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Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)

Rice is the staple food for more than half of the world’s population. Iron toxicity limits rice production in several regions of the world. Breeding Fe-tolerant rice varieties is an excellent approach to address the problem of Fe toxicity. Rice responds differently to Fe toxicity at different stages...

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Autores principales: Theerawitaya, Cattarin, Wanchana, Samart, Ruanjaichon, Vinitchan, Tisaram, Rujira, Samphumphuang, Thapanee, Sotesaritkul, Thanyaporn, Cha-um, Suriyan, Toojinda, Theerayut
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583542/
https://www.ncbi.nlm.nih.gov/pubmed/36275605
http://dx.doi.org/10.3389/fpls.2022.994560
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author Theerawitaya, Cattarin
Wanchana, Samart
Ruanjaichon, Vinitchan
Tisaram, Rujira
Samphumphuang, Thapanee
Sotesaritkul, Thanyaporn
Cha-um, Suriyan
Toojinda, Theerayut
author_facet Theerawitaya, Cattarin
Wanchana, Samart
Ruanjaichon, Vinitchan
Tisaram, Rujira
Samphumphuang, Thapanee
Sotesaritkul, Thanyaporn
Cha-um, Suriyan
Toojinda, Theerayut
author_sort Theerawitaya, Cattarin
collection PubMed
description Rice is the staple food for more than half of the world’s population. Iron toxicity limits rice production in several regions of the world. Breeding Fe-tolerant rice varieties is an excellent approach to address the problem of Fe toxicity. Rice responds differently to Fe toxicity at different stages. Most QTLs associated with Fe toxicity have been identified at the seedling stage, and there are very few studies on Fe toxicity across different stages. In this study, we investigated agro-morphological and physiological traits in response to Fe toxicity in a rice diversity panel at seedling, vegetative, and reproductive stages and applied GWAS to identify QTLs/genes associated with these traits. Among agro-morphological and physiological parameters, leaf bronzing score (LBS) is a key parameter for determining Fe toxicity response at all stages, and SDW could be a promising parameter at the seedling stage. A total of 29 QTLs were identified on ten chromosomes. Among them, three colocalized QTLs were identified on chromosome 5, 6, and 11. Several QTLs identified in this study overlapped with previously identified QTLs from bi-parental QTL mapping and association mapping. Two genes previously reported to be associated with iron homeostasis were identified, i.e., LOC_Os01g72370 (OsIRO2, OsbHLH056) and LOC_Os04g38570 (OsABCB14). In addition, based on gene-based haplotype analysis, LOC_Os05g16670 was identified as a candidate gene for the colocalized QTL on chromosome 5 and LOC_Os11g18320 was identified as a candidate gene for the colocalized QTL on chromosome 11. The QTLs and candidate genes identified in this study could be useful for rice breeding programs for Fe toxicity tolerance.
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spelling pubmed-95835422022-10-21 Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.) Theerawitaya, Cattarin Wanchana, Samart Ruanjaichon, Vinitchan Tisaram, Rujira Samphumphuang, Thapanee Sotesaritkul, Thanyaporn Cha-um, Suriyan Toojinda, Theerayut Front Plant Sci Plant Science Rice is the staple food for more than half of the world’s population. Iron toxicity limits rice production in several regions of the world. Breeding Fe-tolerant rice varieties is an excellent approach to address the problem of Fe toxicity. Rice responds differently to Fe toxicity at different stages. Most QTLs associated with Fe toxicity have been identified at the seedling stage, and there are very few studies on Fe toxicity across different stages. In this study, we investigated agro-morphological and physiological traits in response to Fe toxicity in a rice diversity panel at seedling, vegetative, and reproductive stages and applied GWAS to identify QTLs/genes associated with these traits. Among agro-morphological and physiological parameters, leaf bronzing score (LBS) is a key parameter for determining Fe toxicity response at all stages, and SDW could be a promising parameter at the seedling stage. A total of 29 QTLs were identified on ten chromosomes. Among them, three colocalized QTLs were identified on chromosome 5, 6, and 11. Several QTLs identified in this study overlapped with previously identified QTLs from bi-parental QTL mapping and association mapping. Two genes previously reported to be associated with iron homeostasis were identified, i.e., LOC_Os01g72370 (OsIRO2, OsbHLH056) and LOC_Os04g38570 (OsABCB14). In addition, based on gene-based haplotype analysis, LOC_Os05g16670 was identified as a candidate gene for the colocalized QTL on chromosome 5 and LOC_Os11g18320 was identified as a candidate gene for the colocalized QTL on chromosome 11. The QTLs and candidate genes identified in this study could be useful for rice breeding programs for Fe toxicity tolerance. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9583542/ /pubmed/36275605 http://dx.doi.org/10.3389/fpls.2022.994560 Text en Copyright © 2022 Theerawitaya, Wanchana, Ruanjaichon, Tisaram, Samphumphuang, Sotesaritkul, Cha-um and Toojinda. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Theerawitaya, Cattarin
Wanchana, Samart
Ruanjaichon, Vinitchan
Tisaram, Rujira
Samphumphuang, Thapanee
Sotesaritkul, Thanyaporn
Cha-um, Suriyan
Toojinda, Theerayut
Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)
title Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)
title_full Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)
title_fullStr Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)
title_full_unstemmed Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)
title_short Determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (Oryza sativa L.)
title_sort determination of traits responding to iron toxicity stress at different stages and genome-wide association analysis for iron toxicity tolerance in rice (oryza sativa l.)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583542/
https://www.ncbi.nlm.nih.gov/pubmed/36275605
http://dx.doi.org/10.3389/fpls.2022.994560
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