Cargando…

Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA

Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and mo...

Descripción completa

Detalles Bibliográficos
Autores principales: Kantor, Mihail R., Vieira, Paulo, Skantar, Andrea M., Huse, Gregory, Handoo, Zafar A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sciendo 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583443/
https://www.ncbi.nlm.nih.gov/pubmed/36338419
http://dx.doi.org/10.2478/jofnem-2022-0041
_version_ 1784813073417109504
author Kantor, Mihail R.
Vieira, Paulo
Skantar, Andrea M.
Huse, Gregory
Handoo, Zafar A.
author_facet Kantor, Mihail R.
Vieira, Paulo
Skantar, Andrea M.
Huse, Gregory
Handoo, Zafar A.
author_sort Kantor, Mihail R.
collection PubMed
description Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and morphometric characteristics of the recovered fresh material were consistent with the original and other description(s) of this species. The fixed specimens used in this study were preserved in a 3% formaldehyde and 2% glycerin solution for over 20 yr. Molecular analyses of the fresh and fixed specimens were performed using internal transcribed spacer, D2–D2 expansion segments of 28S large subunits, and 18S small subunit ribosomal DNA sequences. To our knowledge, this represents the first report of S. maximus from Virginia and the first report of a successful DNA extraction from fixed nematode specimens.
format Online
Article
Text
id pubmed-9583443
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Sciendo
record_format MEDLINE/PubMed
spelling pubmed-95834432022-11-03 Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA Kantor, Mihail R. Vieira, Paulo Skantar, Andrea M. Huse, Gregory Handoo, Zafar A. J Nematol Research Paper Sauertylenchus maximus was discovered during a survey conducted at the Arlington National Cemetery, Virginia, for the type specimens of Hoplolaimus galeatus. Besides the fresh material, the fixed specimens of S. maximus were also studied by molecular and morphological means. The morphological and morphometric characteristics of the recovered fresh material were consistent with the original and other description(s) of this species. The fixed specimens used in this study were preserved in a 3% formaldehyde and 2% glycerin solution for over 20 yr. Molecular analyses of the fresh and fixed specimens were performed using internal transcribed spacer, D2–D2 expansion segments of 28S large subunits, and 18S small subunit ribosomal DNA sequences. To our knowledge, this represents the first report of S. maximus from Virginia and the first report of a successful DNA extraction from fixed nematode specimens. Sciendo 2022-10-19 /pmc/articles/PMC9583443/ /pubmed/36338419 http://dx.doi.org/10.2478/jofnem-2022-0041 Text en © 2022 Kantor et al., published by Sciendo https://creativecommons.org/licenses/by/4.0/This work is licensed under the Creative Commons Attribution 4.0 International License.
spellingShingle Research Paper
Kantor, Mihail R.
Vieira, Paulo
Skantar, Andrea M.
Huse, Gregory
Handoo, Zafar A.
Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
title Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
title_full Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
title_fullStr Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
title_full_unstemmed Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
title_short Morphological Characterization of Fresh and 20-Yr-Old Fixed Nematode Specimens of Sauertylenchus Maximus (Allen, 1955) Siddiqi, 2000 Deposited in the USDA Nematode Collection from Arlington National Cemetery, VA, USA
title_sort morphological characterization of fresh and 20-yr-old fixed nematode specimens of sauertylenchus maximus (allen, 1955) siddiqi, 2000 deposited in the usda nematode collection from arlington national cemetery, va, usa
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583443/
https://www.ncbi.nlm.nih.gov/pubmed/36338419
http://dx.doi.org/10.2478/jofnem-2022-0041
work_keys_str_mv AT kantormihailr morphologicalcharacterizationoffreshand20yroldfixednematodespecimensofsauertylenchusmaximusallen1955siddiqi2000depositedintheusdanematodecollectionfromarlingtonnationalcemeteryvausa
AT vieirapaulo morphologicalcharacterizationoffreshand20yroldfixednematodespecimensofsauertylenchusmaximusallen1955siddiqi2000depositedintheusdanematodecollectionfromarlingtonnationalcemeteryvausa
AT skantarandream morphologicalcharacterizationoffreshand20yroldfixednematodespecimensofsauertylenchusmaximusallen1955siddiqi2000depositedintheusdanematodecollectionfromarlingtonnationalcemeteryvausa
AT husegregory morphologicalcharacterizationoffreshand20yroldfixednematodespecimensofsauertylenchusmaximusallen1955siddiqi2000depositedintheusdanematodecollectionfromarlingtonnationalcemeteryvausa
AT handoozafara morphologicalcharacterizationoffreshand20yroldfixednematodespecimensofsauertylenchusmaximusallen1955siddiqi2000depositedintheusdanematodecollectionfromarlingtonnationalcemeteryvausa