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Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and mo...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Sciendo
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583443/ https://www.ncbi.nlm.nih.gov/pubmed/36338419 http://dx.doi.org/10.2478/jofnem-2022-0041 |
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author | Kantor, Mihail R. Vieira, Paulo Skantar, Andrea M. Huse, Gregory Handoo, Zafar A. |
author_facet | Kantor, Mihail R. Vieira, Paulo Skantar, Andrea M. Huse, Gregory Handoo, Zafar A. |
author_sort | Kantor, Mihail R. |
collection | PubMed |
description | Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and morphometric characteristics of the recovered fresh material were consistent with the original and other description(s) of this species. The fixed specimens used in this study were preserved in a 3% formaldehyde and 2% glycerin solution for over 20 yr. Molecular analyses of the fresh and fixed specimens were performed using internal transcribed spacer, D2–D2 expansion segments of 28S large subunits, and 18S small subunit ribosomal DNA sequences. To our knowledge, this represents the first report of S. maximus from Virginia and the first report of a successful DNA extraction from fixed nematode specimens. |
format | Online Article Text |
id | pubmed-9583443 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Sciendo |
record_format | MEDLINE/PubMed |
spelling | pubmed-95834432022-11-03 Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA Kantor, Mihail R. Vieira, Paulo Skantar, Andrea M. Huse, Gregory Handoo, Zafar A. J Nematol Research Paper Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and morphometric characteristics of the recovered fresh material were consistent with the original and other description(s) of this species. The fixed specimens used in this study were preserved in a 3% formaldehyde and 2% glycerin solution for over 20 yr. Molecular analyses of the fresh and fixed specimens were performed using internal transcribed spacer, D2–D2 expansion segments of 28S large subunits, and 18S small subunit ribosomal DNA sequences. To our knowledge, this represents the first report of S. maximus from Virginia and the first report of a successful DNA extraction from fixed nematode specimens. Sciendo 2022-10-19 /pmc/articles/PMC9583443/ /pubmed/36338419 http://dx.doi.org/10.2478/jofnem-2022-0041 Text en © 2022 Kantor et al., published by Sciendo https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License. |
spellingShingle | Research Paper Kantor, Mihail R. Vieira, Paulo Skantar, Andrea M. Huse, Gregory Handoo, Zafar A. Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA |
title | Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA |
title_full | Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA |
title_fullStr | Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA |
title_full_unstemmed | Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA |
title_short | Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA |
title_sort | morphological characterization of fresh and 20-yr-old fixed nematode specimens of sauertylenchus maximus (allen, 1955) siddiqi, 2000 deposited in the usda nematode collection from arlington national cemetery, va, usa |
topic | Research Paper |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583443/ https://www.ncbi.nlm.nih.gov/pubmed/36338419 http://dx.doi.org/10.2478/jofnem-2022-0041 |
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