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Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain

Visualizing the relationships and interactions among different biological components in the whole brain is crucial to our understanding of brain structures and functions. However, an automatic multicolor whole-brain imaging technique is still lacking. Here, we developed a multicolor wide-field large...

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Autores principales: Ding, Zhangheng, Zhao, Jiangjiang, Luo, Tianpeng, Lu, Bolin, Zhang, Xiaoyu, Chen, Siqi, Li, Anan, Jia, Xueyan, Zhang, Jianmin, Chen, Wu, Chen, Jianwei, Sun, Qingtao, Li, Xiangning, Gong, Hui, Yuan, Jing
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583816/
https://www.ncbi.nlm.nih.gov/pubmed/36278018
http://dx.doi.org/10.3389/fnins.2022.1033880
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author Ding, Zhangheng
Zhao, Jiangjiang
Luo, Tianpeng
Lu, Bolin
Zhang, Xiaoyu
Chen, Siqi
Li, Anan
Jia, Xueyan
Zhang, Jianmin
Chen, Wu
Chen, Jianwei
Sun, Qingtao
Li, Xiangning
Gong, Hui
Yuan, Jing
author_facet Ding, Zhangheng
Zhao, Jiangjiang
Luo, Tianpeng
Lu, Bolin
Zhang, Xiaoyu
Chen, Siqi
Li, Anan
Jia, Xueyan
Zhang, Jianmin
Chen, Wu
Chen, Jianwei
Sun, Qingtao
Li, Xiangning
Gong, Hui
Yuan, Jing
author_sort Ding, Zhangheng
collection PubMed
description Visualizing the relationships and interactions among different biological components in the whole brain is crucial to our understanding of brain structures and functions. However, an automatic multicolor whole-brain imaging technique is still lacking. Here, we developed a multicolor wide-field large-volume tomography (multicolor WVT) to simultaneously acquire fluorescent signals in blue, green, and red channels in the whole brain. To facilitate the segmentation of brain regions and anatomical annotation, we used 4′, 6-diamidino-2-phenylindole (DAPI) to provide cytoarchitecture through real-time counterstaining. We optimized the imaging planes and modes of three channels to overcome the axial chromatic aberration of the illumination path and avoid the crosstalk from DAPI to the green channel without the modification of system configuration. We also developed an automatic contour recognition algorithm based on DAPI-staining cytoarchitecture to shorten data acquisition time and reduce data redundancy. To demonstrate the potential of our system in deciphering the relationship of the multiple components of neural circuits, we acquired and quantified the brain-wide distributions of cholinergic neurons and input of ventral Caudoputamen (CP) with the anatomical annotation in the same brain. We further identified the cholinergic type of upstream neurons projecting to CP through the triple-color collocated analysis and quantified its proportions in the two brain-wide distributions. Both accounted for 0.22%, implying CP might be modulated by non-cholinergic neurons. Our method provides a new research tool for studying the different biological components in the same organ and potentially facilitates the understanding of the processing mechanism of neural circuits and other biological activities.
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spelling pubmed-95838162022-10-21 Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain Ding, Zhangheng Zhao, Jiangjiang Luo, Tianpeng Lu, Bolin Zhang, Xiaoyu Chen, Siqi Li, Anan Jia, Xueyan Zhang, Jianmin Chen, Wu Chen, Jianwei Sun, Qingtao Li, Xiangning Gong, Hui Yuan, Jing Front Neurosci Neuroscience Visualizing the relationships and interactions among different biological components in the whole brain is crucial to our understanding of brain structures and functions. However, an automatic multicolor whole-brain imaging technique is still lacking. Here, we developed a multicolor wide-field large-volume tomography (multicolor WVT) to simultaneously acquire fluorescent signals in blue, green, and red channels in the whole brain. To facilitate the segmentation of brain regions and anatomical annotation, we used 4′, 6-diamidino-2-phenylindole (DAPI) to provide cytoarchitecture through real-time counterstaining. We optimized the imaging planes and modes of three channels to overcome the axial chromatic aberration of the illumination path and avoid the crosstalk from DAPI to the green channel without the modification of system configuration. We also developed an automatic contour recognition algorithm based on DAPI-staining cytoarchitecture to shorten data acquisition time and reduce data redundancy. To demonstrate the potential of our system in deciphering the relationship of the multiple components of neural circuits, we acquired and quantified the brain-wide distributions of cholinergic neurons and input of ventral Caudoputamen (CP) with the anatomical annotation in the same brain. We further identified the cholinergic type of upstream neurons projecting to CP through the triple-color collocated analysis and quantified its proportions in the two brain-wide distributions. Both accounted for 0.22%, implying CP might be modulated by non-cholinergic neurons. Our method provides a new research tool for studying the different biological components in the same organ and potentially facilitates the understanding of the processing mechanism of neural circuits and other biological activities. Frontiers Media S.A. 2022-10-06 /pmc/articles/PMC9583816/ /pubmed/36278018 http://dx.doi.org/10.3389/fnins.2022.1033880 Text en Copyright © 2022 Ding, Zhao, Luo, Lu, Zhang, Chen, Li, Jia, Zhang, Chen, Chen, Sun, Li, Gong and Yuan. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Neuroscience
Ding, Zhangheng
Zhao, Jiangjiang
Luo, Tianpeng
Lu, Bolin
Zhang, Xiaoyu
Chen, Siqi
Li, Anan
Jia, Xueyan
Zhang, Jianmin
Chen, Wu
Chen, Jianwei
Sun, Qingtao
Li, Xiangning
Gong, Hui
Yuan, Jing
Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
title Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
title_full Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
title_fullStr Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
title_full_unstemmed Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
title_short Multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
title_sort multicolor high-resolution whole-brain imaging for acquiring and comparing the brain-wide distributions of type-specific and projection-specific neurons with anatomical annotation in the same brain
topic Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9583816/
https://www.ncbi.nlm.nih.gov/pubmed/36278018
http://dx.doi.org/10.3389/fnins.2022.1033880
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