Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads

Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employe...

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Detalles Bibliográficos
Autores principales: Ivanusic, Daniel, Madela, Kazimierz, Bannert, Norbert, Denner, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587459/
https://www.ncbi.nlm.nih.gov/pubmed/36281316
http://dx.doi.org/10.17912/micropub.biology.000648
Descripción
Sumario:Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1).

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