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Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa

We designed and synthesized recombinant phage nanofibers displaying ToAP2D peptide and investigated their antifungal effect on Sporothrix and the corresponding mechanism. Antimicrobial peptide, ToAP2D, was used as the template. The effect of synthesized recombinant phages on the immune function of C...

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Autores principales: Yan, Tianyi, An, Lin, Chen, Feng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585229/
https://www.ncbi.nlm.nih.gov/pubmed/36278206
http://dx.doi.org/10.3389/fphar.2022.1022651
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author Yan, Tianyi
An, Lin
Chen, Feng
author_facet Yan, Tianyi
An, Lin
Chen, Feng
author_sort Yan, Tianyi
collection PubMed
description We designed and synthesized recombinant phage nanofibers displaying ToAP2D peptide and investigated their antifungal effect on Sporothrix and the corresponding mechanism. Antimicrobial peptide, ToAP2D, was used as the template. The effect of synthesized recombinant phages on the immune function of CD4(+) T lymphocytes in mice was tested using an enzyme-linked immunosorbent assay. The therapeutic effect and safety of recombinant phage administration on Sporothrix-infected BALB/c mice were evaluated based on survival analysis, histopathological changes, and renal and liver functions. The successfully prepared recombinant phage displaying ToAP2D peptides significantly inhibited Sporothrix growth. According to the scanning electron microscopy results, the recombinant phage caused shrinkage and rupture of Sporothrix globosa, leading to leakage of the contents. The Hoechst/propidium iodide double staining test indicated that the recombinant phage could induce cell apoptosis of Sporothrix globosa. The apoptotic pathway might be due to the accumulation of reactive oxygen species in large quantities in cells, activating caspase dependence; this reduced inflammation, prolonged the survival time, and enhanced levels of IFN-γ and IL-17 in mice. We believe that recombinant phage inhibits Sporothrix growth by adjusting the immune response of mice, inducing Sporothrix apoptosis and improving animal survival. This study offers a new approach to preparing antimicrobial peptides.
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spelling pubmed-95852292022-10-22 Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa Yan, Tianyi An, Lin Chen, Feng Front Pharmacol Pharmacology We designed and synthesized recombinant phage nanofibers displaying ToAP2D peptide and investigated their antifungal effect on Sporothrix and the corresponding mechanism. Antimicrobial peptide, ToAP2D, was used as the template. The effect of synthesized recombinant phages on the immune function of CD4(+) T lymphocytes in mice was tested using an enzyme-linked immunosorbent assay. The therapeutic effect and safety of recombinant phage administration on Sporothrix-infected BALB/c mice were evaluated based on survival analysis, histopathological changes, and renal and liver functions. The successfully prepared recombinant phage displaying ToAP2D peptides significantly inhibited Sporothrix growth. According to the scanning electron microscopy results, the recombinant phage caused shrinkage and rupture of Sporothrix globosa, leading to leakage of the contents. The Hoechst/propidium iodide double staining test indicated that the recombinant phage could induce cell apoptosis of Sporothrix globosa. The apoptotic pathway might be due to the accumulation of reactive oxygen species in large quantities in cells, activating caspase dependence; this reduced inflammation, prolonged the survival time, and enhanced levels of IFN-γ and IL-17 in mice. We believe that recombinant phage inhibits Sporothrix growth by adjusting the immune response of mice, inducing Sporothrix apoptosis and improving animal survival. This study offers a new approach to preparing antimicrobial peptides. Frontiers Media S.A. 2022-10-07 /pmc/articles/PMC9585229/ /pubmed/36278206 http://dx.doi.org/10.3389/fphar.2022.1022651 Text en Copyright © 2022 Yan, An and Chen. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Pharmacology
Yan, Tianyi
An, Lin
Chen, Feng
Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa
title Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa
title_full Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa
title_fullStr Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa
title_full_unstemmed Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa
title_short Recombinant phage displaying ToAP2D peptide with antifungal activity against Sporothrix globosa
title_sort recombinant phage displaying toap2d peptide with antifungal activity against sporothrix globosa
topic Pharmacology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585229/
https://www.ncbi.nlm.nih.gov/pubmed/36278206
http://dx.doi.org/10.3389/fphar.2022.1022651
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