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An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds

OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin regeneration in small wounds. MATERIALS AND METHODS: In thi...

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Autores principales: Rahmati, Shima, Jalili, Ali, Banitalebi Dehkordi, Mehdi, Przedborski, Michelle
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588162/
https://www.ncbi.nlm.nih.gov/pubmed/36274203
http://dx.doi.org/10.22074/cellj.2022.8005
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author Rahmati, Shima
Jalili, Ali
Banitalebi Dehkordi, Mehdi
Przedborski, Michelle
author_facet Rahmati, Shima
Jalili, Ali
Banitalebi Dehkordi, Mehdi
Przedborski, Michelle
author_sort Rahmati, Shima
collection PubMed
description OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin regeneration in small wounds. MATERIALS AND METHODS: In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimized foreskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility and recellularization of both scaffolds by hucMSC were subsequently determined. RESULTS: We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength. CONCLUSION: We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and the mechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small wounds.
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spelling pubmed-95881622022-10-28 An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds Rahmati, Shima Jalili, Ali Banitalebi Dehkordi, Mehdi Przedborski, Michelle Cell J Original Article OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin regeneration in small wounds. MATERIALS AND METHODS: In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimized foreskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility and recellularization of both scaffolds by hucMSC were subsequently determined. RESULTS: We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength. CONCLUSION: We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and the mechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small wounds. Royan Institute 2022-09 2022-09-12 /pmc/articles/PMC9588162/ /pubmed/36274203 http://dx.doi.org/10.22074/cellj.2022.8005 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Rahmati, Shima
Jalili, Ali
Banitalebi Dehkordi, Mehdi
Przedborski, Michelle
An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
title An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
title_full An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
title_fullStr An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
title_full_unstemmed An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
title_short An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
title_sort effective method for decellularization of human foreskin: implications for skin regeneration in small wounds
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588162/
https://www.ncbi.nlm.nih.gov/pubmed/36274203
http://dx.doi.org/10.22074/cellj.2022.8005
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