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Plumbagin attenuates Bleomycin-induced lung fibrosis in mice
BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a fatal fibrotic lung disease with limited treatment options. Plumbagin (PL) is an herbal extract with diverse pharmacological effects that have been recently used to treat various types of cancer. This study aims to explore the anti-fibrotic effect...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585773/ https://www.ncbi.nlm.nih.gov/pubmed/36271442 http://dx.doi.org/10.1186/s13223-022-00734-7 |
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author | Mehdizadeh, Saber Taherian, Marjan Bayati, Paria Mousavizadeh, Kazem Pashangzadeh, Salar Anisian, Ali Mojtabavi, Nazanin |
author_facet | Mehdizadeh, Saber Taherian, Marjan Bayati, Paria Mousavizadeh, Kazem Pashangzadeh, Salar Anisian, Ali Mojtabavi, Nazanin |
author_sort | Mehdizadeh, Saber |
collection | PubMed |
description | BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a fatal fibrotic lung disease with limited treatment options. Plumbagin (PL) is an herbal extract with diverse pharmacological effects that have been recently used to treat various types of cancer. This study aims to explore the anti-fibrotic effect of PL and possible underlying mechanisms in IPF. METHODS: We used a bleomycin-induced experimental mouse model of lung fibrosis to assess the potential anti-fibrotic effect of PL. Histological analysis of lung tissue samples by H&E and Masson’s trichrome staining and hydroxyproline assay was performed to evaluate the fibrotic alterations. ELISA and real-time quantitative PCR were conducted to determine the amount of tumor necrosis factor-alpha (TNFα), tumor growth factor-beta (TGF-β), connective tissue growth factor (CTGF), and endothelin-1 (ET-1). RESULTS: Bleomycin exposure induced lung fibrosis, which was indicated by inflammation, collagen deposition, and structural damage. PL remarkably prevented bleomycin-induced lung fibrosis. Furthermore, PL significantly inhibited TNF-α and TGF-β production. PL also diminished the upregulated expression of CTGF and ET-1 induced by bleomycin. CONCLUSION: Overall, our findings suggest PL as an anti-fibrotic agent acting via down-regulation of TGF-β/CTGF or ET-1 axis, as well as TNF-α, to improve lung fibrosis. |
format | Online Article Text |
id | pubmed-9585773 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-95857732022-10-22 Plumbagin attenuates Bleomycin-induced lung fibrosis in mice Mehdizadeh, Saber Taherian, Marjan Bayati, Paria Mousavizadeh, Kazem Pashangzadeh, Salar Anisian, Ali Mojtabavi, Nazanin Allergy Asthma Clin Immunol Research BACKGROUND: Idiopathic pulmonary fibrosis (IPF) is a fatal fibrotic lung disease with limited treatment options. Plumbagin (PL) is an herbal extract with diverse pharmacological effects that have been recently used to treat various types of cancer. This study aims to explore the anti-fibrotic effect of PL and possible underlying mechanisms in IPF. METHODS: We used a bleomycin-induced experimental mouse model of lung fibrosis to assess the potential anti-fibrotic effect of PL. Histological analysis of lung tissue samples by H&E and Masson’s trichrome staining and hydroxyproline assay was performed to evaluate the fibrotic alterations. ELISA and real-time quantitative PCR were conducted to determine the amount of tumor necrosis factor-alpha (TNFα), tumor growth factor-beta (TGF-β), connective tissue growth factor (CTGF), and endothelin-1 (ET-1). RESULTS: Bleomycin exposure induced lung fibrosis, which was indicated by inflammation, collagen deposition, and structural damage. PL remarkably prevented bleomycin-induced lung fibrosis. Furthermore, PL significantly inhibited TNF-α and TGF-β production. PL also diminished the upregulated expression of CTGF and ET-1 induced by bleomycin. CONCLUSION: Overall, our findings suggest PL as an anti-fibrotic agent acting via down-regulation of TGF-β/CTGF or ET-1 axis, as well as TNF-α, to improve lung fibrosis. BioMed Central 2022-10-21 /pmc/articles/PMC9585773/ /pubmed/36271442 http://dx.doi.org/10.1186/s13223-022-00734-7 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Mehdizadeh, Saber Taherian, Marjan Bayati, Paria Mousavizadeh, Kazem Pashangzadeh, Salar Anisian, Ali Mojtabavi, Nazanin Plumbagin attenuates Bleomycin-induced lung fibrosis in mice |
title | Plumbagin attenuates Bleomycin-induced lung fibrosis in mice |
title_full | Plumbagin attenuates Bleomycin-induced lung fibrosis in mice |
title_fullStr | Plumbagin attenuates Bleomycin-induced lung fibrosis in mice |
title_full_unstemmed | Plumbagin attenuates Bleomycin-induced lung fibrosis in mice |
title_short | Plumbagin attenuates Bleomycin-induced lung fibrosis in mice |
title_sort | plumbagin attenuates bleomycin-induced lung fibrosis in mice |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9585773/ https://www.ncbi.nlm.nih.gov/pubmed/36271442 http://dx.doi.org/10.1186/s13223-022-00734-7 |
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