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Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps

BACKGROUND: The identification of type-2 inflammation in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) acquires a crucial role in the endotypization needed for selecting patients for biological drugs targeting type-2 inflammation: to date, the parameters used include systemic and h...

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Autores principales: Paoletti, Giovanni, Malvezzi, Luca, Riccio, Anna Maria, Descalzi, Desideria, Pirola, Francesca, Russo, Elena, De Ferrari, Laura, Racca, Francesca, Ferri, Sebastian, Messina, Maria Rita, Puggioni, Francesca, Nappi, Emanuele, Bagnasco, Diego, Canevari, Frank Rikki, Grizzi, Fabio, Mercante, Giuseppe, Spriano, Giuseppe, Canonica, Giorgio Walter, Heffler, Enrico
Formato: Online Artículo Texto
Lenguaje:English
Publicado: World Allergy Organization 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587370/
https://www.ncbi.nlm.nih.gov/pubmed/36321070
http://dx.doi.org/10.1016/j.waojou.2022.100700
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author Paoletti, Giovanni
Malvezzi, Luca
Riccio, Anna Maria
Descalzi, Desideria
Pirola, Francesca
Russo, Elena
De Ferrari, Laura
Racca, Francesca
Ferri, Sebastian
Messina, Maria Rita
Puggioni, Francesca
Nappi, Emanuele
Bagnasco, Diego
Canevari, Frank Rikki
Grizzi, Fabio
Mercante, Giuseppe
Spriano, Giuseppe
Canonica, Giorgio Walter
Heffler, Enrico
author_facet Paoletti, Giovanni
Malvezzi, Luca
Riccio, Anna Maria
Descalzi, Desideria
Pirola, Francesca
Russo, Elena
De Ferrari, Laura
Racca, Francesca
Ferri, Sebastian
Messina, Maria Rita
Puggioni, Francesca
Nappi, Emanuele
Bagnasco, Diego
Canevari, Frank Rikki
Grizzi, Fabio
Mercante, Giuseppe
Spriano, Giuseppe
Canonica, Giorgio Walter
Heffler, Enrico
author_sort Paoletti, Giovanni
collection PubMed
description BACKGROUND: The identification of type-2 inflammation in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) acquires a crucial role in the endotypization needed for selecting patients for biological drugs targeting type-2 inflammation: to date, the parameters used include systemic and histological biomarkers. The aim of this study was to investigate whether nasal cytology could identify type-2 inflammation in patients with CRSwNP. METHODOLOGY: Thirty-three consecutive patients with CRSwNP underwent nasal cytology sampling at the level of the lower nasal turbinate, and of the polypoid tissue, and surgical polyp tissue sample was collected. The cellularity of the 3 collected samples were compared. RESULTS: Mean nasal polyp tissue, nasal polyps cytology and inferior turbinate cytology eosinophils counts were 43.7 ± 39.6 cells/HPF, 32.8 ± 44.7 cells/HPF and 27.6 ± 58.0 cells/HPF respectively with inferior turbinate cytology eosinophils significantly lower than nasal polyp tissue count (p = 0.007). Both mean nasal polyps cytology eosinophils and mean inferior turbinate cytology eosinophils were significantly higher in patients with type-2 CRSwNP (52.5 ± 67.0 cells/HPF vs 12.2 ± 17.3 cells/HPF, p = 0.012, and 32.0 ± 62.1 cells/HPF vs 2.9 ± 2.9 cells/HPF, p = 0.020 respectively). CONCLUSIONS: Nasal cytology is suitable tool for assessing local biomarkers of type-2 inflammation in CRSwNP.
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spelling pubmed-95873702022-10-31 Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps Paoletti, Giovanni Malvezzi, Luca Riccio, Anna Maria Descalzi, Desideria Pirola, Francesca Russo, Elena De Ferrari, Laura Racca, Francesca Ferri, Sebastian Messina, Maria Rita Puggioni, Francesca Nappi, Emanuele Bagnasco, Diego Canevari, Frank Rikki Grizzi, Fabio Mercante, Giuseppe Spriano, Giuseppe Canonica, Giorgio Walter Heffler, Enrico World Allergy Organ J Full-Length Article BACKGROUND: The identification of type-2 inflammation in patients with chronic rhinosinusitis with nasal polyps (CRSwNP) acquires a crucial role in the endotypization needed for selecting patients for biological drugs targeting type-2 inflammation: to date, the parameters used include systemic and histological biomarkers. The aim of this study was to investigate whether nasal cytology could identify type-2 inflammation in patients with CRSwNP. METHODOLOGY: Thirty-three consecutive patients with CRSwNP underwent nasal cytology sampling at the level of the lower nasal turbinate, and of the polypoid tissue, and surgical polyp tissue sample was collected. The cellularity of the 3 collected samples were compared. RESULTS: Mean nasal polyp tissue, nasal polyps cytology and inferior turbinate cytology eosinophils counts were 43.7 ± 39.6 cells/HPF, 32.8 ± 44.7 cells/HPF and 27.6 ± 58.0 cells/HPF respectively with inferior turbinate cytology eosinophils significantly lower than nasal polyp tissue count (p = 0.007). Both mean nasal polyps cytology eosinophils and mean inferior turbinate cytology eosinophils were significantly higher in patients with type-2 CRSwNP (52.5 ± 67.0 cells/HPF vs 12.2 ± 17.3 cells/HPF, p = 0.012, and 32.0 ± 62.1 cells/HPF vs 2.9 ± 2.9 cells/HPF, p = 0.020 respectively). CONCLUSIONS: Nasal cytology is suitable tool for assessing local biomarkers of type-2 inflammation in CRSwNP. World Allergy Organization 2022-10-18 /pmc/articles/PMC9587370/ /pubmed/36321070 http://dx.doi.org/10.1016/j.waojou.2022.100700 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full-Length Article
Paoletti, Giovanni
Malvezzi, Luca
Riccio, Anna Maria
Descalzi, Desideria
Pirola, Francesca
Russo, Elena
De Ferrari, Laura
Racca, Francesca
Ferri, Sebastian
Messina, Maria Rita
Puggioni, Francesca
Nappi, Emanuele
Bagnasco, Diego
Canevari, Frank Rikki
Grizzi, Fabio
Mercante, Giuseppe
Spriano, Giuseppe
Canonica, Giorgio Walter
Heffler, Enrico
Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
title Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
title_full Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
title_fullStr Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
title_full_unstemmed Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
title_short Nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
title_sort nasal cytology as a reliable non-invasive procedure to phenotype patients with type 2 chronic rhinosinusitis with nasal polyps
topic Full-Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587370/
https://www.ncbi.nlm.nih.gov/pubmed/36321070
http://dx.doi.org/10.1016/j.waojou.2022.100700
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