Cargando…
Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium
Quinella is a genus of iconic rumen bacteria first reported in 1913. There are no cultures of these bacteria, and information on their physiology is scarce and contradictory. Increased abundance of Quinella was previously found in the rumens of some sheep that emit low amounts of methane (CH(4)) rel...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585023/ https://www.ncbi.nlm.nih.gov/pubmed/36266280 http://dx.doi.org/10.1038/s41467-022-34013-1 |
_version_ | 1784813406563336192 |
---|---|
author | Kumar, Sandeep Altermann, Eric Leahy, Sinead C. Jauregui, Ruy Jonker, Arjan Henderson, Gemma Kittelmann, Sandra Attwood, Graeme T. Kamke, Janine Waters, Sinéad M. Patchett, Mark L. Janssen, Peter H. |
author_facet | Kumar, Sandeep Altermann, Eric Leahy, Sinead C. Jauregui, Ruy Jonker, Arjan Henderson, Gemma Kittelmann, Sandra Attwood, Graeme T. Kamke, Janine Waters, Sinéad M. Patchett, Mark L. Janssen, Peter H. |
author_sort | Kumar, Sandeep |
collection | PubMed |
description | Quinella is a genus of iconic rumen bacteria first reported in 1913. There are no cultures of these bacteria, and information on their physiology is scarce and contradictory. Increased abundance of Quinella was previously found in the rumens of some sheep that emit low amounts of methane (CH(4)) relative to their feed intake, but whether Quinella contributes to low CH(4) emissions is not known. Here, we concentrate Quinella cells from sheep rumen contents, extract and sequence DNA, and reconstruct Quinella genomes that are >90% complete with as little as 0.20% contamination. Bioinformatic analyses of the encoded proteins indicate that lactate and propionate formation are major fermentation pathways. The presence of a gene encoding a potential uptake hydrogenase suggests that Quinella might be able to use free hydrogen (H(2)). None of the inferred metabolic pathways is predicted to produce H(2), a major precursor of CH(4), which is consistent with the lower CH(4) emissions from those sheep with high abundances of this bacterium. |
format | Online Article Text |
id | pubmed-9585023 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-95850232022-10-22 Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium Kumar, Sandeep Altermann, Eric Leahy, Sinead C. Jauregui, Ruy Jonker, Arjan Henderson, Gemma Kittelmann, Sandra Attwood, Graeme T. Kamke, Janine Waters, Sinéad M. Patchett, Mark L. Janssen, Peter H. Nat Commun Article Quinella is a genus of iconic rumen bacteria first reported in 1913. There are no cultures of these bacteria, and information on their physiology is scarce and contradictory. Increased abundance of Quinella was previously found in the rumens of some sheep that emit low amounts of methane (CH(4)) relative to their feed intake, but whether Quinella contributes to low CH(4) emissions is not known. Here, we concentrate Quinella cells from sheep rumen contents, extract and sequence DNA, and reconstruct Quinella genomes that are >90% complete with as little as 0.20% contamination. Bioinformatic analyses of the encoded proteins indicate that lactate and propionate formation are major fermentation pathways. The presence of a gene encoding a potential uptake hydrogenase suggests that Quinella might be able to use free hydrogen (H(2)). None of the inferred metabolic pathways is predicted to produce H(2), a major precursor of CH(4), which is consistent with the lower CH(4) emissions from those sheep with high abundances of this bacterium. Nature Publishing Group UK 2022-10-20 /pmc/articles/PMC9585023/ /pubmed/36266280 http://dx.doi.org/10.1038/s41467-022-34013-1 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Kumar, Sandeep Altermann, Eric Leahy, Sinead C. Jauregui, Ruy Jonker, Arjan Henderson, Gemma Kittelmann, Sandra Attwood, Graeme T. Kamke, Janine Waters, Sinéad M. Patchett, Mark L. Janssen, Peter H. Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium |
title | Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium |
title_full | Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium |
title_fullStr | Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium |
title_full_unstemmed | Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium |
title_short | Genomic insights into the physiology of Quinella, an iconic uncultured rumen bacterium |
title_sort | genomic insights into the physiology of quinella, an iconic uncultured rumen bacterium |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585023/ https://www.ncbi.nlm.nih.gov/pubmed/36266280 http://dx.doi.org/10.1038/s41467-022-34013-1 |
work_keys_str_mv | AT kumarsandeep genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT altermanneric genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT leahysineadc genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT jaureguiruy genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT jonkerarjan genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT hendersongemma genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT kittelmannsandra genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT attwoodgraemet genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT kamkejanine genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT waterssineadm genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT patchettmarkl genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium AT janssenpeterh genomicinsightsintothephysiologyofquinellaaniconicunculturedrumenbacterium |