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Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer

OBJECTIVE: Breast cancer (BC) currently has no effective treatment especially for the highly aggressive and metastatic triple negative breast cancer (TNBC). Here, we investigated the antitumoral and antimigratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therap...

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Autores principales: de Souza, Maria VF, Shinobu-Mesquita, Cristiane S, Meirelles, Lyvia EF, Mari, Natália L, César, Gabriel B, Gonçalves, Renato S, Caetano, Wilker, Damke, Edilson, Silva, Vânia RS, Damke, Gabrielle MZF, Consolaro, Marcia EL
Formato: Online Artículo Texto
Lenguaje:English
Publicado: West Asia Organization for Cancer Prevention 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587883/
https://www.ncbi.nlm.nih.gov/pubmed/35633560
http://dx.doi.org/10.31557/APJCP.2022.23.5.1741
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author de Souza, Maria VF
Shinobu-Mesquita, Cristiane S
Meirelles, Lyvia EF
Mari, Natália L
César, Gabriel B
Gonçalves, Renato S
Caetano, Wilker
Damke, Edilson
Silva, Vânia RS
Damke, Gabrielle MZF
Consolaro, Marcia EL
author_facet de Souza, Maria VF
Shinobu-Mesquita, Cristiane S
Meirelles, Lyvia EF
Mari, Natália L
César, Gabriel B
Gonçalves, Renato S
Caetano, Wilker
Damke, Edilson
Silva, Vânia RS
Damke, Gabrielle MZF
Consolaro, Marcia EL
author_sort de Souza, Maria VF
collection PubMed
description OBJECTIVE: Breast cancer (BC) currently has no effective treatment especially for the highly aggressive and metastatic triple negative breast cancer (TNBC). Here, we investigated the antitumoral and antimigratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against TNBC cell line MDA-MB-231 compared to a nontumorigenic human breast ductal cell line (MCF-10A). METHODS: The phototoxicity/cytotoxicity was assessed by MTT assay, long-term cytotoxicity by clonogenic assay, cell uptake, subcellular distribution, and cellular oxidative stress by fluorescence microscopy, cell death with annexin V-FITC/propidium iodide, PDT mechanism using sodium azide and D-mannitol, and cell migration by wound-healing assay. RESULTS: The treatment promoted phototoxic effect on tumor cell line in a dose-dependent and selective manner. Internalization of F127/HYP was efficient and accumulation occurred in the endoplasmic reticulum and mitochondria, resulting in cellular oxidative stress mainly by the type II mechanism, induced by necrosis. Furthermore, F127/HYP decreased colony formation and reduced the cell migration ability in MDA-MB-231 cells. CONCLUSION: Our results suggest a potentially useful role of F127/HYP micelles as a platform for HYP delivery to more specifically and effectively treat TNBC.
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spelling pubmed-95878832022-10-28 Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer de Souza, Maria VF Shinobu-Mesquita, Cristiane S Meirelles, Lyvia EF Mari, Natália L César, Gabriel B Gonçalves, Renato S Caetano, Wilker Damke, Edilson Silva, Vânia RS Damke, Gabrielle MZF Consolaro, Marcia EL Asian Pac J Cancer Prev Research Article OBJECTIVE: Breast cancer (BC) currently has no effective treatment especially for the highly aggressive and metastatic triple negative breast cancer (TNBC). Here, we investigated the antitumoral and antimigratory effects of hypericin (HYP) encapsulated on Pluronic F127 (F127/HYP) photodynamic therapy (PDT) against TNBC cell line MDA-MB-231 compared to a nontumorigenic human breast ductal cell line (MCF-10A). METHODS: The phototoxicity/cytotoxicity was assessed by MTT assay, long-term cytotoxicity by clonogenic assay, cell uptake, subcellular distribution, and cellular oxidative stress by fluorescence microscopy, cell death with annexin V-FITC/propidium iodide, PDT mechanism using sodium azide and D-mannitol, and cell migration by wound-healing assay. RESULTS: The treatment promoted phototoxic effect on tumor cell line in a dose-dependent and selective manner. Internalization of F127/HYP was efficient and accumulation occurred in the endoplasmic reticulum and mitochondria, resulting in cellular oxidative stress mainly by the type II mechanism, induced by necrosis. Furthermore, F127/HYP decreased colony formation and reduced the cell migration ability in MDA-MB-231 cells. CONCLUSION: Our results suggest a potentially useful role of F127/HYP micelles as a platform for HYP delivery to more specifically and effectively treat TNBC. West Asia Organization for Cancer Prevention 2022-05 /pmc/articles/PMC9587883/ /pubmed/35633560 http://dx.doi.org/10.31557/APJCP.2022.23.5.1741 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-Non Commercial 4.0 International License. https://creativecommons.org/licenses/by-nc/4.0/
spellingShingle Research Article
de Souza, Maria VF
Shinobu-Mesquita, Cristiane S
Meirelles, Lyvia EF
Mari, Natália L
César, Gabriel B
Gonçalves, Renato S
Caetano, Wilker
Damke, Edilson
Silva, Vânia RS
Damke, Gabrielle MZF
Consolaro, Marcia EL
Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer
title Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer
title_full Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer
title_fullStr Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer
title_full_unstemmed Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer
title_short Effects of Hypericin Encapsulated on Pluronic F127 Photodynamic Therapy Against Triple Negative Breast Cancer
title_sort effects of hypericin encapsulated on pluronic f127 photodynamic therapy against triple negative breast cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9587883/
https://www.ncbi.nlm.nih.gov/pubmed/35633560
http://dx.doi.org/10.31557/APJCP.2022.23.5.1741
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