ROS-Generating Poly(Ethylene Glycol)-Conjugated Fe(3)O(4) Nanoparticles as Cancer-Targeting Sustained Release Carrier of Doxorubicin

PURPOSE: Site-specific drug delivery systems can contribute to the development and execution of effective cancer treatment. Due to its favorable features (including high biocompatibility, high hydrophilicity and ease of functionalization), poly(ethylene glycol) (PEG) has been widely adopted to design drug carriers. Generating carriers for delivery of hydrophobic anticancer agents, however, is still a challenge in carrier design. METHODS: In the first step, PEG is functionalized with dialdehyde to generate PEG-(CHO)(2) using EDC/NHS chemistry. In the second step, Fe(3)O(4) nanoparticles are functionalized with amino groups to generate Fe(3)O(4)-NH(2). In the third step, PEG-(CHO)(2), Fe(3)O(4)-NH(2) and doxorubicin (DOX) react in an acidic environment to yield a drug conjugate (PEGDA-MN-DOX), which is subsequently characterized by FT-IR, (1)H-NMR, SEM, TEM, DLS, TGA, and DSC. RESULTS: The chemical functionalities of the drug conjugate are confirmed by FTIR, H-NMRand XRD analysis.The release pattern of PEGDA-MN-DOX is investigated at 25 and 37 °C at different pH values. The results indicate that the developed drug conjugate cannot only behave as a sustained-release carrier, but can also generate a significant level of reactive oxygen species (ROS), leading to a high level of toxicity against MCF-7 cells while still showing excellent biocompatibility in 3T3 cells. CONCLUSION: The reported conjugate shows anticancer potential, cancer-targeting ability, and ROS-generating capacity for effective drug encapsulation and sustained release in chemotherapy.