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Directed evolution for soluble and active periplasmic expression of bovine enterokinase in Escherichia coli

Bovine enterokinase light chain (EK(L)) is an industrially useful protease for accurate removal of affinity-purification tags from high-value biopharmaceuticals. However, recombinant expression in Escherichia coli produces insoluble inclusion bodies, requiring solubilisation, refolding, and autocata...

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Detalles Bibliográficos
Autores principales:	Lee, Weiluo, Pradhan, Subhas, Zhang, Cheng, Venanzi, Niccolo A. E., Li, Weina, Goldrick, Stephen, Dalby, Paul A.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group UK 2022
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587228/ https://www.ncbi.nlm.nih.gov/pubmed/36271247 http://dx.doi.org/10.1038/s41598-022-22574-6

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