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Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns

The effects of mother-infant separation (i.e., weaning) on the physiology, psychology and nutrition of mammalian infants have attracted much attention. Forest musk deer (FMD) is a first-class protected species in China and listed endangered in the IUCN Red List. The captive breeding population is no...

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Autores principales: Li, Yimeng, Shi, Minghui, Zhang, Baofeng, Wu, Jiahui, Wang, Yichen, Li, Mengqi, Wu, Yining, Hu, Xin, Hu, Defu, Huang, Zhixin, Wronski, Torsten
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584425/
https://www.ncbi.nlm.nih.gov/pubmed/36264997
http://dx.doi.org/10.1371/journal.pone.0276542
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author Li, Yimeng
Shi, Minghui
Zhang, Baofeng
Wu, Jiahui
Wang, Yichen
Li, Mengqi
Wu, Yining
Hu, Xin
Hu, Defu
Huang, Zhixin
Wronski, Torsten
author_facet Li, Yimeng
Shi, Minghui
Zhang, Baofeng
Wu, Jiahui
Wang, Yichen
Li, Mengqi
Wu, Yining
Hu, Xin
Hu, Defu
Huang, Zhixin
Wronski, Torsten
author_sort Li, Yimeng
collection PubMed
description The effects of mother-infant separation (i.e., weaning) on the physiology, psychology and nutrition of mammalian infants have attracted much attention. Forest musk deer (FMD) is a first-class protected species in China and listed endangered in the IUCN Red List. The captive breeding population is not only an important source for restocking of wild resources, but also a necessary way to supply the market with legal musk. So far, there is no scientific basis for the appropriate separation time of FMD females and their infants. Therefore, we used metagenome sequencing and enzyme-linked immunosorbent assays to study changes in the fecal cortisol concentration, as well as the intestinal microbiome composition and function of females and fawns at three different separation times, i.e., after 80 days, 90 days and 100 days. The results showed that the increment of the cortisol concentration in female FMD increased with increasing lactation time. The increment of cortisol concentration in infant FMD was highest in the 80 days weaning group, but there was no significant difference between the 90 days and the 100 days separation time. Based on the annotation results of COG, KEGG and CAZy databases, the abundance of different functions annotated by the intestinal microbiome of mothers and fawns of the 90 days weaning group changed slightly after separation. Based on the above results, the separation of mother and infant FMD is recommended after 90 days, i.e., the separation time that triggered the lowest rate of weaning stress and that supported a relatively stable gastro-intestinal physiology.
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spelling pubmed-95844252022-10-21 Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns Li, Yimeng Shi, Minghui Zhang, Baofeng Wu, Jiahui Wang, Yichen Li, Mengqi Wu, Yining Hu, Xin Hu, Defu Huang, Zhixin Wronski, Torsten PLoS One Research Article The effects of mother-infant separation (i.e., weaning) on the physiology, psychology and nutrition of mammalian infants have attracted much attention. Forest musk deer (FMD) is a first-class protected species in China and listed endangered in the IUCN Red List. The captive breeding population is not only an important source for restocking of wild resources, but also a necessary way to supply the market with legal musk. So far, there is no scientific basis for the appropriate separation time of FMD females and their infants. Therefore, we used metagenome sequencing and enzyme-linked immunosorbent assays to study changes in the fecal cortisol concentration, as well as the intestinal microbiome composition and function of females and fawns at three different separation times, i.e., after 80 days, 90 days and 100 days. The results showed that the increment of the cortisol concentration in female FMD increased with increasing lactation time. The increment of cortisol concentration in infant FMD was highest in the 80 days weaning group, but there was no significant difference between the 90 days and the 100 days separation time. Based on the annotation results of COG, KEGG and CAZy databases, the abundance of different functions annotated by the intestinal microbiome of mothers and fawns of the 90 days weaning group changed slightly after separation. Based on the above results, the separation of mother and infant FMD is recommended after 90 days, i.e., the separation time that triggered the lowest rate of weaning stress and that supported a relatively stable gastro-intestinal physiology. Public Library of Science 2022-10-20 /pmc/articles/PMC9584425/ /pubmed/36264997 http://dx.doi.org/10.1371/journal.pone.0276542 Text en © 2022 Li et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Li, Yimeng
Shi, Minghui
Zhang, Baofeng
Wu, Jiahui
Wang, Yichen
Li, Mengqi
Wu, Yining
Hu, Xin
Hu, Defu
Huang, Zhixin
Wronski, Torsten
Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns
title Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns
title_full Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns
title_fullStr Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns
title_full_unstemmed Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns
title_short Effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (Moschus berezovskii) and their fawns
title_sort effects of different weaning times on the stress response and the intestinal microbiota composition of female forest musk deer (moschus berezovskii) and their fawns
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584425/
https://www.ncbi.nlm.nih.gov/pubmed/36264997
http://dx.doi.org/10.1371/journal.pone.0276542
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