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Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury
OBJECTIVE: This paper aims to explore novel ferroptosis-related biomarkers for acute kidney injury (AKI). METHODS: Various bioinformatic methods, such as differential expression analysis, functional annotation analysis, machine learning, and chemical-gene network analysis, were used in this study. F...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584673/ https://www.ncbi.nlm.nih.gov/pubmed/36277893 http://dx.doi.org/10.1155/2022/3667339 |
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author | Zhang, Hao Liu, Xuemei Zhou, Lizhi Deng, Zebin Wang, Yinhuai |
author_facet | Zhang, Hao Liu, Xuemei Zhou, Lizhi Deng, Zebin Wang, Yinhuai |
author_sort | Zhang, Hao |
collection | PubMed |
description | OBJECTIVE: This paper aims to explore novel ferroptosis-related biomarkers for acute kidney injury (AKI). METHODS: Various bioinformatic methods, such as differential expression analysis, functional annotation analysis, machine learning, and chemical-gene network analysis, were used in this study. Furthermore, the expression and proferroptotic role of RPS7 were validated with further bioinformatics analysis and biochemical experiments. RESULTS: GSE30718 dataset and GSE139061 dataset were used, and the differentially expressed genes (DEGs) were screened. The DEGs were overlapped with ferroptosis-related genes and genes associated with AKI, which led to the identification of four candidate genes. Machine learning and ROC curve analysis were conducted, and RPS7 and TRIB3 were selected for diagnostic model analysis and functional analysis. Finally, the upregulation of RSP7 in cisplatin-induced AKI was validated in cisplatin-induced AKI, and its proferroptotic role was confirmed in cisplatin-treated proximal tubular cells. CONCLUSION: Our results indicated that RPS7 might present as a novel ferroptosis-related biomarker for AKI, and it derived ferroptosis to accentuate cisplatin-induced AKI. |
format | Online Article Text |
id | pubmed-9584673 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-95846732022-10-21 Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury Zhang, Hao Liu, Xuemei Zhou, Lizhi Deng, Zebin Wang, Yinhuai Biomed Res Int Research Article OBJECTIVE: This paper aims to explore novel ferroptosis-related biomarkers for acute kidney injury (AKI). METHODS: Various bioinformatic methods, such as differential expression analysis, functional annotation analysis, machine learning, and chemical-gene network analysis, were used in this study. Furthermore, the expression and proferroptotic role of RPS7 were validated with further bioinformatics analysis and biochemical experiments. RESULTS: GSE30718 dataset and GSE139061 dataset were used, and the differentially expressed genes (DEGs) were screened. The DEGs were overlapped with ferroptosis-related genes and genes associated with AKI, which led to the identification of four candidate genes. Machine learning and ROC curve analysis were conducted, and RPS7 and TRIB3 were selected for diagnostic model analysis and functional analysis. Finally, the upregulation of RSP7 in cisplatin-induced AKI was validated in cisplatin-induced AKI, and its proferroptotic role was confirmed in cisplatin-treated proximal tubular cells. CONCLUSION: Our results indicated that RPS7 might present as a novel ferroptosis-related biomarker for AKI, and it derived ferroptosis to accentuate cisplatin-induced AKI. Hindawi 2022-10-13 /pmc/articles/PMC9584673/ /pubmed/36277893 http://dx.doi.org/10.1155/2022/3667339 Text en Copyright © 2022 Hao Zhang et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Zhang, Hao Liu, Xuemei Zhou, Lizhi Deng, Zebin Wang, Yinhuai Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury |
title | Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury |
title_full | Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury |
title_fullStr | Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury |
title_full_unstemmed | Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury |
title_short | Identification of RPS7 as the Biomarker of Ferroptosis in Acute Kidney Injury |
title_sort | identification of rps7 as the biomarker of ferroptosis in acute kidney injury |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9584673/ https://www.ncbi.nlm.nih.gov/pubmed/36277893 http://dx.doi.org/10.1155/2022/3667339 |
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