facet Imashiro, Chikahiro
Mei, Jiyang
Friend, James
Takemura, Kenjiro
author_sort Imashiro, Chikahiro
collection PubMed
description The strength of cell adhesion is important in understanding the cell’s health and in culturing them. Quantitative measurement of cell adhesion strength is a significant challenge in bioengineering research. For this, the present study describes a system that can measure cell adhesion strength using acoustic streaming induced by Lamb waves. Cells are cultured on an ultrasound transducer using a range of preculture and incubation times with phosphate-buffered saline (PBS) just before the measurement. Acoustic streaming is then induced using several Lamb wave intensities, exposing the cells to shear flows and eventually detaching them. By relying upon a median detachment rate of 50 %, the corresponding detachment force, or force of cell adhesion, was determined to be on the order of several nN, consistent with previous reports. The stronger the induced shear flow, the more cells were detached. Further, we employed a preculture time of 8 to 24 h and a PBS incubation time of 0 to 60 min, producing cell adhesion forces that varied from 1.2 to 13 nN. Hence, the developed system can quantify cell adhesion strength over a wide range, possibly offering a fundamental tool for cell-based bioengineering.
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spelling pubmed-95830982022-10-21 Quantifying cell adhesion through forces generated by acoustic streaming Imashiro, Chikahiro Mei, Jiyang Friend, James Takemura, Kenjiro Ultrason Sonochem Short Communication The strength of cell adhesion is important in understanding the cell’s health and in culturing them. Quantitative measurement of cell adhesion strength is a significant challenge in bioengineering research. For this, the present study describes a system that can measure cell adhesion strength using acoustic streaming induced by Lamb waves. Cells are cultured on an ultrasound transducer using a range of preculture and incubation times with phosphate-buffered saline (PBS) just before the measurement. Acoustic streaming is then induced using several Lamb wave intensities, exposing the cells to shear flows and eventually detaching them. By relying upon a median detachment rate of 50 %, the corresponding detachment force, or force of cell adhesion, was determined to be on the order of several nN, consistent with previous reports. The stronger the induced shear flow, the more cells were detached. Further, we employed a preculture time of 8 to 24 h and a PBS incubation time of 0 to 60 min, producing cell adhesion forces that varied from 1.2 to 13 nN. Hence, the developed system can quantify cell adhesion strength over a wide range, possibly offering a fundamental tool for cell-based bioengineering. Elsevier 2022-10-13 /pmc/articles/PMC9583098/ /pubmed/36257212 http://dx.doi.org/10.1016/j.ultsonch.2022.106204 Text en © 2022 The Authors https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Short Communication
Imashiro, Chikahiro
Mei, Jiyang
Friend, James
Takemura, Kenjiro
Quantifying cell adhesion through forces generated by acoustic streaming
title Quantifying cell adhesion through forces generated by acoustic streaming
title_full Quantifying cell adhesion through forces generated by acoustic streaming
title_fullStr Quantifying cell adhesion through forces generated by acoustic streaming
title_full_unstemmed Quantifying cell adhesion through forces generated by acoustic streaming
title_short Quantifying cell adhesion through forces generated by acoustic streaming
title_sort quantifying cell adhesion through forces generated by acoustic streaming
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583098/
https://www.ncbi.nlm.nih.gov/pubmed/36257212
http://dx.doi.org/10.1016/j.ultsonch.2022.106204
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