Recent advances in PCR-free nucleic acid detection for SARS-COV-2

As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold...

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Autores principales: Ma, Xiaowei, Xu, Jingsong, Zhou, Fei, Ye, Jing, Yang, Donglei, Wang, Hua, Wang, Pengfei, Li, Min
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Bioengineering and Biotechnology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585218/
https://www.ncbi.nlm.nih.gov/pubmed/36277389
http://dx.doi.org/10.3389/fbioe.2022.999358
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author Ma, Xiaowei
Xu, Jingsong
Zhou, Fei
Ye, Jing
Yang, Donglei
Wang, Hua
Wang, Pengfei
Li, Min
author_facet Ma, Xiaowei
Xu, Jingsong
Zhou, Fei
Ye, Jing
Yang, Donglei
Wang, Hua
Wang, Pengfei
Li, Min
author_sort Ma, Xiaowei
collection PubMed
description As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR.
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spelling pubmed-95852182022-10-22 Recent advances in PCR-free nucleic acid detection for SARS-COV-2 Ma, Xiaowei Xu, Jingsong Zhou, Fei Ye, Jing Yang, Donglei Wang, Hua Wang, Pengfei Li, Min Front Bioeng Biotechnol Bioengineering and Biotechnology As the outbreak of Coronavirus disease 2019 (COVID-19) caused by severe acute respiratory disease coronavirus 2 (SARS-COV-2), fast, accurate, and economic detection of viral infection has become crucial for stopping the spread. Polymerase chain reaction (PCR) of viral nucleic acids has been the gold standard method for SARS-COV-2 detection, which, however, generally requires sophisticated facilities and laboratory space, and is time consuming. This review presents recent advances in PCR-free nucleic acid detection methods for SARS-CoV-2, including emerging methods of isothermal amplification, nucleic acid enzymes, electrochemistry and CRISPR. Frontiers Media S.A. 2022-10-07 /pmc/articles/PMC9585218/ /pubmed/36277389 http://dx.doi.org/10.3389/fbioe.2022.999358 Text en Copyright © 2022 Ma, Xu, Zhou, Ye, Yang, Wang, Wang and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Ma, Xiaowei
Xu, Jingsong
Zhou, Fei
Ye, Jing
Yang, Donglei
Wang, Hua
Wang, Pengfei
Li, Min
Recent advances in PCR-free nucleic acid detection for SARS-COV-2
title Recent advances in PCR-free nucleic acid detection for SARS-COV-2
title_full Recent advances in PCR-free nucleic acid detection for SARS-COV-2
title_fullStr Recent advances in PCR-free nucleic acid detection for SARS-COV-2
title_full_unstemmed Recent advances in PCR-free nucleic acid detection for SARS-COV-2
title_short Recent advances in PCR-free nucleic acid detection for SARS-COV-2
title_sort recent advances in pcr-free nucleic acid detection for sars-cov-2
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585218/
https://www.ncbi.nlm.nih.gov/pubmed/36277389
http://dx.doi.org/10.3389/fbioe.2022.999358
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