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An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds
OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin regeneration in small wounds. MATERIALS AND METHODS: In thi...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Royan Institute
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588162/ https://www.ncbi.nlm.nih.gov/pubmed/36274203 http://dx.doi.org/10.22074/cellj.2022.8005 |
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author | Rahmati, Shima Jalili, Ali Banitalebi Dehkordi, Mehdi Przedborski, Michelle |
author_facet | Rahmati, Shima Jalili, Ali Banitalebi Dehkordi, Mehdi Przedborski, Michelle |
author_sort | Rahmati, Shima |
collection | PubMed |
description | OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin regeneration in small wounds. MATERIALS AND METHODS: In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimized foreskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility and recellularization of both scaffolds by hucMSC were subsequently determined. RESULTS: We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength. CONCLUSION: We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and the mechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small wounds. |
format | Online Article Text |
id | pubmed-9588162 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Royan Institute |
record_format | MEDLINE/PubMed |
spelling | pubmed-95881622022-10-28 An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds Rahmati, Shima Jalili, Ali Banitalebi Dehkordi, Mehdi Przedborski, Michelle Cell J Original Article OBJECTIVE: Acellular matrices of different allogeneic or xenogeneic origins are widely used as structural scaffolds in regenerative medicine. The main goal of this research was to optimize a method for decellularization of foreskin for skin regeneration in small wounds. MATERIALS AND METHODS: In this experimental study, the dermal layers of foreskin were divided into two sections and subjected to two different decellularization methods: the sodium dodecyl sulfate method (SDS-M), and our optimized foreskin decellularization method (OFD-M). A combination of non-ionic detergents and SDS were used to decellularize the foreskin in OFD-M. The histological, morphological, and biomechanical properties of both methods were compared. In addition, human umbilical cord mesenchymal stem cells (hucMSCs) were isolated, and the biocompatibility and recellularization of both scaffolds by hucMSC were subsequently determined. RESULTS: We observed that OFD-M is an appropriate approach for successful removal of cellular components from the foreskin tissue, without physical disturbance to the acellular matrix. In comparison to SDS-M, this new bioscaffold possesses a fine network containing a high amount of collagen fibers and glycosaminoglycans (GAG) (P≤0.03), is biocompatible and harmless for hucMSC (viability 91.7%), and exhibits a relatively high tensile strength. CONCLUSION: We found that the extracellular matrix (ECM) structural integrity, the main ECM components, and the mechanical properties of the foreskin are well maintained after applying the OFD-M decellularization technique, indicating that the resulting scaffold would be a suitable platform for culturing MSC for skin grafting in small wounds. Royan Institute 2022-09 2022-09-12 /pmc/articles/PMC9588162/ /pubmed/36274203 http://dx.doi.org/10.22074/cellj.2022.8005 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Rahmati, Shima Jalili, Ali Banitalebi Dehkordi, Mehdi Przedborski, Michelle An Effective Method for Decellularization of Human Foreskin: Implications for Skin Regeneration in Small Wounds |
title | An Effective Method for Decellularization of Human Foreskin:
Implications for Skin Regeneration in Small Wounds |
title_full | An Effective Method for Decellularization of Human Foreskin:
Implications for Skin Regeneration in Small Wounds |
title_fullStr | An Effective Method for Decellularization of Human Foreskin:
Implications for Skin Regeneration in Small Wounds |
title_full_unstemmed | An Effective Method for Decellularization of Human Foreskin:
Implications for Skin Regeneration in Small Wounds |
title_short | An Effective Method for Decellularization of Human Foreskin:
Implications for Skin Regeneration in Small Wounds |
title_sort | effective method for decellularization of human foreskin:
implications for skin regeneration in small wounds |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588162/ https://www.ncbi.nlm.nih.gov/pubmed/36274203 http://dx.doi.org/10.22074/cellj.2022.8005 |
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