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Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk
AIM: The current study aimed to remove aflatoxin from reconstituted milk by adding three probiotics, namely Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus. BACKGROUND: Aflatoxins are poisonous substances produced by certain kinds of fungi that are found naturally all ove...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Shaheed Beheshti University of Medical Sciences
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589129/ https://www.ncbi.nlm.nih.gov/pubmed/36311958 http://dx.doi.org/10.22037/ghfbb.v15i3.2402 |
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author | Rezasoltani, Sama Amir Ebrahimi, Niloufar Khadivi Boroujeni, Reza Asadzadeh Aghdaei, Hamid Norouzinia, Mohsen |
author_facet | Rezasoltani, Sama Amir Ebrahimi, Niloufar Khadivi Boroujeni, Reza Asadzadeh Aghdaei, Hamid Norouzinia, Mohsen |
author_sort | Rezasoltani, Sama |
collection | PubMed |
description | AIM: The current study aimed to remove aflatoxin from reconstituted milk by adding three probiotics, namely Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus. BACKGROUND: Aflatoxins are poisonous substances produced by certain kinds of fungi that are found naturally all over the world. They can contaminate food crops and pose a serious health threat to humans and livestock. Microbial detoxification is one method of eliminating aflatoxins, including aflatoxin M1. METHODS: For this purpose, about 109 and 107 cfu/ml of S. boulardii, L. casei, and L. acidophilus were inoculated into skim milk without aflatoxin M1. The samples were then spiked by aflatoxin M1 in concentrations of 0.5 and 0.75 ng/ml. The concentration of the aflatoxin residing in supernatant of milk samples after different storage times (30 and 90 minutes) and temperatures of 4 ℃ and 37 °C was measured by ELISA method, and the results were confirmed by HPLC. RESULTS: The results showed that the highest amount of aflatoxin M1 removal was related to S. boulardii (96.88 ± 3.79c) with a microbial density concentration of 109 cfu/ml and toxin concentration of 0.75 ng/ml at 37 °C for 90 minutes and then to L. acidophilus (71.46 ± 3.79b) with a microbial density concentration of 107 cfu/ml and toxin concentration 0.75 ng/ml at 4 °C for 90 minutes. Furthermore, the maximum level of AFM1 binding to 107 cfu/ml of L. casei with average binding percentages of 64.31 ± 3/79c was 0.75 ng/ml at 37 °C for 90 minutes. CONCLUSION: The results revealed the possibility of using S. boulardii in combination with the selected probiotics of L. casei and L. acidophilus in the detoxification of AFM1-contaminated milk. |
format | Online Article Text |
id | pubmed-9589129 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Shaheed Beheshti University of Medical Sciences |
record_format | MEDLINE/PubMed |
spelling | pubmed-95891292022-10-28 Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk Rezasoltani, Sama Amir Ebrahimi, Niloufar Khadivi Boroujeni, Reza Asadzadeh Aghdaei, Hamid Norouzinia, Mohsen Gastroenterol Hepatol Bed Bench Original Article AIM: The current study aimed to remove aflatoxin from reconstituted milk by adding three probiotics, namely Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus. BACKGROUND: Aflatoxins are poisonous substances produced by certain kinds of fungi that are found naturally all over the world. They can contaminate food crops and pose a serious health threat to humans and livestock. Microbial detoxification is one method of eliminating aflatoxins, including aflatoxin M1. METHODS: For this purpose, about 109 and 107 cfu/ml of S. boulardii, L. casei, and L. acidophilus were inoculated into skim milk without aflatoxin M1. The samples were then spiked by aflatoxin M1 in concentrations of 0.5 and 0.75 ng/ml. The concentration of the aflatoxin residing in supernatant of milk samples after different storage times (30 and 90 minutes) and temperatures of 4 ℃ and 37 °C was measured by ELISA method, and the results were confirmed by HPLC. RESULTS: The results showed that the highest amount of aflatoxin M1 removal was related to S. boulardii (96.88 ± 3.79c) with a microbial density concentration of 109 cfu/ml and toxin concentration of 0.75 ng/ml at 37 °C for 90 minutes and then to L. acidophilus (71.46 ± 3.79b) with a microbial density concentration of 107 cfu/ml and toxin concentration 0.75 ng/ml at 4 °C for 90 minutes. Furthermore, the maximum level of AFM1 binding to 107 cfu/ml of L. casei with average binding percentages of 64.31 ± 3/79c was 0.75 ng/ml at 37 °C for 90 minutes. CONCLUSION: The results revealed the possibility of using S. boulardii in combination with the selected probiotics of L. casei and L. acidophilus in the detoxification of AFM1-contaminated milk. Shaheed Beheshti University of Medical Sciences 2022 /pmc/articles/PMC9589129/ /pubmed/36311958 http://dx.doi.org/10.22037/ghfbb.v15i3.2402 Text en ©2022 RIGLD, Research Institute for Gastroenterology and Liver Diseases https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article, distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) which permits others to copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Original Article Rezasoltani, Sama Amir Ebrahimi, Niloufar Khadivi Boroujeni, Reza Asadzadeh Aghdaei, Hamid Norouzinia, Mohsen Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk |
title | Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk |
title_full | Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk |
title_fullStr | Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk |
title_full_unstemmed | Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk |
title_short | Detoxification of aflatoxin M1 by probiotics Saccharomyces boulardii, Lactobacillus casei, and Lactobacillus acidophilus in reconstituted milk |
title_sort | detoxification of aflatoxin m1 by probiotics saccharomyces boulardii, lactobacillus casei, and lactobacillus acidophilus in reconstituted milk |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9589129/ https://www.ncbi.nlm.nih.gov/pubmed/36311958 http://dx.doi.org/10.22037/ghfbb.v15i3.2402 |
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