Cargando…

Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen

[Image: see text] Poison frogs are well-known for their fascinating ability to store alkaloids in their skin as chemical defense against predators. Chemical methods used to study these alkaloids are limited by requirements for euthanasia or stress during sampling. Here, we demonstrate sensitive and...

Descripción completa

Detalles Bibliográficos
Autores principales: Krieger, Anna C., Povilaitis, Sydney C., Gowda, Prajwal, O’Connell, Lauren A., Eberlin, Livia S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585640/
https://www.ncbi.nlm.nih.gov/pubmed/36281295
http://dx.doi.org/10.1021/acsmeasuresciau.2c00035
_version_ 1784813537012482048
author Krieger, Anna C.
Povilaitis, Sydney C.
Gowda, Prajwal
O’Connell, Lauren A.
Eberlin, Livia S.
author_facet Krieger, Anna C.
Povilaitis, Sydney C.
Gowda, Prajwal
O’Connell, Lauren A.
Eberlin, Livia S.
author_sort Krieger, Anna C.
collection PubMed
description [Image: see text] Poison frogs are well-known for their fascinating ability to store alkaloids in their skin as chemical defense against predators. Chemical methods used to study these alkaloids are limited by requirements for euthanasia or stress during sampling. Here, we demonstrate sensitive and biocompatible alkaloid detection and monitoring in vivo using the MasSpec Pen, a handheld, noninvasive chemical detection device coupled to a mass spectrometer. The MasSpec Pen allowed rapid (<15 s), gentle, and consecutive molecular analysis without harm or undue stress to the animals. Through a month-long alkaloid-feeding study with the dyeing poison frog, we observed temporal dynamics of chemical sequestration in vivo by comparing frogs fed the alkaloid decahydroquinoline (DHQ) to vehicle-fed frogs. We also demonstrate the feasibility of the MasSpec Pen for the untargeted detection of rich alkaloid profiles from skin extracts of the Diablito poison frog, collected from two distinct geographical populations in Ecuador. The results obtained in this study demonstrate the utility of the MasSpec Pen for direct, rapid, and biocompatible analysis of poison frog alkaloids.
format Online
Article
Text
id pubmed-9585640
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher American Chemical Society
record_format MEDLINE/PubMed
spelling pubmed-95856402022-10-22 Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen Krieger, Anna C. Povilaitis, Sydney C. Gowda, Prajwal O’Connell, Lauren A. Eberlin, Livia S. ACS Meas Sci Au [Image: see text] Poison frogs are well-known for their fascinating ability to store alkaloids in their skin as chemical defense against predators. Chemical methods used to study these alkaloids are limited by requirements for euthanasia or stress during sampling. Here, we demonstrate sensitive and biocompatible alkaloid detection and monitoring in vivo using the MasSpec Pen, a handheld, noninvasive chemical detection device coupled to a mass spectrometer. The MasSpec Pen allowed rapid (<15 s), gentle, and consecutive molecular analysis without harm or undue stress to the animals. Through a month-long alkaloid-feeding study with the dyeing poison frog, we observed temporal dynamics of chemical sequestration in vivo by comparing frogs fed the alkaloid decahydroquinoline (DHQ) to vehicle-fed frogs. We also demonstrate the feasibility of the MasSpec Pen for the untargeted detection of rich alkaloid profiles from skin extracts of the Diablito poison frog, collected from two distinct geographical populations in Ecuador. The results obtained in this study demonstrate the utility of the MasSpec Pen for direct, rapid, and biocompatible analysis of poison frog alkaloids. American Chemical Society 2022-08-10 /pmc/articles/PMC9585640/ /pubmed/36281295 http://dx.doi.org/10.1021/acsmeasuresciau.2c00035 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Krieger, Anna C.
Povilaitis, Sydney C.
Gowda, Prajwal
O’Connell, Lauren A.
Eberlin, Livia S.
Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
title Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
title_full Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
title_fullStr Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
title_full_unstemmed Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
title_short Noninvasive Detection of Chemical Defenses in Poison Frogs Using the MasSpec Pen
title_sort noninvasive detection of chemical defenses in poison frogs using the masspec pen
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585640/
https://www.ncbi.nlm.nih.gov/pubmed/36281295
http://dx.doi.org/10.1021/acsmeasuresciau.2c00035
work_keys_str_mv AT kriegerannac noninvasivedetectionofchemicaldefensesinpoisonfrogsusingthemasspecpen
AT povilaitissydneyc noninvasivedetectionofchemicaldefensesinpoisonfrogsusingthemasspecpen
AT gowdaprajwal noninvasivedetectionofchemicaldefensesinpoisonfrogsusingthemasspecpen
AT oconnelllaurena noninvasivedetectionofchemicaldefensesinpoisonfrogsusingthemasspecpen
AT eberlinlivias noninvasivedetectionofchemicaldefensesinpoisonfrogsusingthemasspecpen