Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads

Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employe...

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Detalles Bibliográficos
Autores principales: Ivanusic, Daniel, Madela, Kazimierz, Bannert, Norbert, Denner, Joachim
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Caltech Library 2022
Materias:
New Methods
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587459/
https://www.ncbi.nlm.nih.gov/pubmed/36281316
http://dx.doi.org/10.17912/micropub.biology.000648
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author Ivanusic, Daniel
Madela, Kazimierz
Bannert, Norbert
Denner, Joachim
author_facet Ivanusic, Daniel
Madela, Kazimierz
Bannert, Norbert
Denner, Joachim
author_sort Ivanusic, Daniel
collection PubMed
description Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1).
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spelling pubmed-95874592022-10-23 Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads Ivanusic, Daniel Madela, Kazimierz Bannert, Norbert Denner, Joachim MicroPubl Biol New Methods Time-lapse imaging provides an uninterrupted observation method that can lead to understanding protein dynamics. We previously developed a technique based on thin agar pads to keep the cells in focus during confocal laser scanning microscope imaging. Using this method, time-lapse imaging was employed to monitor CD63 fused to mCherry at the virological synapse (VS) during viral cluster transfer to acceptor cells of the human immunodeficiency virus 1 (HIV-1). Caltech Library 2022-10-07 /pmc/articles/PMC9587459/ /pubmed/36281316 http://dx.doi.org/10.17912/micropub.biology.000648 Text en Copyright: © 2022 by the authors https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle New Methods
Ivanusic, Daniel
Madela, Kazimierz
Bannert, Norbert
Denner, Joachim
Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
title Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
title_full Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
title_fullStr Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
title_full_unstemmed Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
title_short Time-lapse imaging of CD63 dynamics at the HIV-1 virological synapse by using agar pads
title_sort time-lapse imaging of cd63 dynamics at the hiv-1 virological synapse by using agar pads
topic New Methods
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587459/
https://www.ncbi.nlm.nih.gov/pubmed/36281316
http://dx.doi.org/10.17912/micropub.biology.000648
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AT bannertnorbert timelapseimagingofcd63dynamicsatthehiv1virologicalsynapsebyusingagarpads
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