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Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
[Image: see text] Poison frogs are well-known for their fascinating ability to store alkaloids in their skin as chemical defense against predators. Chemical methods used to study these alkaloids are limited by requirements for euthanasia or stress during sampling. Here, we demonstrate sensitive and...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585640/ https://www.ncbi.nlm.nih.gov/pubmed/36281295 http://dx.doi.org/10.1021/acsmeasuresciau.2c00035 |
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author | Krieger, Anna C. Povilaitis, Sydney C. Gowda, Prajwal O’Connell, Lauren A. Eberlin, Livia S. |
author_facet | Krieger, Anna C. Povilaitis, Sydney C. Gowda, Prajwal O’Connell, Lauren A. Eberlin, Livia S. |
author_sort | Krieger, Anna C. |
collection | PubMed |
description | [Image: see text] Poison frogs are well-known for their fascinating ability to store alkaloids in their skin as chemical defense against predators. Chemical methods used to study these alkaloids are limited by requirements for euthanasia or stress during sampling. Here, we demonstrate sensitive and biocompatible alkaloid detection and monitoring in vivo using the MasSpec Pen, a handheld, noninvasive chemical detection device coupled to a mass spectrometer. The MasSpec Pen allowed rapid (<15 s), gentle, and consecutive molecular analysis without harm or undue stress to the animals. Through a month-long alkaloid-feeding study with the dyeing poison frog, we observed temporal dynamics of chemical sequestration in vivo by comparing frogs fed the alkaloid decahydroquinoline (DHQ) to vehicle-fed frogs. We also demonstrate the feasibility of the MasSpec Pen for the untargeted detection of rich alkaloid profiles from skin extracts of the Diablito poison frog, collected from two distinct geographical populations in Ecuador. The results obtained in this study demonstrate the utility of the MasSpec Pen for direct, rapid, and biocompatible analysis of poison frog alkaloids. |
format | Online Article Text |
id | pubmed-9585640 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-95856402022-10-22 Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen Krieger, Anna C. Povilaitis, Sydney C. Gowda, Prajwal O’Connell, Lauren A. Eberlin, Livia S. ACS Meas Sci Au [Image: see text] Poison frogs are well-known for their fascinating ability to store alkaloids in their skin as chemical defense against predators. Chemical methods used to study these alkaloids are limited by requirements for euthanasia or stress during sampling. Here, we demonstrate sensitive and biocompatible alkaloid detection and monitoring in vivo using the MasSpec Pen, a handheld, noninvasive chemical detection device coupled to a mass spectrometer. The MasSpec Pen allowed rapid (<15 s), gentle, and consecutive molecular analysis without harm or undue stress to the animals. Through a month-long alkaloid-feeding study with the dyeing poison frog, we observed temporal dynamics of chemical sequestration in vivo by comparing frogs fed the alkaloid decahydroquinoline (DHQ) to vehicle-fed frogs. We also demonstrate the feasibility of the MasSpec Pen for the untargeted detection of rich alkaloid profiles from skin extracts of the Diablito poison frog, collected from two distinct geographical populations in Ecuador. The results obtained in this study demonstrate the utility of the MasSpec Pen for direct, rapid, and biocompatible analysis of poison frog alkaloids. American Chemical Society 2022-08-10 /pmc/articles/PMC9585640/ /pubmed/36281295 http://dx.doi.org/10.1021/acsmeasuresciau.2c00035 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Krieger, Anna C. Povilaitis, Sydney C. Gowda, Prajwal O’Connell, Lauren A. Eberlin, Livia S. Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen |
title | Noninvasive
Detection of Chemical Defenses in Poison
Frogs Using the MasSpec Pen |
title_full | Noninvasive
Detection of Chemical Defenses in Poison
Frogs Using the MasSpec Pen |
title_fullStr | Noninvasive
Detection of Chemical Defenses in Poison
Frogs Using the MasSpec Pen |
title_full_unstemmed | Noninvasive
Detection of Chemical Defenses in Poison
Frogs Using the MasSpec Pen |
title_short | Noninvasive
Detection of Chemical Defenses in Poison
Frogs Using the MasSpec Pen |
title_sort | noninvasive
detection of chemical defenses in poison
frogs using the masspec pen |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585640/ https://www.ncbi.nlm.nih.gov/pubmed/36281295 http://dx.doi.org/10.1021/acsmeasuresciau.2c00035 |
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