Loss of Cep72 affects the morphology of spermatozoa in mice

The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identif...

Descripción completa

Detalles Bibliográficos
Autores principales: Chen, Zhen, Xu, Yating, Ma, Dupeng, Li, Changrong, Yu, Ziqi, Liu, Cong, Jin, Tingyu, Du, Ziye, Li, Zejia, Sun, Qi, Xu, Yumin, Liu, Rong, Wu, Yuerong, Luo, Mengcheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Physiology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585255/
https://www.ncbi.nlm.nih.gov/pubmed/36277211
http://dx.doi.org/10.3389/fphys.2022.948965
_version_ 1784813451710824448
author Chen, Zhen
Xu, Yating
Ma, Dupeng
Li, Changrong
Yu, Ziqi
Liu, Cong
Jin, Tingyu
Du, Ziye
Li, Zejia
Sun, Qi
Xu, Yumin
Liu, Rong
Wu, Yuerong
Luo, Mengcheng
author_facet Chen, Zhen
Xu, Yating
Ma, Dupeng
Li, Changrong
Yu, Ziqi
Liu, Cong
Jin, Tingyu
Du, Ziye
Li, Zejia
Sun, Qi
Xu, Yumin
Liu, Rong
Wu, Yuerong
Luo, Mengcheng
author_sort Chen, Zhen
collection PubMed
description The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identify Cep72 as a testis-specific expression protein. Although Cep72 knockout mice were viable and fertile, their sperms were morphologically abnormal with incomplete flagellum structures. Transcriptome analysis reveals significant differences in six genes (Gm49527, Hbb-bt, Hba-a2, Rps27a-ps2, Gm29647, and Gm8430), which were not previously associated with spermatogenesis. Overall, these results indicate that Cep72 participates in regulating sperm morphology and yet is dispensable for fertility in mice.
format Online
Article
Text
id pubmed-9585255
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-95852552022-10-22 Loss of Cep72 affects the morphology of spermatozoa in mice Chen, Zhen Xu, Yating Ma, Dupeng Li, Changrong Yu, Ziqi Liu, Cong Jin, Tingyu Du, Ziye Li, Zejia Sun, Qi Xu, Yumin Liu, Rong Wu, Yuerong Luo, Mengcheng Front Physiol Physiology The centrosome regulates mammalian meiosis by affecting recombination, synapsis, chromosome segregation, and spermiogenesis. Cep72 is one of the critical components of the centrosome. However, the physiological role of Cep72 in spermatogenesis and fertility remains unclear. In this study, we identify Cep72 as a testis-specific expression protein. Although Cep72 knockout mice were viable and fertile, their sperms were morphologically abnormal with incomplete flagellum structures. Transcriptome analysis reveals significant differences in six genes (Gm49527, Hbb-bt, Hba-a2, Rps27a-ps2, Gm29647, and Gm8430), which were not previously associated with spermatogenesis. Overall, these results indicate that Cep72 participates in regulating sperm morphology and yet is dispensable for fertility in mice. Frontiers Media S.A. 2022-10-07 /pmc/articles/PMC9585255/ /pubmed/36277211 http://dx.doi.org/10.3389/fphys.2022.948965 Text en Copyright © 2022 Chen, Xu, Ma, Li, Yu, Liu, Jin, Du, Li, Sun, Xu, Liu, Wu and Luo. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chen, Zhen
Xu, Yating
Ma, Dupeng
Li, Changrong
Yu, Ziqi
Liu, Cong
Jin, Tingyu
Du, Ziye
Li, Zejia
Sun, Qi
Xu, Yumin
Liu, Rong
Wu, Yuerong
Luo, Mengcheng
Loss of Cep72 affects the morphology of spermatozoa in mice
title Loss of Cep72 affects the morphology of spermatozoa in mice
title_full Loss of Cep72 affects the morphology of spermatozoa in mice
title_fullStr Loss of Cep72 affects the morphology of spermatozoa in mice
title_full_unstemmed Loss of Cep72 affects the morphology of spermatozoa in mice
title_short Loss of Cep72 affects the morphology of spermatozoa in mice
title_sort loss of cep72 affects the morphology of spermatozoa in mice
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585255/
https://www.ncbi.nlm.nih.gov/pubmed/36277211
http://dx.doi.org/10.3389/fphys.2022.948965
work_keys_str_mv AT chenzhen lossofcep72affectsthemorphologyofspermatozoainmice
AT xuyating lossofcep72affectsthemorphologyofspermatozoainmice
AT madupeng lossofcep72affectsthemorphologyofspermatozoainmice
AT lichangrong lossofcep72affectsthemorphologyofspermatozoainmice
AT yuziqi lossofcep72affectsthemorphologyofspermatozoainmice
AT liucong lossofcep72affectsthemorphologyofspermatozoainmice
AT jintingyu lossofcep72affectsthemorphologyofspermatozoainmice
AT duziye lossofcep72affectsthemorphologyofspermatozoainmice
AT lizejia lossofcep72affectsthemorphologyofspermatozoainmice
AT sunqi lossofcep72affectsthemorphologyofspermatozoainmice
AT xuyumin lossofcep72affectsthemorphologyofspermatozoainmice
AT liurong lossofcep72affectsthemorphologyofspermatozoainmice
AT wuyuerong lossofcep72affectsthemorphologyofspermatozoainmice
AT luomengcheng lossofcep72affectsthemorphologyofspermatozoainmice

Ejemplares similares