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Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors

Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing saccharification efficiency and rate. Recent studies demonstrate that H(2)O(2) rather than O(2) is the cosubstrate of the LPMO-catalyzed depolymerization of polysaccharides. Some studies have questioned the physiolog...

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Autores principales: Chang, Hucheng, Gacias Amengual, Neus, Botz, Alexander, Schwaiger, Lorenz, Kracher, Daniel, Scheiblbrandner, Stefan, Csarman, Florian, Ludwig, Roland
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586961/
https://www.ncbi.nlm.nih.gov/pubmed/36271009
http://dx.doi.org/10.1038/s41467-022-33963-w
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author Chang, Hucheng
Gacias Amengual, Neus
Botz, Alexander
Schwaiger, Lorenz
Kracher, Daniel
Scheiblbrandner, Stefan
Csarman, Florian
Ludwig, Roland
author_facet Chang, Hucheng
Gacias Amengual, Neus
Botz, Alexander
Schwaiger, Lorenz
Kracher, Daniel
Scheiblbrandner, Stefan
Csarman, Florian
Ludwig, Roland
author_sort Chang, Hucheng
collection PubMed
description Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing saccharification efficiency and rate. Recent studies demonstrate that H(2)O(2) rather than O(2) is the cosubstrate of the LPMO-catalyzed depolymerization of polysaccharides. Some studies have questioned the physiological relevance of the H(2)O(2)-based mechanism for plant cell wall degradation. This study reports the localized and time-resolved determination of LPMO activity on poplar wood cell walls by measuring the H(2)O(2) concentration in their vicinity with a piezo-controlled H(2)O(2) microsensor. The investigated Neurospora crassa LPMO binds to the inner cell wall layer and consumes enzymatically generated H(2)O(2). The results point towards a high catalytic efficiency of LPMO at a low H(2)O(2) concentration that auxiliary oxidoreductases in fungal secretomes can easily generate. Measurements with a glucose microbiosensor additionally demonstrate that LPMO promotes cellobiohydrolase activity on wood cell walls and plays a synergistic role in the fungal extracellular catabolism and in industrial biomass degradation.
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spelling pubmed-95869612022-10-23 Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors Chang, Hucheng Gacias Amengual, Neus Botz, Alexander Schwaiger, Lorenz Kracher, Daniel Scheiblbrandner, Stefan Csarman, Florian Ludwig, Roland Nat Commun Article Lytic polysaccharide monooxygenase (LPMO) supports biomass hydrolysis by increasing saccharification efficiency and rate. Recent studies demonstrate that H(2)O(2) rather than O(2) is the cosubstrate of the LPMO-catalyzed depolymerization of polysaccharides. Some studies have questioned the physiological relevance of the H(2)O(2)-based mechanism for plant cell wall degradation. This study reports the localized and time-resolved determination of LPMO activity on poplar wood cell walls by measuring the H(2)O(2) concentration in their vicinity with a piezo-controlled H(2)O(2) microsensor. The investigated Neurospora crassa LPMO binds to the inner cell wall layer and consumes enzymatically generated H(2)O(2). The results point towards a high catalytic efficiency of LPMO at a low H(2)O(2) concentration that auxiliary oxidoreductases in fungal secretomes can easily generate. Measurements with a glucose microbiosensor additionally demonstrate that LPMO promotes cellobiohydrolase activity on wood cell walls and plays a synergistic role in the fungal extracellular catabolism and in industrial biomass degradation. Nature Publishing Group UK 2022-10-21 /pmc/articles/PMC9586961/ /pubmed/36271009 http://dx.doi.org/10.1038/s41467-022-33963-w Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Chang, Hucheng
Gacias Amengual, Neus
Botz, Alexander
Schwaiger, Lorenz
Kracher, Daniel
Scheiblbrandner, Stefan
Csarman, Florian
Ludwig, Roland
Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
title Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
title_full Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
title_fullStr Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
title_full_unstemmed Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
title_short Investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
title_sort investigating lytic polysaccharide monooxygenase-assisted wood cell wall degradation with microsensors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9586961/
https://www.ncbi.nlm.nih.gov/pubmed/36271009
http://dx.doi.org/10.1038/s41467-022-33963-w
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