Cargando…
SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14
SWEET sucrose transporters play important roles in the allocation of sucrose in plants. Some SWEETs were shown to also mediate transport of the plant growth regulator gibberellin (GA). The close physiological relationship between sucrose and GA raised the questions of whether there is a functional c...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
National Academy of Sciences
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586311/ https://www.ncbi.nlm.nih.gov/pubmed/36215460 http://dx.doi.org/10.1073/pnas.2207558119 |
_version_ | 1784813664268713984 |
---|---|
author | Isoda, Reika Palmai, Zoltan Yoshinari, Akira Chen, Li-Qing Tama, Florence Frommer, Wolf B. Nakamura, Masayoshi |
author_facet | Isoda, Reika Palmai, Zoltan Yoshinari, Akira Chen, Li-Qing Tama, Florence Frommer, Wolf B. Nakamura, Masayoshi |
author_sort | Isoda, Reika |
collection | PubMed |
description | SWEET sucrose transporters play important roles in the allocation of sucrose in plants. Some SWEETs were shown to also mediate transport of the plant growth regulator gibberellin (GA). The close physiological relationship between sucrose and GA raised the questions of whether there is a functional connection and whether one or both of the substrates are physiologically relevant. To dissect these two activities, molecular dynamics were used to map the binding sites of sucrose and GA in the pore of SWEET13 and predicted binding interactions that might be selective for sucrose or GA. Transport assays confirmed these predictions. In transport assays, the N76Q mutant had 7x higher relative GA(3) activity, and the S142N mutant only transported sucrose. The impaired pollen viability and germination in sweet13;14 double mutants were complemented by the sucrose-selective SWEET13(S142N), but not by the SWEET13(N76Q) mutant, indicating that sucrose is the physiologically relevant substrate and that GA transport capacity is dispensable in the context of male fertility. Therefore, GA supplementation to counter male sterility may act indirectly via stimulating sucrose supply in male sterile mutants. These findings are also relevant in the context of the role of SWEETs in pathogen susceptibility. |
format | Online Article Text |
id | pubmed-9586311 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | National Academy of Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-95863112022-10-22 SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 Isoda, Reika Palmai, Zoltan Yoshinari, Akira Chen, Li-Qing Tama, Florence Frommer, Wolf B. Nakamura, Masayoshi Proc Natl Acad Sci U S A Biological Sciences SWEET sucrose transporters play important roles in the allocation of sucrose in plants. Some SWEETs were shown to also mediate transport of the plant growth regulator gibberellin (GA). The close physiological relationship between sucrose and GA raised the questions of whether there is a functional connection and whether one or both of the substrates are physiologically relevant. To dissect these two activities, molecular dynamics were used to map the binding sites of sucrose and GA in the pore of SWEET13 and predicted binding interactions that might be selective for sucrose or GA. Transport assays confirmed these predictions. In transport assays, the N76Q mutant had 7x higher relative GA(3) activity, and the S142N mutant only transported sucrose. The impaired pollen viability and germination in sweet13;14 double mutants were complemented by the sucrose-selective SWEET13(S142N), but not by the SWEET13(N76Q) mutant, indicating that sucrose is the physiologically relevant substrate and that GA transport capacity is dispensable in the context of male fertility. Therefore, GA supplementation to counter male sterility may act indirectly via stimulating sucrose supply in male sterile mutants. These findings are also relevant in the context of the role of SWEETs in pathogen susceptibility. National Academy of Sciences 2022-10-10 2022-10-18 /pmc/articles/PMC9586311/ /pubmed/36215460 http://dx.doi.org/10.1073/pnas.2207558119 Text en Copyright © 2022 the Author(s). Published by PNAS. https://creativecommons.org/licenses/by-nc-nd/4.0/This open access article is distributed under Creative Commons Attribution-NonCommercial-NoDerivatives License 4.0 (CC BY-NC-ND) (https://creativecommons.org/licenses/by-nc-nd/4.0/) . |
spellingShingle | Biological Sciences Isoda, Reika Palmai, Zoltan Yoshinari, Akira Chen, Li-Qing Tama, Florence Frommer, Wolf B. Nakamura, Masayoshi SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 |
title | SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 |
title_full | SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 |
title_fullStr | SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 |
title_full_unstemmed | SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 |
title_short | SWEET13 transport of sucrose, but not gibberellin, restores male fertility in Arabidopsis sweet13;14 |
title_sort | sweet13 transport of sucrose, but not gibberellin, restores male fertility in arabidopsis sweet13;14 |
topic | Biological Sciences |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586311/ https://www.ncbi.nlm.nih.gov/pubmed/36215460 http://dx.doi.org/10.1073/pnas.2207558119 |
work_keys_str_mv | AT isodareika sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 AT palmaizoltan sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 AT yoshinariakira sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 AT chenliqing sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 AT tamaflorence sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 AT frommerwolfb sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 AT nakamuramasayoshi sweet13transportofsucrosebutnotgibberellinrestoresmalefertilityinarabidopsissweet1314 |