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Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains

Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort t...

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Autores principales: Hung, Shih-Te, Llobet Rosell, Arnau, Jurriens, Daphne, Siemons, Marijn, Soloviev, Oleg, Kapitein, Lukas C., Grußmayer, Kristin, Neukomm, Lukas J., Verhaegen, Michel, Smith, Carlas
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583434/
https://www.ncbi.nlm.nih.gov/pubmed/36278016
http://dx.doi.org/10.3389/fnins.2022.954949
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author Hung, Shih-Te
Llobet Rosell, Arnau
Jurriens, Daphne
Siemons, Marijn
Soloviev, Oleg
Kapitein, Lukas C.
Grußmayer, Kristin
Neukomm, Lukas J.
Verhaegen, Michel
Smith, Carlas
author_facet Hung, Shih-Te
Llobet Rosell, Arnau
Jurriens, Daphne
Siemons, Marijn
Soloviev, Oleg
Kapitein, Lukas C.
Grußmayer, Kristin
Neukomm, Lukas J.
Verhaegen, Michel
Smith, Carlas
author_sort Hung, Shih-Te
collection PubMed
description Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue.
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spelling pubmed-95834342022-10-21 Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains Hung, Shih-Te Llobet Rosell, Arnau Jurriens, Daphne Siemons, Marijn Soloviev, Oleg Kapitein, Lukas C. Grußmayer, Kristin Neukomm, Lukas J. Verhaegen, Michel Smith, Carlas Front Neurosci Neuroscience Single-molecule localization microscopy (SMLM) enables the high-resolution visualization of organelle structures and the precise localization of individual proteins. However, the expected resolution is not achieved in tissue as the imaging conditions deteriorate. Sample-induced aberrations distort the point spread function (PSF), and high background fluorescence decreases the localization precision. Here, we synergistically combine sensorless adaptive optics (AO), in-situ 3D-PSF calibration, and a single-objective lens inclined light sheet microscope (SOLEIL), termed (AO-SOLEIL), to mitigate deep tissue-induced deteriorations. We apply AO-SOLEIL on several dSTORM samples including brains of adult Drosophila. We observed a 2x improvement in the estimated axial localization precision with respect to widefield without aberration correction while we used synergistic solution. AO-SOLEIL enhances the overall imaging resolution and further facilitates the visualization of sub-cellular structures in tissue. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9583434/ /pubmed/36278016 http://dx.doi.org/10.3389/fnins.2022.954949 Text en Copyright © 2022 Hung, Llobet Rosell, Jurriens, Siemons, Soloviev, Kapitein, Grußmayer, Neukomm, Verhaegen and Smith. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Hung, Shih-Te
Llobet Rosell, Arnau
Jurriens, Daphne
Siemons, Marijn
Soloviev, Oleg
Kapitein, Lukas C.
Grußmayer, Kristin
Neukomm, Lukas J.
Verhaegen, Michel
Smith, Carlas
Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
title Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
title_full Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
title_fullStr Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
title_full_unstemmed Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
title_short Adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult Drosophila brains
title_sort adaptive optics in single objective inclined light sheet microscopy enables three-dimensional localization microscopy in adult drosophila brains
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583434/
https://www.ncbi.nlm.nih.gov/pubmed/36278016
http://dx.doi.org/10.3389/fnins.2022.954949
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