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EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala

Fear memory may undergo a process after memory reactivation called reconsolidation. To examine the roles of ephrinA4 in fear memory reconsolidation an inhibitory ephrinA4 mimetic peptide (pep-ephrinA4), that targets the EphA binding site and inhibits EphA activation, was used. Pep-ephrinA4 was micro...

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Autores principales: Mana, Ron, Ilovich, Or, Dines, Monica, Lamprecht, Raphael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588004/
https://www.ncbi.nlm.nih.gov/pubmed/36273074
http://dx.doi.org/10.1038/s41598-022-21519-3
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author Mana, Ron
Ilovich, Or
Dines, Monica
Lamprecht, Raphael
author_facet Mana, Ron
Ilovich, Or
Dines, Monica
Lamprecht, Raphael
author_sort Mana, Ron
collection PubMed
description Fear memory may undergo a process after memory reactivation called reconsolidation. To examine the roles of ephrinA4 in fear memory reconsolidation an inhibitory ephrinA4 mimetic peptide (pep-ephrinA4), that targets the EphA binding site and inhibits EphA activation, was used. Pep-ephrinA4 was microinjected into the lateral amygdala (LA) of fear-conditioned rats 24 h after training and 30 min before tone CS memory retrieval. Memory retrieval was unaffected by pep-ephrinA4. However, the animals were impaired in fear memory tested 1 h or 24 h afterward when compared to controls. Fear-conditioned animals injected with pep-ephrinA4 into LA immediately after long-term memory retrieval were unaffected when tested 24 h afterward. Microinjection into LA of a peptide originated from an ephrinA4 site that does not interact with EphA did not affect fear memory reconsolidation. Rats that were administrated with pep-ephrinA4 systemically 24 h after fear conditioning and 30 min before CS memory retrieval were impaired in long-term fear conditioning memory tested 24 h afterward when compared to the control peptide. These results show that ephrinA4 binding sites are needed for long-term fear memory reconsolidation in LA and may serve as a target for the treatment of fear-related disorders by blocking reconsolidation.
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spelling pubmed-95880042022-10-24 EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala Mana, Ron Ilovich, Or Dines, Monica Lamprecht, Raphael Sci Rep Article Fear memory may undergo a process after memory reactivation called reconsolidation. To examine the roles of ephrinA4 in fear memory reconsolidation an inhibitory ephrinA4 mimetic peptide (pep-ephrinA4), that targets the EphA binding site and inhibits EphA activation, was used. Pep-ephrinA4 was microinjected into the lateral amygdala (LA) of fear-conditioned rats 24 h after training and 30 min before tone CS memory retrieval. Memory retrieval was unaffected by pep-ephrinA4. However, the animals were impaired in fear memory tested 1 h or 24 h afterward when compared to controls. Fear-conditioned animals injected with pep-ephrinA4 into LA immediately after long-term memory retrieval were unaffected when tested 24 h afterward. Microinjection into LA of a peptide originated from an ephrinA4 site that does not interact with EphA did not affect fear memory reconsolidation. Rats that were administrated with pep-ephrinA4 systemically 24 h after fear conditioning and 30 min before CS memory retrieval were impaired in long-term fear conditioning memory tested 24 h afterward when compared to the control peptide. These results show that ephrinA4 binding sites are needed for long-term fear memory reconsolidation in LA and may serve as a target for the treatment of fear-related disorders by blocking reconsolidation. Nature Publishing Group UK 2022-10-22 /pmc/articles/PMC9588004/ /pubmed/36273074 http://dx.doi.org/10.1038/s41598-022-21519-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Mana, Ron
Ilovich, Or
Dines, Monica
Lamprecht, Raphael
EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
title EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
title_full EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
title_fullStr EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
title_full_unstemmed EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
title_short EphrinA4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
title_sort ephrina4 mimetic peptide impairs fear conditioning memory reconsolidation in lateral amygdala
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588004/
https://www.ncbi.nlm.nih.gov/pubmed/36273074
http://dx.doi.org/10.1038/s41598-022-21519-3
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