CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus

This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-)...

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Detalles Bibliográficos
Autores principales: Zhou, Hongyu, Bu, Shengjun, Xu, Yao, Xue, Lulu, Li, Zhongyi, Hao, Zhuo, Wan, Jiayu, Tang, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2022
Materias:
Research Paper
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584272/
https://www.ncbi.nlm.nih.gov/pubmed/36264297
http://dx.doi.org/10.1007/s00216-022-04380-1
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author Zhou, Hongyu
Bu, Shengjun
Xu, Yao
Xue, Lulu
Li, Zhongyi
Hao, Zhuo
Wan, Jiayu
Tang, Feng
author_facet Zhou, Hongyu
Bu, Shengjun
Xu, Yao
Xue, Lulu
Li, Zhongyi
Hao, Zhuo
Wan, Jiayu
Tang, Feng
author_sort Zhou, Hongyu
collection PubMed
description This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-) of the probe, further initiating HCR to copiously generate G-rich DNA. Abundant G-quadruplex/hemin was formed in the presence of hemin, thus catalyzing a colorimetric reaction. The colorimetric biosensor exhibited a linear relationship from 10 pM to 100 nM. The detection limit was 0.152 pM. The biosensor specificity was excellent. This new and sensitive detection method for influenza virus is a promising rapid influenza diagnostic test. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-04380-1.
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spelling pubmed-95842722022-10-21 CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus Zhou, Hongyu Bu, Shengjun Xu, Yao Xue, Lulu Li, Zhongyi Hao, Zhuo Wan, Jiayu Tang, Feng Anal Bioanal Chem Research Paper This study provides proof of concept of a colorimetric biosensor for influenza H1N1 virus assay based on the CRISPR/Cas13a system and hybridization chain reaction (HCR). Target RNA of influenza H1N1 virus activated the trans-cleavage activity of Cas13a, which cleaved the special RNA sequence (-UUU-) of the probe, further initiating HCR to copiously generate G-rich DNA. Abundant G-quadruplex/hemin was formed in the presence of hemin, thus catalyzing a colorimetric reaction. The colorimetric biosensor exhibited a linear relationship from 10 pM to 100 nM. The detection limit was 0.152 pM. The biosensor specificity was excellent. This new and sensitive detection method for influenza virus is a promising rapid influenza diagnostic test. GRAPHICAL ABSTRACT: [Image: see text] SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00216-022-04380-1. Springer Berlin Heidelberg 2022-10-20 2022 /pmc/articles/PMC9584272/ /pubmed/36264297 http://dx.doi.org/10.1007/s00216-022-04380-1 Text en © Springer-Verlag GmbH Germany, part of Springer Nature 2022, Springer Nature or its licensor (e.g. a society or other partner) holds exclusive rights to this article under a publishing agreement with the author(s) or other rightsholder(s); author self-archiving of the accepted manuscript version of this article is solely governed by the terms of such publishing agreement and applicable law. This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.
spellingShingle Research Paper
Zhou, Hongyu
Bu, Shengjun
Xu, Yao
Xue, Lulu
Li, Zhongyi
Hao, Zhuo
Wan, Jiayu
Tang, Feng
CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
title CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
title_full CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
title_fullStr CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
title_full_unstemmed CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
title_short CRISPR/Cas13a combined with hybridization chain reaction for visual detection of influenza A (H1N1) virus
title_sort crispr/cas13a combined with hybridization chain reaction for visual detection of influenza a (h1n1) virus
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584272/
https://www.ncbi.nlm.nih.gov/pubmed/36264297
http://dx.doi.org/10.1007/s00216-022-04380-1
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