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Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort t...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583434/ https://www.ncbi.nlm.nih.gov/pubmed/36278016 http://dx.doi.org/10.3389/fnins.2022.954949 |
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author | Hung, Shih-Te Llobet Rosell, Arnau Jurriens, Daphne Siemons, Marijn Soloviev, Oleg Kapitein, Lukas C. Grußmayer, Kristin Neukomm, Lukas J. Verhaegen, Michel Smith, Carlas |
author_facet | Hung, Shih-Te Llobet Rosell, Arnau Jurriens, Daphne Siemons, Marijn Soloviev, Oleg Kapitein, Lukas C. Grußmayer, Kristin Neukomm, Lukas J. Verhaegen, Michel Smith, Carlas |
author_sort | Hung, Shih-Te |
collection | PubMed |
description | Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue. |
format | Online Article Text |
id | pubmed-9583434 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95834342022-10-21 Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains Hung, Shih-Te Llobet Rosell, Arnau Jurriens, Daphne Siemons, Marijn Soloviev, Oleg Kapitein, Lukas C. Grußmayer, Kristin Neukomm, Lukas J. Verhaegen, Michel Smith, Carlas Front Neurosci Neuroscience Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9583434/ /pubmed/36278016 http://dx.doi.org/10.3389/fnins.2022.954949 Text en Copyright © 2022 Hung, Llobet Rosell, Jurriens, Siemons, Soloviev, Kapitein, Grußmayer, Neukomm, Verhaegen and Smith. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Hung, Shih-Te Llobet Rosell, Arnau Jurriens, Daphne Siemons, Marijn Soloviev, Oleg Kapitein, Lukas C. Grußmayer, Kristin Neukomm, Lukas J. Verhaegen, Michel Smith, Carlas Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains |
title | Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains |
title_full | Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains |
title_fullStr | Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains |
title_full_unstemmed | Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains |
title_short | Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains |
title_sort | adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult drosophila brains |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583434/ https://www.ncbi.nlm.nih.gov/pubmed/36278016 http://dx.doi.org/10.3389/fnins.2022.954949 |
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