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The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer

METHODS: The differential expressed genes (DEGs) were screened from the gene expression profile GSE30994 related to PRAD and then analyzed by protein-protein interaction (PPI) to screen the hub gene. Subsequently, the relation between hub gene and pan cancers, PRAD prognosis, and immunotherapy was a...

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Autores principales: Zhou, Bing, Song, Hong, Xu, Wuqin, Zhang, Yanbin, Liu, Yinhua, Qi, Wei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588343/
https://www.ncbi.nlm.nih.gov/pubmed/36284990
http://dx.doi.org/10.1155/2022/8518378
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author Zhou, Bing
Song, Hong
Xu, Wuqin
Zhang, Yanbin
Liu, Yinhua
Qi, Wei
author_facet Zhou, Bing
Song, Hong
Xu, Wuqin
Zhang, Yanbin
Liu, Yinhua
Qi, Wei
author_sort Zhou, Bing
collection PubMed
description METHODS: The differential expressed genes (DEGs) were screened from the gene expression profile GSE30994 related to PRAD and then analyzed by protein-protein interaction (PPI) to screen the hub gene. Subsequently, the relation between hub gene and pan cancers, PRAD prognosis, and immunotherapy was analyzed. Besides, the effects of hub gene on the growth and metastasis of PRAD cell lines and inflammatory factors (IFs) were detected by functional experiments. RESULTS: 276 upregulated and 1,861 downregulated DEGs were analyzed from GSE30994 gene expression profiles. Through enrichment analysis, it was found that upregulated DEGs were significantly enriched in nitric oxide-mediated signal transduction, insulin signaling pathway, etc. Through PPI networks, ARRB2 was determined as the hub gene that was highly expressed in pan cancers, including PRAD, and contributed to poor prognosis of PRAD patients. Immunoassay showed that ARRB2 was associated with B cells, NK cells, endothelial cells, etc. and also connected with tumor-infiltrating lymphocytes (TILs). Next, the signature model analysis revealed that ARRB2 had a clinical value in predicting PRAD prognosis. In functional experiments, ARRB2 was highly expressed in PRAD cell lines, promoted PRAD cell growth and metastasis, and positively associated with IFs. CONCLUSION: ARRB2 has a good prognostic ability in PRAD, and it could be a potential target of PRAD immunotherapy, which offers new directions for PRAD research.
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spelling pubmed-95883432022-10-24 The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer Zhou, Bing Song, Hong Xu, Wuqin Zhang, Yanbin Liu, Yinhua Qi, Wei Dis Markers Research Article METHODS: The differential expressed genes (DEGs) were screened from the gene expression profile GSE30994 related to PRAD and then analyzed by protein-protein interaction (PPI) to screen the hub gene. Subsequently, the relation between hub gene and pan cancers, PRAD prognosis, and immunotherapy was analyzed. Besides, the effects of hub gene on the growth and metastasis of PRAD cell lines and inflammatory factors (IFs) were detected by functional experiments. RESULTS: 276 upregulated and 1,861 downregulated DEGs were analyzed from GSE30994 gene expression profiles. Through enrichment analysis, it was found that upregulated DEGs were significantly enriched in nitric oxide-mediated signal transduction, insulin signaling pathway, etc. Through PPI networks, ARRB2 was determined as the hub gene that was highly expressed in pan cancers, including PRAD, and contributed to poor prognosis of PRAD patients. Immunoassay showed that ARRB2 was associated with B cells, NK cells, endothelial cells, etc. and also connected with tumor-infiltrating lymphocytes (TILs). Next, the signature model analysis revealed that ARRB2 had a clinical value in predicting PRAD prognosis. In functional experiments, ARRB2 was highly expressed in PRAD cell lines, promoted PRAD cell growth and metastasis, and positively associated with IFs. CONCLUSION: ARRB2 has a good prognostic ability in PRAD, and it could be a potential target of PRAD immunotherapy, which offers new directions for PRAD research. Hindawi 2022-10-15 /pmc/articles/PMC9588343/ /pubmed/36284990 http://dx.doi.org/10.1155/2022/8518378 Text en Copyright © 2022 Bing Zhou et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Zhou, Bing
Song, Hong
Xu, Wuqin
Zhang, Yanbin
Liu, Yinhua
Qi, Wei
The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer
title The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer
title_full The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer
title_fullStr The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer
title_full_unstemmed The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer
title_short The Comprehensive Analysis of Hub Gene ARRB2 in Prostate Cancer
title_sort comprehensive analysis of hub gene arrb2 in prostate cancer
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9588343/
https://www.ncbi.nlm.nih.gov/pubmed/36284990
http://dx.doi.org/10.1155/2022/8518378
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