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Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employe...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Caltech Library
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587459/ https://www.ncbi.nlm.nih.gov/pubmed/36281316 http://dx.doi.org/10.17912/micropub.biology.000648 |
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author | Ivanusic, Daniel Madela, Kazimierz Bannert, Norbert Denner, Joachim |
author_facet | Ivanusic, Daniel Madela, Kazimierz Bannert, Norbert Denner, Joachim |
author_sort | Ivanusic, Daniel |
collection | PubMed |
description | Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1). |
format | Online Article Text |
id | pubmed-9587459 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Caltech Library |
record_format | MEDLINE/PubMed |
spelling | pubmed-95874592022-10-23 Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads Ivanusic, Daniel Madela, Kazimierz Bannert, Norbert Denner, Joachim MicroPubl Biol New Methods Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1). Caltech Library 2022-10-07 /pmc/articles/PMC9587459/ /pubmed/36281316 http://dx.doi.org/10.17912/micropub.biology.000648 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | New Methods Ivanusic, Daniel Madela, Kazimierz Bannert, Norbert Denner, Joachim Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads |
title | Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads |
title_full | Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads |
title_fullStr | Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads |
title_full_unstemmed | Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads |
title_short | Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads |
title_sort | time-lapse imaging of cd63 dynamics at the hiv-1 virological synapse by using agar pads |
topic | New Methods |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587459/ https://www.ncbi.nlm.nih.gov/pubmed/36281316 http://dx.doi.org/10.17912/micropub.biology.000648 |
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