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Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods
Current computational technologies hold promise for prioritizing the testing of the thousands of chemicals in commerce. Here, a case study is presented demonstrating comparative risk-prioritization approaches based on the ratio of surrogate hazard and exposure data, called margins of exposure (MoEs)...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586287/ https://www.ncbi.nlm.nih.gov/pubmed/36278238 http://dx.doi.org/10.3389/fphar.2022.980747 |
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author | Nicolas, Chantel I. Linakis, Matthew W. Minto, Melyssa S. Mansouri, Kamel Clewell, Rebecca A. Yoon, Miyoung Wambaugh, John F. Patlewicz, Grace McMullen, Patrick D. Andersen, Melvin E. Clewell III, Harvey J. |
author_facet | Nicolas, Chantel I. Linakis, Matthew W. Minto, Melyssa S. Mansouri, Kamel Clewell, Rebecca A. Yoon, Miyoung Wambaugh, John F. Patlewicz, Grace McMullen, Patrick D. Andersen, Melvin E. Clewell III, Harvey J. |
author_sort | Nicolas, Chantel I. |
collection | PubMed |
description | Current computational technologies hold promise for prioritizing the testing of the thousands of chemicals in commerce. Here, a case study is presented demonstrating comparative risk-prioritization approaches based on the ratio of surrogate hazard and exposure data, called margins of exposure (MoEs). Exposures were estimated using a U.S. EPA’s ExpoCast predictive model (SEEM3) results and estimates of bioactivity were predicted using: 1) Oral equivalent doses (OEDs) derived from U.S. EPA’s ToxCast high-throughput screening program, together with in vitro to in vivo extrapolation and 2) thresholds of toxicological concern (TTCs) determined using a structure-based decision-tree using the Toxtree open source software. To ground-truth these computational approaches, we compared the MoEs based on predicted noncancer TTC and OED values to those derived using the traditional method of deriving points of departure from no-observed adverse effect levels (NOAELs) from in vivo oral exposures in rodents. TTC-based MoEs were lower than NOAEL-based MoEs for 520 out of 522 (99.6%) compounds in this smaller overlapping dataset, but were relatively well correlated with the same (r ( 2 ) = 0.59). TTC-based MoEs were also lower than OED-based MoEs for 590 (83.2%) of the 709 evaluated chemicals, indicating that TTCs may serve as a conservative surrogate in the absence of chemical-specific experimental data. The TTC-based MoE prioritization process was then applied to over 45,000 curated environmental chemical structures as a proof-of-concept for high-throughput prioritization using TTC-based MoEs. This study demonstrates the utility of exploiting existing computational methods at the pre-assessment phase of a tiered risk-based approach to quickly, and conservatively, prioritize thousands of untested chemicals for further study. |
format | Online Article Text |
id | pubmed-9586287 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-95862872022-10-22 Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods Nicolas, Chantel I. Linakis, Matthew W. Minto, Melyssa S. Mansouri, Kamel Clewell, Rebecca A. Yoon, Miyoung Wambaugh, John F. Patlewicz, Grace McMullen, Patrick D. Andersen, Melvin E. Clewell III, Harvey J. Front Pharmacol Pharmacology Current computational technologies hold promise for prioritizing the testing of the thousands of chemicals in commerce. Here, a case study is presented demonstrating comparative risk-prioritization approaches based on the ratio of surrogate hazard and exposure data, called margins of exposure (MoEs). Exposures were estimated using a U.S. EPA’s ExpoCast predictive model (SEEM3) results and estimates of bioactivity were predicted using: 1) Oral equivalent doses (OEDs) derived from U.S. EPA’s ToxCast high-throughput screening program, together with in vitro to in vivo extrapolation and 2) thresholds of toxicological concern (TTCs) determined using a structure-based decision-tree using the Toxtree open source software. To ground-truth these computational approaches, we compared the MoEs based on predicted noncancer TTC and OED values to those derived using the traditional method of deriving points of departure from no-observed adverse effect levels (NOAELs) from in vivo oral exposures in rodents. TTC-based MoEs were lower than NOAEL-based MoEs for 520 out of 522 (99.6%) compounds in this smaller overlapping dataset, but were relatively well correlated with the same (r ( 2 ) = 0.59). TTC-based MoEs were also lower than OED-based MoEs for 590 (83.2%) of the 709 evaluated chemicals, indicating that TTCs may serve as a conservative surrogate in the absence of chemical-specific experimental data. The TTC-based MoE prioritization process was then applied to over 45,000 curated environmental chemical structures as a proof-of-concept for high-throughput prioritization using TTC-based MoEs. This study demonstrates the utility of exploiting existing computational methods at the pre-assessment phase of a tiered risk-based approach to quickly, and conservatively, prioritize thousands of untested chemicals for further study. Frontiers Media S.A. 2022-10-07 /pmc/articles/PMC9586287/ /pubmed/36278238 http://dx.doi.org/10.3389/fphar.2022.980747 Text en Copyright © 2022 Nicolas, Linakis, Minto, Mansouri, Clewell, Yoon, Wambaugh, Patlewicz, McMullen, Andersen and Clewell III. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Pharmacology Nicolas, Chantel I. Linakis, Matthew W. Minto, Melyssa S. Mansouri, Kamel Clewell, Rebecca A. Yoon, Miyoung Wambaugh, John F. Patlewicz, Grace McMullen, Patrick D. Andersen, Melvin E. Clewell III, Harvey J. Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
title | Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
title_full | Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
title_fullStr | Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
title_full_unstemmed | Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
title_short | Estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
title_sort | estimating provisional margins of exposure for data-poor chemicals using high-throughput computational methods |
topic | Pharmacology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586287/ https://www.ncbi.nlm.nih.gov/pubmed/36278238 http://dx.doi.org/10.3389/fphar.2022.980747 |
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